2 documentos corresponden a la consulta.
Palabras contadas: hemoglobin: 14, truncated: 14
Oliveira, A. - Singh, S. - Bidon-Chanal, A. - Forti, F. - Martí, M.A. - Boechi, L. - Estrin, D.A. - Dikshit, K.L. - Luque, F.J.
PLoS ONE 2012;7(11)
2012
Temas: alanine - dioxygenase - heme - isoleucine - nitric oxide dioxygenase - oxygen - phenylalanine - phenylalanine 15 - truncated hemoglobin - truncated hemoglobin N
Descripción: The truncated hemoglobin N, HbN, of Mycobacterium tuberculosis is endowed with a potent nitric oxide dioxygenase (NOD) activity that allows it to relieve nitrosative stress and enhance in vivo survival of its host. Despite its small size, the protein matrix of HbN hosts a two-branched tunnel, consisting of orthogonal short and long channels, that connects the heme active site to the protein surface. A novel dual-path mechanism has been suggested to drive migration of O 2 and NO to the distal heme cavity. While oxygen migrates mainly by the short path, a ligand-induced conformational change regulates opening of the long tunnel branch for NO, via a phenylalanine (PheE15) residue that acts as a gate. Site-directed mutagenesis and molecular simulations have been used to examine the gating role played by PheE15 in modulating the NOD function of HbN. Mutants carrying replacement of PheE15 with alanine, isoleucine, tyrosine and tryptophan have similar O 2 /CO association kinetics, but display significant reduction in their NOD function. Molecular simulations substantiated that mutation at the PheE15 gate confers significant changes in the long tunnel, and therefore may affect the migration of ligands. These results support the pivotal role of PheE15 gate in modulating the diffusion of NO via the long tunnel branch in the oxygenated protein, and hence the NOD function of HbN. © 2012 Oliveira et al.
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Marcelli, A. - Abbruzzetti, S. - Bustamante, J.P. - Feis, A. - Bonamore, A. - Boffi, A. - Gellini, C. - Salvi, P.R. - Estrin, D.A. - Bruno, S. - Viappiani, C. - Foggi, P.
PLoS ONE 2012;7(7)
2012
Temas: carbon monoxide - truncated hemoglobin - article - bacterium - binding affinity - binding kinetics - binding site - complex formation - controlled study - enthalpy
Descripción: CO recombination kinetics has been investigated in the type II truncated hemoglobin from Thermobifida fusca (Tf-trHb) over more than 10 time decades (from 1 ps to ~100 ms) by combining femtosecond transient absorption, nanosecond laser flash photolysis and optoacoustic spectroscopy. Photolysis is followed by a rapid geminate recombination with a time constant of ~2 ns representing almost 60% of the overall reaction. An additional, small amplitude geminate recombination was identified at ~100 ns. Finally, CO pressure dependent measurements brought out the presence of two transient species in the second order rebinding phase, with time constants ranging from ~3 to ~100 ms. The available experimental evidence suggests that the two transients are due to the presence of two conformations which do not interconvert within the time frame of the experiment. Computational studies revealed that the plasticity of protein structure is able to define a branched pathway connecting the ligand binding site and the solvent. This allowed to build a kinetic model capable of describing the complete time course of the CO rebinding kinetics to Tf-trHb. © 2012 Marcelli et al.
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