4 documentos corresponden a la consulta.
Palabras contadas: puffs: 11
Solovey, G. - Fraiman, D. - Dawson, S.P.
Front. Physiol. 2011;2 AUG
2011
Descripción: Mean field models are often useful approximations to biological systems, but sometimes, they can yield misleading results. In this work, we compare mean field approaches with stochastic models of intracellular calcium release. In particular, we concentrate on calcium signals generated by the concerted opening of several clustered channels (calcium puffs). To this end we simulate calcium puffs numerically and then try to reproduce features of the resulting calcium distribution using mean field models were all the channels open and close simultaneously. We show that an unrealistic non-linear relationship between the current and the number of open channels is needed to reproduce the simulated puffs. Furthermore, a single channel current which is five times smaller than the one of the stochastic simulations is also needed. Our study sheds light on the importance of the stochastic kinetics of the calcium release channel activity to estimate the release fluxes. © 2011 Solovey, Fraiman and Dawson.
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Fraiman, D. - Pando, B. - Dargan, S. - Parker, I. - Dawson, S.P.
Biophys. J. 2006;90(11):3897-3907
2006
Temas: 1,4,5 triphosphate - calcium - phosphate - unclassified drug - animal cell - article - controlled study - dynamics - endoplasmic reticulum - fluorescence
Descripción: Puffs are localized Ca2+ signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP3). They are analogous to the sparks of myocytes and are believed to be the result of the liberation of Ca2+ from the endoplasmic reticulum through the coordinated opening of IP3 receptor/channels clustered at a functional release site. In this article, we analyze sequences of puffs that occur at the same site to help elucidate the mechanisms underlying puff dynamics. In particular, we show a dependence of the interpuff time on the amplitude of the preceding puff, and of the amplitude of the following puff on the preceding interval. These relationships can be accounted for by an inhibitory role of the Ca2+ that is liberated during puffs. We construct a stochastic model for a cluster of IP3 receptor/channels that quantitatively replicates the observed behavior, and we determine that the characteristic time for a channel to escape from the inhibitory state is of the order of seconds. © 2006 by the Biophysical Society.
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Solovey, G. - Dawson, S.P.
PLoS ONE 2010;5(2)
2010
Temas: calcium channel - calcium ion - inositol 1,4,5 trisphosphate receptor - calcium - calcium channel - inositol 1,4,5 trisphosphate - inositol 1,4,5 trisphosphate receptor - article - binding competition - calcium signaling
Descripción: Calcium signals are involved in a large variety of physiological processes. Their versatility relies on the diversity of spatiotemporal behaviors that the calcium concentration can display. Calcium entry through inositol 1,4,5-trisphosphate (IP3) receptors (IP3R's) is a key component that participates in both local signals such as "puffs" and in global waves. IP3R's are usually organized in clusters on the membrane of the endoplasmic reticulum and their spatial distribution has important effects on the resulting signal. Recent high resolution observations [1] of Ca2+ puffs offer a window to study intra-cluster organization. The experiments give the distribution of the number of IP3R's that open during each puff without much processing. Here we present a simple model with which we interpret the experimental distribution in terms of two stochastic processes: IP3 binding and unbinding and Ca2+-mediated inter-channel coupling. Depending on the parameters of the system, the distribution may be dominated by one or the other process. The transition between both extreme cases is similar to a percolation process. We show how, from an analysis of the experimental distribution, information can be obtained on the relative weight of the two processes. The largest distance over which Ca2+mediated coupling acts and the density of IP3-bound IP3R's of the cluster can also be estimated. The approach allows us to infer properties of the interactions among the channels of the cluster from statistical information on their emergent collective behavior. © 2010 Solovey, Dawson.
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Solovey, G. - Dawson, S.P.
Philos. Trans. R. Soc. A Math. Phys. Eng. Sci. 2010;368(1933):5597-5603
2010
Temas: Buffers - Puffs - Sugars - Transport properties - Buffers - High resolution - Local signal - Optical experiments - Physiological process - Puffs
Descripción: Calcium signals participate in a large variety of physiological processes. In many instances, they involve calcium entry through inositol 1,4,5-trisphosphate (IP3) receptors (IP3Rs), which are usually organized in clusters. Recent high-resolution optical experiments by Smith & Parker have provided new information on Ca2+ release from clustered IP3Rs. In the present paper, we use the model recently introduced by Solovey & Ponce Dawson to determine how the distribution of the number of IP3Rs that become open during a localized release event may change by the presence of Ca2+ buffers, substances that react with Ca2+, altering its concentration and transport properties. We then discuss how buffer properties could be extracted from the observation of local signals. © 2010 The Royal Society.
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