Descripción: Fil:Wisniacki, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.

Descripción: Aims. We study the modes and stability of non-isothermal coronal loop models with different intensity values of the equilibrium magnetic field. Methods. We use an energy principle obtained via non-equilibrium thermodynamic arguments. The principle is expressed in terms of Hermitian operators and allow to consider together the coupled system of equations: the balance of energy equation and the equation of motion. Results. We determine modes characterized as long - wavelength disturbances that are present in inhomogeneous media. This character of the system introduces additional difficulties for the stability analysis because the inhomogeneous nature of the medium determines the structure of the disturbance, which is no longer sinusoidal. Moreover, another complication is that we obtain a continuous spectrum of stable modes in addition to the discrete one. Conclusions. We obtain a unique unstable mode with a characteristic time that is comparable with the characteristic life-time observed for loops. The feasibility of wave-based and flow-based models is examined. © ESO 2006.

Descripción: Aims.We investigate the morphology at low radio frequencies of the supernova remnant (SNR) IC 443 in detail and accurately establish its radio continuum spectral properties. Methods. We used the VLA in multiple configurations to produce high-resolution radio images of IC 443 at 74 and 330 MHz. From these data we produced the first sensitive, spatially resolved spectral analysis of the radio emission at long wavelengths. The changes with position in the radio spectral index were correlated with data in near infrared (NIR) from 2MASS, in gamma-rays from VERITAS, and with the molecular 12CO (J = 1-0) line emission. Results. The new image at 74 MHz has HPBW = 35′′ and rms = 30 mJy beam-1 and at 330 MHz HPBW = 17′′and rms = 1.7 mJy beam-1. The integrated flux densities for the whole SNR are S SNR 74MHz = 470 ± 51 Jy and S SNR 330MHz = 248 ± 15 Jy. Improved estimates of the integrated spectrum were derived taking a turnover into account to fit the lowest frequency measurements in the literature. Combining our measurements with existing data, we derive an integrated spectral index α 10700MHz 10MHz =-0.39 ± 0.01 with a free-free continuum optical depth at 330 MHz 330 ∼ 7 × 10 -4 (τ10 = 1.07); all measurements above ∼10 MHz are equally consistent with a power law spectrum. For the pulsar wind nebula associated with the compact source CXOU J061705.3+222127, we calculated S PWN 330MHz = 0.23±0.05 Jy, SPWN 1420MHz = 0.20±0.04 Jy, and α8460MHz 330MHz ∼ 0.0. Substantial variations are observed in spectral index between 74 and 330 MHz across IC 443. The flattest spectral components (-0.25 = a =-0.05) coincide with the brightest parts of the SNR along the eastern border, with an impressive agreement with ionic lines as observed in the 2MASS J and H bands. The diffuse interior of IC 443 has a spectrum steeper than found anywhere in the SNR (-0.85 = a =-0.6), while the southern ridge again has a flatter spectrum (a ∼-0.4). With the available statistics the VERITAS .-ray emission strikingly matches the CO distribution, but no clear evidence is found for a morphological correlation between the TeV distribution and radio emission. Conclusions. The excellent correspondence between the eastern radio flattest spectrum region and NIR ionic lines strongly suggests that the passage of a fast, dissociating J-type shock across the interacting molecular cloud dissociated the molecules and ionized the gas.We therefore conclude that thermal absorption at 74 MHz (τ74 up to ∼0.3) is responsible for the localized spectral index flattening observed along the eastern border of IC 443. Towards the interior of IC 443, the spectrum is consistent with those expected from linear diffusive shock acceleration, while the flatter spectrum in the southern ridge is a consequence of the strong shock/molecular cloud interaction. © 2011 ESO.

Descripción: Rapidly rotating turbulent flow is characterized by the emergence of columnar structures that are representative of quasi-two-dimensional behavior of the flow. It is known that when energy is injected into the fluid at an intermediate scale L f, it cascades towards smaller as well as larger scales. In this paper we analyze the flow in the inverse cascade range at a small but fixed Rossby number, Ro f≈0.05. Several numerical simulations with helical and nonhelical forcing functions are considered in periodic boxes with unit aspect ratio. In order to resolve the inverse cascade range with reasonably large Reynolds number, the analysis is based on large eddy simulations which include the effect of helicity on eddy viscosity and eddy noise. Thus, we model the small scales and resolve explicitly the large scales. We show that the large-scale energy spectrum has at least two solutions: one that is consistent with Kolmogorov-Kraichnan-Batchelor-Leith phenomenology for the inverse cascade of energy in two-dimensional (2D) turbulence with a ∼k⊥-5/3 scaling, and the other that corresponds to a steeper ∼k⊥-3 spectrum in which the three-dimensional (3D) modes release a substantial fraction of their energy per unit time to the 2D modes. The spectrum that emerges depends on the anisotropy of the forcing function, the former solution prevailing for forcings in which more energy is injected into the 2D modes while the latter prevails for isotropic forcing. In the case of anisotropic forcing, whence the energy goes from the 2D to the 3D modes at low wave numbers, large-scale shear is created, resulting in a time scale τ sh, associated with shear, thereby producing a ∼k -1 spectrum for the total energy with the horizontal energy of the 2D modes still following a ∼k⊥-5/3 scaling. © 2012 American Physical Society.

Descripción: We put limits on the time variation of the electron mass in the early universe using observational primordial abundances of D, 4He, and 7Li, recent data from the cosmic microwave background, and the 2dFGRS power spectrum. Furthermore, we use these constraints together with other astronomical and geophysical bounds from the late universe to test the Barrow-Magueijo model for the variation in me. From our analysis we obtain -0.615 < Gω/c4 < -0.045 (3 σ interval), in disagreement with the result obtained in the original paper. © 2008. The American Astronomical Society. All rights reserved.

Descripción: In contrast to the wide spectrum of cytochrome P450 monooxygenases, there are only 2 heme-based dioxygenases in humans: tryptophan dioxygenase (hTDO) and indoleamine 2,3-dioxygenase (hIDO). hTDO and hIDO catalyze the same oxidative ring cleavage reaction of L-tryptophan to N-formyl kynurenine, the initial and rate-limiting step of the kynurenine pathway. Despite immense interest, the mechanism by which the 2 enzymes execute the dioxygenase reaction remains elusive. Here, we report experimental evidence for a key ferryl intermediate of hIDO that supports a mechanism in which the 2 atoms of dioxygen are inserted into the substrate via a consecutive 2-step reaction. This finding introduces a paradigm shift in our understanding of the heme-based dioxygenase chemistry, which was previously believed to proceed via simultaneous incorporation of both atoms of dioxygen into the substrate. The ferryl intermediate is not observable during the hTDO reaction, highlighting the structural differences between the 2 dioxygenases, as well as the importance of stereoelectronic factors in modulating the reactions.

Descripción: Correlation anisotropy emerges dynamically in magnetohydrodynamics (MHD), producing stronger gradients across the large-scale mean magnetic field than along it. This occurs both globally and locally, and has significant implications in space and astrophysical plasmas, including particle scattering and transport, and theories of turbulence. Properties of local correlation anisotropy are further documented here by showing through numerical experiments that the effect is intensified in more localized estimates of the mean field. The mathematical formulation of this property shows that local anisotropy mixes second-order with higher order correlations. Sensitivity of local statistical estimates to higher order correlations can be understood in connection with the stochastic coordinate system inherent in such formulations. We demonstrate this in specific cases, and illustrate the connection to higher order statistics by showing the sensitivity of local anisotropy to phase randomization, after which the global measure of anisotropy is recovered at all scales of averaging. This establishes that anisotropy of the local structure function is not a measure of anisotropy of the energy spectrum. Evidently, the local enhancement of correlation anisotropy is of substantial fundamental interest and must be understood in terms of higher order correlations, specifically fourth-order and above. © 2012. The American Astronomical Society. All rights reserved.

Descripción: The lipopeptidophosphoglycan from Trypanosoma cruzi is a glycosylated inositol‐phosphoceramide isolated from epimastigotes at the stationary phase of growth (4–5 days). We have now purified two similar glycoinositolphospholipids (glycoinositolphospholipid A and glycoinositolphospholipid B) from epimastigotes after the second day of culture growth. [3H]Palmitic acid was incorporated into 1‐O‐hexadecyl‐2‐O‐palmitoylglycerol in glycoinositolphospholipid A and into ceramide in glycoinositolphospholipid B. The lipids were released by incubation with glycosylphosphatidylinositol‐specific phospholipase C from Bacillus thuringiensis or by chemical methods. After alkaline hydrolysis, the lipids were analysed by GLC/MS. In glycoinositolphospholipid A the resulting lipids corresponded to 1‐O‐hexadecylglycerol and palmitic acid. The ceramide components in glycoinositolphospholipid B are sphinganine, palmitic acid and lignoceric acid. The oligosaccharides could be degraded by nitrous acid and further enzymic treatment showed that the two glycoinositolphospholipids isolated from T. cruzi share the common core structure of the glycosylphosphatidylinositol membrane anchors. The microheterogeneity was determined, as well as the substitution by galactose, and was mainly in the furanose configuration as was previously described for lipopeptidophosphoglycan. However, methylation analysis indicated that 20% of the galactose is in the pyranose from. Both glycoinositolphospholipids mainly differ in the lipid moiety. Copyright © 1993, Wiley Blackwell. All rights reserved

Descripción: Each day, advances in the instrumentation and operating protocols bring new applications and insights into the molecular processes of ultra violet-matrix assisted laser desorption/ionization-mass spectrometry (UV-MALDI MS), increasing its potential use. We report here an approach in which mass spectrometry analysis of sphingolipids has been performed using a fluorescent tag (nitrobenz-2-oxa-1, 3-diazole, NBD) covalently linked to the sphingoid base as matrix. Thus, different labeled-sphingolipids were analyzed: ceramide, dihydroceramide, acetylceramide, glucosylceramide, galactosylceramide, galactosyldihydroceramide. In addition an extract of glycosphingolipids obtained from epimastigote forms of Trypanosoma cruzi metabolically labeled with NBD-ceramide was analyzed. The goal of this work is to show that no matrix needs to be added for the mass spectrometry analysis as the same tag used to label the lipids may generate efficiently analyte ions to obtain high quality signals. © 2008 American Society for Mass Spectrometry.

Descripción: The Antarctic icefish Chaenocephalus aceratus lacks the globins common to most vertebrates, hemoglobin and myoglobin, but has retained neuroglobin in the brain. This conserved globin has been cloned, over-expressed and purified. To highlight similarities and differences, the structural features of the neuroglobin of this colourless-blooded fish were compared with those of the well characterised human neuroglobin as well as with the neuroglobin from the retina of the red blooded, hemoglobin and myoglobin-containing, closely related Antarctic notothenioid Dissostichus mawsoni. A detailed structural and functional analysis of the two Antarctic fish neuroglobins was carried out by UV-visible and Resonance Raman spectroscopies, molecular dynamics simulations and laser-flash photolysis. Similar to the human protein, Antarctic fish neuroglobins can reversibly bind oxygen and CO in the Fe 2+ form, and show six-coordination by distal His in the absence of exogenous ligands. A very large and structured internal cavity, with discrete docking sites, was identified in the modelled three-dimensional structures of the Antarctic neuroglobins. Estimate of the free-energy barriers from laser-flash photolysis and Implicit Ligand Sampling showed that the cavities are accessible from the solvent in both proteins. Comparison of structural and functional properties suggests that the two Antarctic fish neuroglobins most likely preserved and possibly improved the function recently proposed for human neuroglobin in ligand multichemistry. Despite subtle differences, the adaptation of Antarctic fish neuroglobins does not seem to parallel the dramatic adaptation of the oxygen carrying globins, hemoglobin and myoglobin, in the same organisms. © 2012 Giordano et al.

Descripción: We first study the seasonal and geographical behavior of gravity wave activity in the lower stratosphere over the southernmost Andes mountains and their prolongation in the Antarctic Peninsula by global positioning system (GPS) radio occultation (RO) temperature profiles, obtained between years 2002 and 2005 by the CHAllenging Minisatellite Payload (CHAMP) mission. The observed features complement observations in the same zone by other satellite passive remote sensing instruments, which are able to detect different height regions and other spectral intervals of the wave spectrum. Comparisons with previous GPS RO studies in smaller areas than the one covered in our analysis are also established. Significant seasonal variation of wave activity is observed in our work, in agreement with results from other instruments. The locations of significant cases indicate that topography is an important source. Some strong wave activity is also found over open ocean. Critical level filtering is shown to have an attenuation effect, implying that a large fraction of the observed activity can be considered to be an outcome of mountain waves. The studied region has a significant advantage as compared to other regions of our planet: it generates wavefronts nearly aligned with the North-South direction (almost parallel to the mountains), whereby this geometry favors the wave detection by the nearly meridional line of sight characterizing most of the GPS RO observations used. A distribution of the observed gravity waves in terms of amplitudes and wavelengths is also presented.

Descripción: Aims.We calculate the bounds on the variation in the fine structure constant at the time of primordial nucleosynthesis and at the time of neutral hydrogen formation. We used these bounds and other bounds from the late universe to test the Bekenstein model.Methods.We modified the Kawano code, CAMB, and CosmoMC to include the possible variation in the fine structure constant. We used observational primordial abundances of D, He, and Li, recent data from the cosmic microwave background, and the 2dFGRS power spectrum, to obtain bounds on the variation in . We calculated a piecewise solution to the scalar field equation of the Bekenstein model in two different regimes: i) matter and radiation, ii) matter and cosmological constant. We match both solutions with the appropriate boundary conditions. We performed a statistical analysis, using the bounds obtained from the early universe and other bounds from the late universe to constrain the free parameters of the model.Results.Results are consistent with no variation in for the early universe. Limits on are inconsistent with the scale length of the theory being larger than the Planck scale.Conclusions.In order to fit all observational and experimental data, the assumption > implied in Bekenstein's model has to be relaxed. © 2008 ESO.

Descripción: An experimental investigation was performed to study the effect on the free volume of the advance of the cross-linking reaction in a copolymer of styrene butadiene rubber by sulfur vulcanization. The dynamic modulus and loss tangent were evaluated over samples cured for different times at 433 K by dynamic mechanical tests over a range of frequencies between 5 and 80 Hz at temperatures between 200 and 300 K. Using the William-Landel-Ferry relationship, master curves were obtained at a reference temperature of 298 K and the coefficients [formula presented] and [formula presented] were evaluated. From these parameters the dependence of the free volume on the cure time is obtained. Positron annihilation lifetime spectroscopy was also used to estimate the size and number density of free volume sites in the material. The spectra were analyzed in terms of continuous distributions of free volume size. The results suggest an increase of the lower free volume size when cross linking takes place. Both techniques give similar results for the dependence of free volume on the time of cure of the polymer. © 2002 The American Physical Society.

Descripción: Aims. We study the stability and modes of non - isothermal coronal loop models with different intensity values of equilibrium twisted magnetic field.Methods. We use an energy principle obtained by means of non - equilibrium thermodynamic arguments. The principle is expressed in terms of Hermitian operators and enables us to consider the coupled system of equations, the balance of energy equation and the equation of motion, to obtain modes and eigenmodes in a spectrum ranging from short to long-wavelength disturbances without using weak varying approximations of the equilibrium parameters. Long-wavelength perturbations introduce additional difficulties because the inhomogeneous nature of the medium produce disturbances corresponding to continuous intervals of eigenfrequencies, which cannot be considered as purely sinusoidal.Results. We analyze the modification of periods, modes structure, and stability when the helicity, the magnetic field strength, and the radius of the fluxtube are varied. The efficiency of the damping due to the resonant absorption mechanism is analyzed in terms of modes that can either impulsively release or store magnetic energy.Conclusions. We find that the onset of the instability is associated with a critical value of the helicity and the magnetic energy content has a determinant role on the instability of the system with respect to the stabilizing effect of the resonant absorption mechanism. © 2008 ESO.

Descripción: Magnetohydrodynamic turbulence has been proposed as a mechanism for the heating of coronal active regions, and has therefore been actively investigated in recent years. According to this scenario, a turbulent regime is driven by footpoint motions. The energy being pumped this way into active region loops, is efficiently transferred to small scales due to a direct energy cascade. The ensuing generation of fine scale structures, which is a natural outcome of turbulent regimes, helps to enhance the dissipation of either waves or DC currents. We present an updated overview of recent results on turbulent coronal heating. To illustrate this theoretical scenario, we simulate the internal dynamics of a coronal loop within the reduced MHD approximation. The application of a stationary velocity field at the photospheric boundary leads to a turbulent stationary regime after several photospheric turnover times. This regime is characterized by a broadband power spectrum and energy dissipation rate levels compatible with the heating requirements of active region loops. Also, the energy dissipation rate displays a complex superposition of impulsive events, which we associate to the so-called nanoflares. A statistical analysis yields a power law distribution as a function of their energies, which is consistent with those obtained from observations. We also study the distributions of peak dissipation rate and duration of these events. © 2008 International Astronomical Union.

Descripción: Rhizobium leguminosarum is a soil bacterium that infects root hairs and induces the formation of nitrogen-fixing nodules on leguminous plants. Light, oxygen, and voltage (LOV)-domain proteins are bluelight receptors found in higher plants and many algae, fungi, and bacteria. The genome of R. leguminosarum bv. viciae 3841, a peanodulating endosymbiont, encodes a sensor histidine kinase containing a LOV domain at the N-terminal end (R-LOV-HK). R-LOV-HK has a typical LOV domain absorption spectrum with broad bands in the blue and UV-A regions and shows a truncated photocycle. Here we show that the R-LOV-HK protein regulates attachment to an abiotic surface and production of flagellar proteins and exopolysaccharide in response to light. Also, illumination of bacterial cultures before inoculation of pea roots increases the number of nodules per plant and the number of intranodular bacteroids. The effects of light on nodulation are dependent on a functional lov gene. The results presented in this work suggest that light, sensed by R-LOV-HK, is an important environmental factor that controls adaptive responses and the symbiotic efficiency of R. leguminosarum.