Descripción: Considering the high concentration of rock avalanches in the northern part of the Neuquén province of Argentina (36°-38°S and 70°-71°W) and their association with lacustrine basins forming natural dams, their catastrophic collapse seems not to be such a common process as could be expected for these cases. The present work depicts the area immediately affected by the outburst flood corresponding to the Navarrete dam catastrophic collapse (175 × 106 m3), through a sedimentological analysis, generated since the arroyo Colorado was blocked due to a rock avalanche deposit during not well constrained postglacial times. The outburst flood dispersion is initially inferred based on morphological criteria and then determined from a sedimentological analysis of its matrix. Furthermore, the matrix is compared to that of the fluvial terraces, which have been sampled along the arroyo Colorado. This comparison shows that even though differences among them are not so significant through most of the study area, matrix frequency starts to be distinctive from the most distant point of sampling.

Descripción: Dimethyl sulfoxide (DMSO) has been known to enhance cell membrane permeability of drugs or DNA. Molecular dynamics (MD) simulations with single-component lipid bilayers predicted the existence of three regimes of action of DMSO: membrane loosening, pore formation and bilayer collapse. We show here that these modes of action are also reproduced in the presence of cholesterol in the bilayer, and we provide a description at the atomic detail of the DMSO-mediated process of pore formation in cholesterol-containing lipid membranes. We also successfully explore the applicability of DMSO to promote plasma membrane permeability to water, calcium ions (Ca2+) and Yo-Pro-1 iodide (Yo-Pro-1) in living cell membranes. The experimental results on cells in culture can be easily explained according to the three expected regimes: in the presence of low doses of DMSO, the membrane of the cells exhibits undulations but no permeability increase can be detected, while at intermediate DMSO concentrations cells are permeabilized to water and calcium but not to larger molecules as Yo-Pro-1. These two behaviors can be associated to the MD-predicted consequences of the effects of the DMSO at low and intermediate DMSO concentrations. At larger DMSO concentrations, permeabilization is larger, as even Yo-Pro-1 can enter the cells as predicted by the DMSO-induced membrane-destructuring effects described in the MD simulations. © 2012 de Ménorval et al.

Descripción: To understand how the Rhizobium leguminosarum rail-raiR quorum-sensing system is regulated, we identified mutants with decreased levels of RaiI-made N-acyl homoserine lactones (AHLs). A LuxR-type regulator, ExpR, is required for raiR expression, and RaiR is required to induce rail. Since raiR (and rail) expression is also reduced in cinI and cinR quorum-sensing mutants, we thought CinI-made AHLs may activate ExpR to induce raiR. However, added CinI-made AHLs did not induce raiR expression in a cinI mutant. The reduced raiR expression in cinI and cinR mutants was due to lack of expression of cinS immediately downstream of cinI. cinS encodes a 67-residue protein, translationally coupled to CinI, and cinS acts downstream of expR for raiR induction. Cloned cinS in R. leguminosarum caused an unusual collapse of colony structure, and this was delayed by mutation of expR. The phenotype looked like a loss of exopolysaccharide (EPS) integrity; mutations in cinI, cinR, cinS, and expR all reduced expression of plyB, encoding an EPS glycanase, and mutation of plyB abolished the effect of cloned cinS on colony morphology. We conclude that CinS and ExpR act to increase PlyB levels, thereby influencing the bacterial surface. CinS is conserved in other rhizobia, including Rhizobium etli; the previously observed effect of cinI and cinR mutations decreasing swarming in that strain is primarily due to a lack of CinS rather than a lack of CinI-made AHL. We conclude that CinS mediates quorum-sensing regulation because it is coregulated with an AHL synthase and demonstrate that its regulatory effects can occur in the absence of AHLs. Copyright © 2009, American Society for Microbiology.

Descripción: In the region of the Main Andes of San Juan, exists a high concentration of landslide deposits that can originate natural dams. The outburst of these dams generated by slide is a common process in this morphology. The analyses of satellite imagery and air photos of previous years allow reconstructing the characteristics and the origin of the slides that formed those natural dams. In this region of the Main Andes, the rapid uplift and erosion of the mountain chain has created abrupt slopes and an internal structure in the materials that determined an increase of the susceptibility to the slope collapse without triggers as the seismic activity or precipitations. The objectives of the present work are (1) to analyze the causes that formed the natural dam and produced the landslide dam and development of the Los Erizos lagoon in the middle course of the Santa Cruz river; (2) the outburst of the dam and their consequent flood; and (3) the morphologic characteristic of the study region. The temporary comparison between air photos with satellite imageries indicates that the natural dam was generated by slip of the western hillside of the Santa Cruz mountain range, located below the Cerro Estrella. However, the constant water flow to the lagoon, has increased its water level until reaching the maximum benchmark of the dam, produced the beginning of the lake drainage and weakening of the dam causing the outburst and its consequent flood. Based on satellite imagery, a temporary analysis of the lagoon is presented, indicating that monitoring is an important tool to alert in the whole San Juan river basin for possible natural dikes, preventing hazards to the populations down water. The alluvium of November 12 of 2005, discharged 32.100.000 m3 in 67 minutes and traveled 254 km in less than 12 hours.