Descripción: Correlation anisotropy emerges dynamically in magnetohydrodynamics (MHD), producing stronger gradients across the large-scale mean magnetic field than along it. This occurs both globally and locally, and has significant implications in space and astrophysical plasmas, including particle scattering and transport, and theories of turbulence. Properties of local correlation anisotropy are further documented here by showing through numerical experiments that the effect is intensified in more localized estimates of the mean field. The mathematical formulation of this property shows that local anisotropy mixes second-order with higher order correlations. Sensitivity of local statistical estimates to higher order correlations can be understood in connection with the stochastic coordinate system inherent in such formulations. We demonstrate this in specific cases, and illustrate the connection to higher order statistics by showing the sensitivity of local anisotropy to phase randomization, after which the global measure of anisotropy is recovered at all scales of averaging. This establishes that anisotropy of the local structure function is not a measure of anisotropy of the energy spectrum. Evidently, the local enhancement of correlation anisotropy is of substantial fundamental interest and must be understood in terms of higher order correlations, specifically fourth-order and above. © 2012. The American Astronomical Society. All rights reserved.

Descripción: This paper describes a matrix formulation for the correlated hole theory within the framework of the domain-averaged model in many electron systems (atoms, molecules, condensed matter, etc.). General relationships between this quantity and one-particle reduced density matrices for any independent particle or correlated state functions are presented. This formulation turns out to be suitable for computational purposes due to the straightforward introduction of cumulants of two-particle reduced density matrices within the quantum field structure. Numerical calculations in selected simple molecular systems have been performed in order to determine preliminary correlated values for such a quantity.

Descripción: In this paper we compare two methods, based on the Gabor and wavelet transforms, to quantify and visualize the time evolution of frequency contents of electroencephalogram (EEG) time series. We found an optimal correlation between EEG visual inspection and the proposed methods in the characterization of the frequency and energy content of characteristic activity during an epileptic seizure. The quasimonofrequency behavior observed in the epileptic EEG series, in a previous work using a Gabor analysis [J. Inst. Electr. Eng. 93, 429 (1946)], is confirmed with the analysis using a wavelet. Moreover, the method based on the wavelet transform allows us to build a detector of epileptic events. Both methods are exemplified with EEG series obtained with depth electrodes in refractory epileptic patients. © 1996 The American Physical Society.

Descripción: Aims: To evaluate the antagonistic activity of Fusarium oxysporum nonpathogenic fungal strain S6 against the phytopathogenic fungus Sclerotinia sclerotiorum and to identify the antifungal compounds involved. Methods and Results: The antagonistic activity of Fusarium oxysporum strain S6 was determined in vitro by dual cultures. The metabolite responsible for the activity was isolated by chromatographic techniques, purified and identified by spectroscopic methods as cyclosporine A. The antifungal activity against the pathogen was correlated with the presence of this metabolite by a dilution assay and then quantified. Cyclosporine A caused both growth inhibition and suppression of sclerotia formation. In a greenhouse assay, a significant increase in the number of surviving soybean (Glycine max) plants was observed when S. sclerotiorum and F. oxysporum (S6) were inoculated together when compared with plants inoculated with S. sclerotiorum alone. Conclusion: Fusarium oxysporum (S6) may be a good fungal biological control agent for S. sclerotiorum and cyclosporine A is the responsible metabolite involved in its antagonistic activity in vitro. Significance and Impact of the Study: Cyclosporine A has not been previously described as an inhibitor of S. sclerotiorum. Its minimum inhibitory concentration (MIC) of 0·1 μg disc-1 makes it suitable to use as a biofungicide. In vivo experiments showed that F. oxysporum (S6) is a good candidate for the biocontrol of S. sclerotiorum in soybean. © 2006 The Society for Applied Microbiology.

Descripción: We present a parametric space study of the decay of turbulence in rotating flows combining direct numerical simulations, large eddy simulations, and phenomenological theory. Several cases are considered: (1) the effect of varying the characteristic scale of the initial conditions when compared with the size of the box, to mimic "bounded" and "unbounded" flows; (2) the effect of helicity (correlation between the velocity and vorticity); (3) the effect of Rossby and Reynolds numbers; and (4) the effect of anisotropy in the initial conditions. Initial conditions include the Taylor-Green vortex, the Arn'old-Beltrami-Childress flow, and random flows with large-scale energy spectrum proportional to k4. The decay laws obtained in the simulations for the energy, helicity, and enstrophy in each case can be explained with phenomenological arguments that consider separate decays for two-dimensional and three-dimensional modes and that take into account the role of helicity and rotation in slowing down the energy decay. The time evolution of the energy spectrum and development of anisotropies in the simulations are also discussed. Finally, the effect of rotation and helicity in the skewness and kurtosis of the flow is considered. © 2011 American Institute of Physics.

Descripción: An experimental investigation was performed to study the effect on the free volume of the advance of the cross-linking reaction in a copolymer of styrene butadiene rubber by sulfur vulcanization. The dynamic modulus and loss tangent were evaluated over samples cured for different times at 433 K by dynamic mechanical tests over a range of frequencies between 5 and 80 Hz at temperatures between 200 and 300 K. Using the William-Landel-Ferry relationship, master curves were obtained at a reference temperature of 298 K and the coefficients [formula presented] and [formula presented] were evaluated. From these parameters the dependence of the free volume on the cure time is obtained. Positron annihilation lifetime spectroscopy was also used to estimate the size and number density of free volume sites in the material. The spectra were analyzed in terms of continuous distributions of free volume size. The results suggest an increase of the lower free volume size when cross linking takes place. Both techniques give similar results for the dependence of free volume on the time of cure of the polymer. © 2002 The American Physical Society.

Descripción: Context. Coronal mass ejections (CMEs) are routinely tracked with imagers in the interplanetary space, while magnetic clouds (MCs) properties are measured locally by spacecraft. However, both imager and in situ data do not provide any direct estimation of the general flux rope properties. Aims. The main aim of this study is to constrain the global shape of the flux rope axis from local measurements and to compare the results from in-situ data with imager observations. Methods. We performed a statistical analysis of the set of MCs observed by WIND spacecraft over 15 years in the vicinity of Earth. We analyzed the correlation between different MC parameters and studied the statistical distributions of the angles defining the local axis orientation. With the hypothesis of having a sample of MCs with a uniform distribution of spacecraft crossing along their axis, we show that a mean axis shape can be derived from the distribution of the axis orientation. As a complement, while heliospheric imagers do not typically observe MCs but only their sheath region, we analyze one event where the flux rope axis can be estimated from the STEREO imagers. Results. From the analysis of a set of theoretical models, we show that the distribution of the local axis orientation is strongly affected by the overall axis shape. Next, we derive the mean axis shape from the integration of the observed orientation distribution. This shape is robust because it is mostly determined from the overall shape of the distribution. Moreover, we find no dependence on the flux rope inclination on the ecliptic. Finally, the derived shape is fully consistent with the one derived from heliospheric imager observations of the June 2008 event. Conclusions. We have derived a mean shape of MC axis that only depends on one free parameter, the angular separation of the legs (as viewed from the Sun). This mean shape can be used in various contexts, such as studies of high-energy particles or space weather forecasts. © ESO, 2013.

Descripción: Background: Detailed analysis of the dynamic interactions among biological, environmental, social, and economic factors that favour the spread of certain diseases is extremely useful for designing effective control strategies. Diseases like tuberculosis that kills somebody every 15 seconds in the world, require methods that take into account the disease dynamics to design truly efficient control and surveillance strategies. The usual and well established statistical approaches provide insights into the cause-effect relationships that favour disease transmission but they only estimate risk areas, spatial or temporal trends. Here we introduce a novel approach that allows figuring out the dynamical behaviour of the disease spreading. This information can subsequently be used to validate mathematical models of the dissemination process from which the underlying mechanisms that are responsible for this spreading could be inferred. Methodology/Principal Findings: The method presented here is based on the analysis of the spread of tuberculosis in a Brazilian endemic city during five consecutive years. The detailed analysis of the spatio-temporal correlation of the yearly geo-referenced data, using different characteristic times of the disease evolution, allowed us to trace the temporal path of the aetiological agent, to locate the sources of infection, and to characterize the dynamics of disease spreading. Consequently, the method also allowed for the identification of socio-economic factors that influence the process. Conclusions/Significance: The information obtained can contribute to more effective budget allocation, drug distribution and recruitment of human skilled resources, as well as guiding the design of vaccination programs. We propose that this novel strategy can also be applied to the evaluation of other diseases as well as other social processes. © 2010 Zorzenon dos Santos et al.

Descripción: We investigate the importance of relativistic effects on NMR shielding constants and chemical shifts of linear HgL2 (L = Cl, Br, I, CH 3) compounds using three different relativistic methods: the fully relativistic four-component approach and the two-component approximations, linear response elimination of small component (LR-ESC) and zeroth-order regular approximation (ZORA). LR-ESC reproduces successfully the four-component results for the C shielding constant in Hg(CH3)2 within 6 ppm, but fails to reproduce the Hg shielding constants and chemical shifts. The latter is mainly due to an underestimation of the change in spin-orbit contribution. Even though ZORA underestimates the absolute Hg NMR shielding constants by ∼2100 ppm, the differences between Hg chemical shift values obtained using ZORA and the four-component approach without spin-density contribution to the exchange-correlation (XC) kernel are less than 60 ppm for all compounds using three different functionals, BP86, B3LYP, and PBE0. However, larger deviations (up to 366 ppm) occur for Hg chemical shifts in HgBr 2 and HgI2 when ZORA results are compared with four-component calculations with non-collinear spin-density contribution to the XC kernel. For the ZORA calculations it is necessary to use large basis sets (QZ4P) and the TZ2P basis set may give errors of ∼500 ppm for the Hg chemical shifts, despite deceivingly good agreement with experimental data. A Gaussian nucleus model for the Coulomb potential reduces the Hg shielding constants by ∼100-500 ppm and the Hg chemical shifts by 1-143 ppm compared to the point nucleus model depending on the atomic number Z of the coordinating atom and the level of theory. The effect on the shielding constants of the lighter nuclei (C, Cl, Br, I) is, however, negligible. © 2011 American Institute of Physics.

Descripción: The subcellular localization and physiological functions of biomolecules are closely related and thus it is crucial to precisely determine the distribution of different molecules inside the intracellular structures. This is frequently accomplished by fluorescence microscopy with well-characterized markers and posterior evaluation of the signal colocalization. Rigorous study of colocalization requires statistical analysis of the data, albeit yet no single technique has been established as a standard method. Indeed, the few methods currently available are only accurate in images with particular characteristics. Here, we introduce a new algorithm to automatically obtain the true colocalization between images that is suitable for a wide variety of biological situations. To proceed, the algorithm contemplates the individual contribution of each pixel's fluorescence intensity in a pair of images to the overall Pearsońs correlation and Manders' overlap coefficients. The accuracy and reliability of the algorithm was validated on both simulated and real images that reflected the characteristics of a range of biological samples. We used this algorithm in combination with image restoration by deconvolution and time-lapse confocal microscopy to address the localization of MEK1 in the mitochondria of different cell lines. Appraising the previously described behavior of Akt1 corroborated the reliability of the combined use of these techniques. Together, the present work provides a novel statistical approach to accurately and reliably determine the colocalization in a variety of biological images. © 2011 Villalta et al.

Descripción: Background: Sixteen melanoma patients (1 stage IIC, 8 stage III, and 7 stage IV) were treated in a Phase I study with a vaccine (DC/Apo-Nec) composed of autologous dendritic cells (DCs) loaded with a mixture of apoptotic/necrotic allogeneic melanoma cell lines (Apo-Nec), to evaluate toxicity and immune responses. Also, IL-10 1082 genotype was analyzed in an effort to predict disease progression. Methods: PBMC were obtained after leukapheresis and DCs were generated from monocytes cultured in the presence of GM-CSF and IL-4 in serum-free medium. Immature DCs were loaded with gamma-irradiated Apo-Nec cells and injected id without adjuvant. Cohorts of four patients were given four vaccines each with 5, 10, 15, or 20 × 106 DC/Apo-Nec cell per vaccine, two weeks apart. Immune responses were measured by ELISpot and tetramer analysis. Il-10 genotype was measured by PCR and corroborated by IL-10 production by stimulated PBMC. Results: Immature DCs efficiently phagocytosed melanoma Apo-Nec cells and matured after phagocytosis as evidenced by increased expression of CD83, CD80, CD86, HLA class I and II, and 75.2 ± 16% reduction in Dextran-FITC endocytosis. CCR7 was also up-regulated upon Apo-Nec uptake in DCs from all patients, and accordingly DC/Apo-Nec cells were able to migrate in vitro toward MIP-3 beta. The vaccine was well tolerated in all patients. The DTH score increased significantly in all patients after the first vaccination (Mann-Whitney Test, p < 0.05). The presence of CD8+T lymphocytes specific to gp100 and Melan A/ MART-1 Ags was determined by ELISpot and tetramer analysis in five HLA-A*0201 patients before and after vaccination; one patient had stable elevated levels before and after vaccination; two increased their CD8 + levels, one had stable moderate and one had negligible levels. The analysis of IL-10 promoter -1082 polymorphism in the sixteen patients showed a positive correlation between AA genotype, accompanied by lower in vitro IL-10 production by stimulated PBMC, and faster melanoma progression after lymph nodes surgery (p = 0.04). With a mean follow-up of 49.5 months post-surgery, one stage IIC patient and 7/8 stage III patients remain NED but 7/7 stage IV patients have progressed. Conclusion: We conclude that DC/Apo-Nec vaccine is safe, well tolerated and it may induce specific immunity against melanoma Ags. Patients with a low-producing IL-10 polymorphism appear to have a worst prognosis. © 2008 von Euw et al; licensee BioMed Central Ltd.

Descripción: Aims.We investigate the morphology at low radio frequencies of the supernova remnant (SNR) IC 443 in detail and accurately establish its radio continuum spectral properties. Methods. We used the VLA in multiple configurations to produce high-resolution radio images of IC 443 at 74 and 330 MHz. From these data we produced the first sensitive, spatially resolved spectral analysis of the radio emission at long wavelengths. The changes with position in the radio spectral index were correlated with data in near infrared (NIR) from 2MASS, in gamma-rays from VERITAS, and with the molecular 12CO (J = 1-0) line emission. Results. The new image at 74 MHz has HPBW = 35′′ and rms = 30 mJy beam-1 and at 330 MHz HPBW = 17′′and rms = 1.7 mJy beam-1. The integrated flux densities for the whole SNR are S SNR 74MHz = 470 ± 51 Jy and S SNR 330MHz = 248 ± 15 Jy. Improved estimates of the integrated spectrum were derived taking a turnover into account to fit the lowest frequency measurements in the literature. Combining our measurements with existing data, we derive an integrated spectral index α 10700MHz 10MHz =-0.39 ± 0.01 with a free-free continuum optical depth at 330 MHz 330 ∼ 7 × 10 -4 (τ10 = 1.07); all measurements above ∼10 MHz are equally consistent with a power law spectrum. For the pulsar wind nebula associated with the compact source CXOU J061705.3+222127, we calculated S PWN 330MHz = 0.23±0.05 Jy, SPWN 1420MHz = 0.20±0.04 Jy, and α8460MHz 330MHz ∼ 0.0. Substantial variations are observed in spectral index between 74 and 330 MHz across IC 443. The flattest spectral components (-0.25 = a =-0.05) coincide with the brightest parts of the SNR along the eastern border, with an impressive agreement with ionic lines as observed in the 2MASS J and H bands. The diffuse interior of IC 443 has a spectrum steeper than found anywhere in the SNR (-0.85 = a =-0.6), while the southern ridge again has a flatter spectrum (a ∼-0.4). With the available statistics the VERITAS .-ray emission strikingly matches the CO distribution, but no clear evidence is found for a morphological correlation between the TeV distribution and radio emission. Conclusions. The excellent correspondence between the eastern radio flattest spectrum region and NIR ionic lines strongly suggests that the passage of a fast, dissociating J-type shock across the interacting molecular cloud dissociated the molecules and ionized the gas.We therefore conclude that thermal absorption at 74 MHz (τ74 up to ∼0.3) is responsible for the localized spectral index flattening observed along the eastern border of IC 443. Towards the interior of IC 443, the spectrum is consistent with those expected from linear diffusive shock acceleration, while the flatter spectrum in the southern ridge is a consequence of the strong shock/molecular cloud interaction. © 2011 ESO.

Descripción: Context. Recent observational and theoretical works have suggested that the Tully-Fisher relation might be generalised to include dispersion-dominated systems by combining the rotation and dispersion velocity in the definition of the kinematical indicator. Mergers and interactions have been pointed out as responsible of driving turbulent and disordered gas kinematics, which could generate Tully-Fisher relation outliers. Aims. We investigated the gas kinematics of galaxies by using a simulated sample that includes gas-disc-dominated as well as spheroid-dominated systems. We paid particular attention to the scatter evolution of the Tully-Fisher relation. We also determined the gas-phase velocity indicator, which traces the potential well of the galaxy better. Methods. Cosmological hydrodynamical simulations that include a multiphase model and physically motivated supernova feedback were performed to follow the evolution of galaxies as they are assembled. We analysed the gas kinematics of the surviving gas discs to estimate all velocity indicators. Results. Both the baryonic and stellar Tully-Fisher relations for gas-disc-dominated systems are tight while, as more dispersion-dominated systems are included, the scatter increases. We found a clear correlation between σ/V rot and morphology, with dispersion-dominated systems exhibiting higher values (>0.7). Mergers and interactions can affect the rotation curves directly or indirectly, inducing a scatter in the Tully-Fisher relation larger than the simulated evolution since z ~ 3. Kinematical indicators, which combine rotation velocity and dispersion velocity, can reduce the scatter in the baryonic and the stellar mass-velocity relations. In particular, s 1.0 = (V rot 2 + σ 2) 0.5 seems to be the best tracer of the circular velocity at larger radii. Our findings also show that the lowest scatter in both relations is obtained if the velocity indicators are measured at the maximum of the rotation curve. Conclusions. In agreement with previous works, we found that the gas kinematics of galaxies is significantly regulated by mergers and interactions, which play a key role in inducing gas accretion, outflows and starbursts. The joint action of these processes within a hierarchical ΛCDM Universe generates a mean simulated Tully-Fisher relation in good agreement with observations since z ~ 3 but with a scatter depending on morphology. The rotation velocity estimated at the maximum of the gas rotation curve is found to be the best proxy for the potential well regardless of morphology. ©2012 ESO.