Descripción: In this work a small plasma jet is generated applying an ac high voltage (kV) of low frequency (50 Hz) between two disk-shaped electrodes with a hole in the center and separated by a centrally perforated dielectric material. A plasma jet emerges from the electrode system to the room air when a large air flow rate is passing through the holes, for inter-electrode voltage drops around 1 ÷ 3 kV. The electrical characteristics of the discharge, voltage and current were studied varying the applied voltage amplitude and the gas flow rate. It was found that the microplasma jet was stable during a long period of time and the gas temperature remained almost at room temperature. These characteristics make this discharge suitable for biological applications.

Descripción: In this work, an experimental study on the influence of the nozzle geometry on the physical properties of a cutting arc is reported. Ion current signals collected by an electrostatic probe sweeping across a 30 A oxygen cutting arc at 3.5 mm from the nozzle exit were registered for different nozzle lengths. The temperature and density radial profiles of the arc plasma were found in each case by an inversion procedure of these signals. A comparison between the obtained results shows that the shorter nozzle (RN = 0.50 mm, L N = 4.5 mm operated at 0.7 MPa and 35 Nl/min) produces a thinner and hotter arc than the larger nozzle (RN = 0.50 mm, LN = 9.0 mm operated at 1.1 MPa and 20 Nl/min). This behavior is attributed to the marked difference of gas flow rate due to the clogging effect. A smaller gas mass flow reduces the convective cooling at the arc border and decreases the power dissipation of the arc column, resulting in small axis temperatures. © 2009 IOP Publishing Ltd.

Descripción: A method is devised for estimating the two-time correlation function and the associated Eulerian decorrelation timescale in turbulence.With the assumptions of a single decorrelation time and a frozen-in flow approximation for the single-point analysis, the method compares two-point correlation measurements with single-point correlation measurements at the corresponding spatial lag. This method is applied to interplanetarymagnetic fieldmeasurements from the Advanced Composition Explorer and Wind spacecraft. An average Eulerian decorrelation time of 2.9 hr is found. This measures the total rate of distortion of turbulent fluid elements-including sweeping, nonlinear distortion, and wave propagation. © 2010. The American Astronomical Society. All rights reserved.

Descripción: Langmuir probe measurements in an atmospheric pressure direct current (dc) plasma jet are reported. Sweeping probes were used. The experiment was carried out using a dc non-transferred arc torch with a rod-type cathode and an anode of 5 mm diameter. The torch was operated at a nominal power level of 15 kW with a nitrogen flow rate of 25 Nl min -1. A flat ion saturation region was found in the current-voltage curve of the probe. The ion saturation current to a cylindrical probe in a high-pressure non local thermal equilibrium (LTE) plasma was modeled. Thermal effects and ionization/recombination processes inside the probe perturbed region were taken into account. Averaged radial profiles of the electron and heavy particle temperatures as well as the electron density were obtained. An electron temperature around 11 000 K, a heavy particle temperature around 9500 K and an electron density of about 4 × 10 22 m -3, were found at the jet centre at 3.5 mm downstream from the torch exit. Large deviations from kinetic equilibrium were found throughout the plasma jet. The electron and heavy particle temperature profiles showed good agreement with those reported in the literature by using spectroscopic techniques. It was also found that the temperature radial profile based on LTE was very close to that of the electrons. The calculations have shown that this method is particularly useful for studying spraying-type plasma jets characterized by electron temperatures in the range 9000-14 000 K. © 2012 American Institute of Physics.

Descripción: Background: Resolving the kinetics of agonist binding events separately from the subsequent channel gating processes requires the ability of applying and removing the agonist before channel gating occurs. No reported system has yet achieved pulses shorter than 100 μs, necessary to study nicotinic ACh receptor or AMPA receptor activation. Methodology/Principal Findings: Solution exchange systems deliver short agonist pulses by moving a sharp interface between a control and an experimental solution across a channel preparation. We achieved shorter pulses by means of an exchange system that combines a faster flow velocity, narrower partition between the two streams, and increased velocity and bandwidth of the movement of the interface. The measured response of the entire system was fed back to optimize the voltage signal applied to the piezoelectric actuator overcoming the spurious oscillations arising from the mechanical resonances when a high bandwidth driving function was applied. Optimization was accomplished by analyzing the transfer function of the solution exchange system. When driven by optimized command pulses the enhanced system provided pulses lasting 26 ± 1 μs and exchanging 93 ± 1% of the solution, as measured in the open tip of a patch pipette. Conclusions/Significance: Pulses of this duration open the experimental study of the molecular events that occur between the agonist binding and the opening of the channel. © 2012 Auzmendi et al.

Descripción: The dynamic behavior of the anode-arc-root at the nozzle surface of a plasma torch was experimentally investigated in this work. A gas (N2) vortex-stabilized non-transferred arc torch with a thoriated tungsten rod (2wt %) cathode (3.2 mm diameter) and a coaxial anode (5 mm diameter, 30 mm length) was used in the experiment. By using a sweeping Langmuir probe in floating condition, the voltage of the plasma jet outside the nozzle was inferred. Arc voltage waveforms were also obtained. Data have been obtained for an arc current of 100 A and a gas flow rate of 30 Nl min-1. A typical sawtooth shape (i.e., restrike mode) (with a fluctuating level of ≈ ± 25 %) and a dominant frequency of ≈ 6.5 kHz was observed in the arc voltage waveforms, which is attributed to anode-arc-root movements along the anode surface followed by a restrike at a certain point close to the cathode. By performing a time correlation between the probe and arc voltage oscillograms together with simple estimations, the amplitude of the movement of the arc-root along the anode surface as well its velocity were inferred.

Descripción: We determine how the differences in the treatment of the subfilter-scale physics affect the properties of the flow for three closely related regularizations of Navier-Stokes. The consequences on the applicability of the regularizations as subgrid-scale (SGS) models are also shown by examining their effects on superfilter-scale properties. Numerical solutions of the Clark-α model are compared to two previously employed regularizations, the Lagrangian-averaged Navier-Stokes α-model (LANS-α) and Leray-α, albeit at significantly higher Reynolds number than previous studies, namely, Re≈3300, Taylor Reynolds number of Re≈790, and to a direct numerical simulation (DNS) of the Navier-Stokes equations. We derive the de Kármán-Howarth equation for both the Clark-α and Leray-α models. We confirm one of two possible scalings resulting from this equation for Clark-α as well as its associated k-1 energy spectrum. At subfilter scales, Clark-α possesses similar total dissipation and characteristic time to reach a statistical turbulent steady state as Navier-Stokes, but exhibits greater intermittency. As a SGS model, Clark-α reproduces the large-scale energy spectrum and intermittency properties of the DNS. For the Leray-α model, increasing the filter width α decreases the nonlinearity and, hence, the effective Reynolds number is substantially decreased. Therefore, even for the smallest value of α studied Leray-α was inadequate as a SGS model. The LANS-α energy spectrum ∼k1, consistent with its so-called "rigid bodies," precludes a reproduction of the large-scale energy spectrum of the DNS at high Re while achieving a large reduction in numerical resolution. We find, however, that this same feature reduces its intermittency compared to Clark-α (which shares a similar de Kármán-Howarth equation). Clark-α is found to be the best approximation for reproducing the total dissipation rate and the energy spectrum at scales larger than α, whereas high-order intermittency properties for larger values of α are best reproduced by LANS-α. © 2008 American Institute of Physics.

Descripción: Context. A magnetic cloud (MC) is a magnetic flux rope in the solar wind (SW), which, at 1 AU, is observed ∼2-5 days after its expulsion from the Sun. The associated solar eruption is observed as a coronal mass ejection (CME).Aims. Both the in situ observations of plasma velocity distribution and the increase in their size with solar distance demonstrate that MCs are strongly expanding structures. The aim of this work is to find the main causes of this expansion and to derive a model to explain the plasma velocity profiles typically observed inside MCs.Methods. We model the flux rope evolution as a series of force-free field states with two extreme limits: (a) ideal magneto-hydrodynamics (MHD) and (b) minimization of the magnetic energy with conserved magnetic helicity. We consider cylindrical flux ropes to reduce the problem to the integration of ordinary differential equations. This allows us to explore a wide variety of magnetic fields at a broad range of distances to the Sun.Results. We demonstrate that the rapid decrease in the total SW pressure with solar distance is the main driver of the flux-rope radial expansion. Other effects, such as the internal over-pressure, the radial distribution, and the amount of twist within the flux rope have a much weaker influence on the expansion. We demonstrate that any force-free flux rope will have a self-similar expansion if its total boundary pressure evolves as the inverse of its length to the fourth power. With the total pressure gradient observed in the SW, the radial expansion of flux ropes is close to self-similar with a nearly linear radial velocity profile across the flux rope, as observed. Moreover, we show that the expansion rate is proportional to the radius and to the global velocity away from the Sun.Conclusions. The simple and universal law found for the radial expansion of flux ropes in the SW predicts the typical size, magnetic structure, and radial velocity of MCs at various solar distances. © 2009 ESO.

Descripción: Context: Observations of magnetic clouds (MCs) are consistent with the presence of flux ropes detected in the solar wind (SW) a few days after their expulsion from the Sun as coronal mass ejections (CMEs). Aims: Both the in situ observations of plasma velocity profiles and the increase of their size with solar distance show that MCs are typically expanding structures. The aim of this work is to derive the expansion properties of MCs in the inner heliosphere from 0.3 to 1 AU. Methods: We analyze MCs observed by the two Helios spacecraft using in situ magnetic field and velocity measurements. We split the sample in two subsets: those MCs with a velocity profile that is significantly perturbed from the expected linear profile and those that are not. From the slope of the in situ measured bulk velocity along the Sun-Earth direction, we compute an expansion speed with respect to the cloud center for each of the analyzed MCs. Results: We analyze how the expansion speed depends on the MC size, the translation velocity, and the heliocentric distance, finding that allMCs in the subset of non-perturbed MCs expand with almost the same non-dimensional expansion rate (ζ).We find departures from this general rule for ζ only for perturbed MCs, and we interpret the departures as the consequence of a local and strong SW perturbation by SW fast streams, affecting the MC even inside its interior, in addition to the direct interaction region between the SW and the MC. We also compute the dependence of the mean total SW pressure on the solar distance and we confirm that the decrease of the total SW pressure with distance is the main origin of the observed MC expansion rate. We found that ζ was 0.91 ± 0.23 for non-perturbed MCs while ζ was 0.48 ± 0.79 for perturbed MCs, the larger spread in the last ones being due to the influence of the solar wind local environment conditions on the expansion. © ESO 2010.

Descripción: The spontaneous non-obese diabetic (NOD) mouse model of Sjögren's syndrome provides a valuable tool to study the onset and progression of both the autoimmune response and secretory dysfunction. Our purpose was to analyse the temporal decline of salivary secretion in NOD mice in relation to the autoimmune response and alterations in various signalling pathways involved in saliva secretion within each salivary gland. A progressive loss of nitric oxide synthase activity in submandibular and parotid glands started at 12 weeks of age and paralleled the decline in salivary secretion. This defect was associated with a lower response to vasoactive intestinal peptide in salivary flow rate, cAMP and nitric oxide/cGMP production. No signs of mononuclear infiltrates or local cytokine production were detectable in salivary glands in the time period studied (10-16 weeks of age). Our data support a disease model for sialadenitis in NOD mice in which the early stages are characterized by defective neurotransmitter-mediated signalling in major salivary glands that precedes the autoimmune response. © 2005 British Society for Immunology.

Descripción: The effect of rotation is considered to become important when the Rossby number is sufficiently small, as is the case in many geophysical and astrophysical flows. Here we present direct numerical simulations to study the effect of rotation in flows with moderate Rossby numbers (down to Ro ≈ 0.03) but at Reynolds numbers large enough to observe the beginning of a turbulent scaling at scales smaller than the energy injection scale. We use coherent forcing at intermediate scales, leaving enough room in the spectral space for an inverse cascade of energy to also develop. We analyze the spectral behavior of the simulations, the shell-to-shell energy transfer, scaling laws and intermittency, as well as the geometry and the anisotropy of the structures in the flow. At late times, the direct transfer of energy at small scales is mediated by interactions with the largest scale in the system, the energy containing eddies with k⊥ ≈ 1, where ⊥ refers to wavevectors perpendicular the axis of rotation. The transfer between modes with wavevector parallel to the rotation is strongly quenched. The inverse cascade of energy at scales larger than the energy injection scale is nonlocal, and energy is transferred directly from small scales to the largest available scale. We observe both a direct and inverse cascade of energy at high rotation rate, indicative that these cascades can take place simultaneously. Also, as time evolves and the energy piles up at the large scales, the intermittency of the direct cascade of energy is preserved while corrections due to intermittency are found to be the same (within error bars) as in homogeneous nonrotating turbulence. © 2009 American Institute of Physics.

Descripción: We investigate the ideal and incompressible magnetohydrodynamic (MHD) equations in three space dimensions for the development of potentially singular structures. The methodology consists in implementing the fourfold symmetries of the Taylor-Green vortex generalized to MHD, leading to substantial computer time and memory savings at a given resolution; we also use a regridding method that allows for lower-resolution runs at early times, with no loss of spectral accuracy. One magnetic configuration is examined at an equivalent resolution of 61443 points and three different configurations on grids of 40963 points. At the highest resolution, two different current and vorticity sheet systems are found to collide, producing two successive accelerations in the development of small scales. At the latest time, a convergence of magnetic field lines to the location of maximum current is probably leading locally to a strong bending and directional variability of such lines. A novel analytical method, based on sharp analysis inequalities, is used to assess the validity of the finite-time singularity scenario. This method allows one to rule out spurious singularities by evaluating the rate at which the logarithmic decrement of the analyticity-strip method goes to zero. The result is that the finite-time singularity scenario cannot be ruled out, and the singularity time could be somewhere between t=2.33 and t=2.70. More robust conclusions will require higher resolution runs and grid-point interpolation measurements of maximum current and vorticity. © 2013 American Physical Society.

Descripción: In the region of the Main Andes of San Juan, exists a high concentration of landslide deposits that can originate natural dams. The outburst of these dams generated by slide is a common process in this morphology. The analyses of satellite imagery and air photos of previous years allow reconstructing the characteristics and the origin of the slides that formed those natural dams. In this region of the Main Andes, the rapid uplift and erosion of the mountain chain has created abrupt slopes and an internal structure in the materials that determined an increase of the susceptibility to the slope collapse without triggers as the seismic activity or precipitations. The objectives of the present work are (1) to analyze the causes that formed the natural dam and produced the landslide dam and development of the Los Erizos lagoon in the middle course of the Santa Cruz river; (2) the outburst of the dam and their consequent flood; and (3) the morphologic characteristic of the study region. The temporary comparison between air photos with satellite imageries indicates that the natural dam was generated by slip of the western hillside of the Santa Cruz mountain range, located below the Cerro Estrella. However, the constant water flow to the lagoon, has increased its water level until reaching the maximum benchmark of the dam, produced the beginning of the lake drainage and weakening of the dam causing the outburst and its consequent flood. Based on satellite imagery, a temporary analysis of the lagoon is presented, indicating that monitoring is an important tool to alert in the whole San Juan river basin for possible natural dikes, preventing hazards to the populations down water. The alluvium of November 12 of 2005, discharged 32.100.000 m3 in 67 minutes and traveled 254 km in less than 12 hours.

Descripción: Two populations of human natural killer (NK) cells can be identified in peripheral blood. The majority are CD3-CD56dim cells while the minority exhibits a CD3-CD56bright phenotype. In vitro evidence indicates that CD56bright cells are precursors of CD56dim cells, but in vivo evidence is lacking. Here, we studied NK cells from a patient that suffered from a melanoma and opportunistic fungal infection during childhood. The patient exhibited a stable phenotype characterized by a reduction in the frequency of peripheral blood CD3-CD56dim NK cells, accompanied by an overt increase in the frequency and absolute number of CD3-CD56bright cells. These NK cells exhibited similar expression of perforin, CD57 and CD158, the major activating receptors CD16, NKp46, NKG2D, DNAM-1, and 2B4, as well as the inhibitory receptor CD94/NKG2A, on both CD56bright and CD56dim NK cells as healthy controls. Also, both NK cell subpopulations produced IFN-γ upon stimulation with cytokines, and CD3-CD56dim NK cells degranulated in response to cytokines or K562 cells. However, upon stimulation with cytokines, a substantial fraction of CD56dim cells failed to up-regulate CD57 and CD158, showed a reduction in the percentage of CD16+ cells, and CD56bright cells did not down-regulate CD62L, suggesting that CD56dim cells could not acquire a terminally differentiated phenotype and that CD56bright cells exhibit a maturation defect that might result in a potential altered migration pattern. These observations, support the notion that NK cells of this patient display a maturation/activation defect that precludes the generation of mature NK cells at a normal rate accompanied by CD56dim NK cells that cannot completely acquire a terminally differentiated phenotype. Thus, our results provide evidence that support the concept that in vivo CD56bright NK cells differentiate into CD56dim NK cells, and contribute to further understand human NK cell ontogeny. © 2012 Domaica et al.