Descripción: An experimental validation of an model for the production of birdsongs was discussed. The derivation of the model with functions whose time dependence came from recordings of muscle activities and air sac pressure was studied. It was found that birds' songs were recorded and compared with the synthetic songs. Some predictions of the model concerning the relative levels of activity in the gating muscles at the beginning and end of a syllable were also tested.

Descripción: The recent solar minimum with very low activity provides us a unique opportunity for validating solar wind models. During CR2077 (2008 November 20 through December 17), the number of sunspots was near the absolute minimum of solar cycle 23. For this solar rotation, we perform a multi-spacecraft validation study for the recently developed three-dimensional, two-temperature, Alfvén-wave-driven global solar wind model (a component within the Space Weather Modeling Framework). By using in situ observations from the Solar Terrestrial Relations Observatory (STEREO) A and B, Advanced Composition Explorer (ACE), and Venus Express, we compare the observed proton state (density, temperature, and velocity) and magnetic field of the heliosphere with that predicted by the model. Near the Sun, we validate the numerical model with the electron density obtained from the solar rotational tomography of Solar and Heliospheric Observatory/Large Angle and Spectrometric Coronagraph C2 data in the range of 2.4 to 6 solar radii. Electron temperature and density are determined from differential emission measure tomography (DEMT) of STEREO A and B Extreme Ultraviolet Imager data in the range of 1.035 to 1.225 solar radii. The electron density and temperature derived from the Hinode/Extreme Ultraviolet Imaging Spectrometer data are also used to compare with the DEMT as well as the model output. Moreover, for the first time, we compare ionic charge states of carbon, oxygen, silicon, and iron observed in situ with the ACE/Solar Wind Ion Composition Spectrometer with those predicted by our model. The validation results suggest that most of the model outputs for CR2077 can fit the observations very well. Based on this encouraging result, we therefore expect great improvement for the future modeling of coronal mass ejections (CMEs) and CME-driven shocks. © 2012. The American Astronomical Society. All rights reserved.

Descripción: Daily station precipitation totals are used to develop a gridded dataset for the region (14°-40°S, 45°-70°W) on a 0.5° × 0.5° latitude/longitude grid, primarily for comparison with regional climate model (RCM) simulations. The gridded dataset covers the period 1961-2000. Much of the paper discusses the quality control of the basic station precipitation series. Although the primary aim of the development has been RCM validation, we have assessed trends in seasonal precipitation totals as well as trends in two measures of precipitation extremes (R95p, the daily precipitation amount exceeded only 5% of the time and Rx5day, the maximum 5-d precipitation total during each season). Relatively few regions across the large domain have statistically significant trends, but those that do tend to be located in the eastern two thirds of the grid, particularly over southeastern Brazil and Uruguay. Significant trends are also more evident in the DJF and MAM seasons. There is good spatial agreement between the trends in seasonal totals and trends in the extreme indices. © 2012 Royal Meteorological Society.

Descripción: This paper evaluates a present climate simulation over southern South America performed with the Meteorological Research Institute/Japanese Meteorological Agency (MRI/JMA) high resolution global model. Main patterns of low and upper-level atmospheric circulation were analysed in a 25-year simulation as well as climate means, interannual variability, trends and bias of temperature and precipitation. The model is able to reproduce the main atmospheric circulation patterns and mean surface variables, although some deficiencies were found such as an overestimation of temperature over central Argentina in most of seasons, an overestimation of austral winter precipitation over northeastern and central Argentina, an underestimation of precipitation in all the seasons over southeast of Brazil, an underestimation of the amplitude of the annual cycle temperature in some regions and an overestimation of the amplitude of the annual cycle of precipitation over mountainous areas. Model interannual variability was also assessed. In general, temperature variability is overestimated, whereas precipitation is underestimated. The spatial structure of the year-to-year variability of precipitation is correctly simulated by the model, although some patterns were misplaced. Most of regions present a cold seasonal bias reaching values of - 2 °C in some regions. It was found that precipitation biases are between 3 and - 1 mm day-1. In some regions and seasons observed and simulated temperature trends coincide, as in austral summer or spring, where the model and the observations show positive trends in most of regions. However, there is no agreement between observed and simulated precipitation trends in almost all the regions and seasons. © 2012 Royal Meteorological Society.

Descripción: Oligonucleotide-based microarrays with accurate gene coverage represent a key strategy for transcriptional studies in orphan species such as sunflower, H. annuus L., which lacks full genome sequences. The goal of this study was the development and functional annotation of a comprehensive sunflower unigene collection and the design and validation of a custom sunflower oligonucleotide-based microarray. A large scale EST (>130,000 ESTs) curation, assembly and sequence annotation was performed using Blast2GO (www.blast2go.de). The EST assembly comprises 41,013 putative transcripts (12,924 contigs and 28,089 singletons). The resulting Sunflower Unigen Resource (SUR version 1.0) was used to design an oligonucleotide-based Agilent microarray for cultivated sunflower. This microarray includes a total of 42,326 features: 1,417 Agilent controls, 74 control probes for sunflower replicated 10 times (740 controls) and 40,169 different non-control probes. Microarray performance was validated using a model experiment examining the induction of senescence by water deficit. Pre-processing and differential expression analysis of Agilent microarrays was performed using the Bioconductor limma package. The analyses based on p-values calculated by eBayes (p<0.01) allowed the detection of 558 differentially expressed genes between water stress and control conditions; from these, ten genes were further validated by qPCR. Over-represented ontologies were identified using FatiScan in the Babelomics suite. This work generated a curated and trustable sunflower unigene collection, and a custom, validated sunflower oligonucleotide-based microarray using Agilent technology. Both the curated unigene collection and the validated oligonucleotide microarray provide key resources for sunflower genome analysis, transcriptional studies, and molecular breeding for crop improvement. © 2012 Fernandez et al.

Descripción: Background: Prosopis alba (Fabaceae) is an important native tree adapted to arid and semiarid regions of north-western Argentina which is of great value as multipurpose species. Despite its importance, the genomic resources currently available for the entire Prosopis genus are still limited. Here we describe the development of a leaf transcriptome and the identification of new molecular markers that could support functional genetic studies in natural and domesticated populations of this genus.Results: Next generation DNA pyrosequencing technology applied to P. alba transcripts produced a total of 1,103,231 raw reads with an average length of 421 bp. De novo assembling generated a set of 15,814 isotigs and 71,101 non-assembled sequences (singletons) with an average of 991 bp and 288 bp respectively. A total of 39,000 unique singletons were identified after clustering natural and artificial duplicates from pyrosequencing reads.Regarding the non-redundant sequences or unigenes, 22,095 out of 54,814 were successfully annotated with Gene Ontology terms. Moreover, simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs) were searched, resulting in 5,992 and 6,236 markers, respectively, throughout the genome. For the validation of the the predicted SSR markers, a subset of 87 SSRs selected through functional annotation evidence was successfully amplified from six DNA samples of seedlings. From this analysis, 11 of these 87 SSRs were identified as polymorphic. Additionally, another set of 123 nuclear polymorphic SSRs were determined in silico, of which 50% have the probability of being effectively polymorphic.Conclusions: This study generated a successful global analysis of the P. alba leaf transcriptome after bioinformatic and wet laboratory validations of RNA-Seq data.The limited set of molecular markers currently available will be significantly increased with the thousands of new markers that were identified in this study. This information will strongly contribute to genomics resources for P. alba functional analysis and genetics. Finally, it will also potentially contribute to the development of population-based genome studies in the genera. © 2013 Torales et al.; licensee BioMed Central Ltd.

Descripción: Trypanosoma cruzi, the aetiological agent of Chagas' disease, is exposed to extremely different environment conditions during its life cycle, and transporters are key molecules for its adaptive regulation. Amino acids, and particularly arginine, are essential components in T. cruzi metabolism. In this work, a novel T. cruzi arginine permease was identified by screening different members of the AAAP family (amino acid/auxin permeases) in yeast complementation assays using a toxic arginine analogue. One gene candidate, TcAAAP411, was characterized as a very specific, high-affinity, l-arginine permease. This work is the first identification of the molecular components involved specifically in amino acid transport in T. cruzi and provides new insights for further validation of the TcAAAP family as functional permeases. © 2010 Federation of European Microbiological Societies.

Descripción: [i] This work presents results, over the South American region, from the CSIRO Division of Atmospheric Research limited area model (DARLAM) and from the ninelevel general circulation model (GCM) of CSIRO (CSIRO Mk 2), within which it was nested in a one-way mode. Ten separate 30-day DARLAM simulations were performed for both January and July with a resolution of 125 km and were averaged to obtain a climatology for those months. This paper presents for the first time simulations of the January South American climate using a limited area model; previous similar studies simulated only the July climate. The goal of this study was to examine the capability of the CSIRO Mk 2 - DARLAM nested modeling system for simulating the climate in the South American region. Further, it was desired to investigate whether the higher resolution of DARLAM improves the simulated climate over various subregions, compared with the GCM and observations. With this purpose, a representative set of variables was analyzed and statistically compared. Overall, the fields simulated by the nesting system provide a better representation of the South American climate than the GCM. In particular, significant improvements are found in the nested model climatology for near-surface temperature and mean sea level pressure. Comparison of the January and July simulations shows a better wintertime performance. Some significant summertime features, like the Bolivian High, are reasonably well simulated by DARLAM, but not by the GCM. Copyright 2002 by the American Geophysical Union.

Descripción: The rise in the world demand for food poses a challenge to our ability to sustain soil fertility and sustainability. The increasing use of no-till agriculture, adopted in many areas of the world as an alternative to conventional farming, may contribute to reduce the erosion of soils and the increase in the soil carbon pool. However, the advantages of no-till agriculture are jeopardized when its use is linked to the expansion of crop monoculture. The aim of this study was to survey bacterial communities to find indicators of soil quality related to contrasting agriculture management in soils under no-till farming. Four sites in production agriculture, with different soil properties, situated across a west-east transect in the most productive region in the Argentinean pampas, were taken as the basis for replication. Working definitions of Good no-till Agricultural Practices (GAP) and Poor no-till Agricultural Practices (PAP) were adopted for two distinct scenarios in terms of crop rotation, fertilization, agrochemicals use and pest control. Non-cultivated soils nearby the agricultural sites were taken as additional control treatments. Tag-encoded pyrosequencing was used to deeply sample the 16S rRNA gene from bacteria residing in soils corresponding to the three treatments at the four locations. Although bacterial communities as a whole appeared to be structured chiefly by a marked biogeographic provincialism, the distribution of a few taxa was shaped as well by environmental conditions related to agricultural management practices. A statistically supported approach was used to define candidates for management-indicator organisms, subsequently validated using quantitative PCR. We suggest that the ratio between the normalized abundance of a selected group of bacteria within the GP1 group of the phylum Acidobacteria and the genus Rubellimicrobium of the Alphaproteobacteria may serve as a potential management-indicator to discriminate between sustainable vs. non-sustainable agricultural practices in the Pampa region. © 2012 Figuerola et al.