Descripción: We investigate an unsteady plane viscous gravity current of silicone oil on a horizontal glass substrate. Within the lubrication approximation with gravity as the dominant force, this current is described by the nonlinear diffusion equation [Formula Presented]=([Formula Presented][Formula Presented][Formula Presented] (φ is proportional to the liquid thickness h and m=3>0), which is of interest in many other physical processes. The solutions of this equation display a fine example of the competition between diffusive smoothening and nonlinear steepening. This work concerns the so-called waiting-time solutions, whose distinctive character is the presence of an interface or front, separating regions with h≠/0 and h=0, that remains motionless for a finite time interval [Formula Presented] meanwhile a redistribution of h takes place behind the interface. We start the experiments from an initial wedge-shape configuration [h(x)≊[Formula Presented]([Formula Presented]-x)] with a small angle ([Formula Presented]⩽0.12 rad). In this situation, the tip of the wedge, situated at [Formula Presented] from the rear wall (15 cm⩽[Formula Presented]⩽75 cm), waits at least several seconds before moving. During this waiting stage, a region characterized by a strong variation of the free surface slope (corner layer) develops and propagates toward the front while it gradually narrows and [Formula Presented]h/∂[Formula Presented] peaks. The stage ends when the corner layer overtakes the front. At this point, the liquid begins to spread over the uncovered substrate. We measure the slope of the free surface in a range ≊10 cm around [Formula Presented], and, by integration, we determine the fluid thickness h(x) there. We find that the flow tends to a self-similar behavior when the corner layer position tends to [Formula Presented]; however, near the end of the waiting stage, it is perturbed by capillarity. Even if some significant effects are not included in the above equation, the main properties of its solutions are well displayed in the experiments © 1996 The American Physical Society.

Descripción: A Singular Value Decomposition (SVD) analysis was performed jointly on extreme temperature events in Argentina and sea surface temperature (SST) in the South Atlantic and South Pacific. Sea level pressure (SLP) patterns associated with the first SVD coupled mode were also analyzed. Winter is the season of the year that is best represented by the first mode, accounting for up to 70% of the winter covariance between temperature events and SST. The warm and cold events in Argentina are essentially a consequence of the creation of meridional atmospheric circulations over the continent. Such atmospheric patterns result from displacements and intensity changes of the subtropical anticyclones over the oceans and of the continental low-pressure center in northwestern Argentina. The temperature events in southern Argentina are also closely related to the warming and cooling of the coastal waters in the South Atlantic and South Pacific. The analysis suggests that in summer and winter, high (low) occurrence of warm events and low (high) occurrence of cold events are related to similar oceanic and atmospheric circulation situations. The temperature events in Argentina show higher correlation with the Atlantic than with the Pacific, which reflects the importance of the "orographic barrier" of the Andes Mountains in driving the atmospheric circulation. The only exception to this rule concerns the warm events in spring, for which the warming of the equatorial Pacific (the ENSO pattern) appears as the dominant mode. The temporal patterns of the temperature events in Argentina exhibit significant interannual variability in fall, winter, and spring, with periods of 3 to 5 years. The summer patterns suggest a very low-frequency variation with a period longer than 20 years. Copyright 2003 by the American Geophysical Union.

Descripción: Molecular dynamics simulations of water in cylindrical hydrophilic pores with diameters of 1.5 and 3 nm were performed to explore the phase behavior and the nucleation dynamics of the confined fluid as a function of the percentage of volume filled f. The interactions of water with the pore wall were considered to be identical to the interactions between water molecules. At low water contents, all the water is adsorbed to the surface of the pore. A second phase consisting of a liquid plug appears at the onset filling for capillary condensation, fonset =27% and 34% for the narrow and wide pores, respectively. In agreement with experimental results for silica pores, the liquid phase appears close to the equilibrium filling feq in the 1.5 nm pore and under conditions of strong surface supersaturations for the 3 nm pore. After condensation, two phases, a liquid plug and a surface-adsorbed phase, coexist in equilibrium. Under conditions of phase coexistence, the water surface density Tcoex was found to be independent of the water content and the diameter of the pore. The value of Tcoex found in the simulations (∼3 nm-2) is in good agreement with experimental results for silica pores, suggesting that the interactions of water with silica and with itself are comparable. The surface-adsorbed phase at coexistence is a sparse monolayer with a structure dominated by small water clusters. We characterize the density and structure of the liquid and surface phases, the nucleation mechanism of the water plug, and the effect of surface hydrophilicity on the two-phase equilibrium and hysteresis. The results are discussed in light of experiments and previous simulations. © 2010 American Institute of Physics.

Descripción: The purpose of this review is to examine and discuss the ways in which water is organized at the interface of a biological membrane. The relevance of this structure to the surface properties and to the adsorption of proteins in membranes is also analized. The approach is based on the idea that cell functions are confined to a restricted water media, the cell interior, in which the proximity of the membrane may be key to regulating the enzyme activity and the cell membrane permeability. As the lipid bilayer is the structural base of cell membranes, the distribution of water in the surface sites of a phospholipid membrane is analyzed by means of Fourier Transform spectrometry. The polarization of water at the surface was looked into through the measure of surface potentials and the dynamics of the surface hydration by cyclic voltammetry. Modification of these properties by the replacement of water by polyol molecules such as trehalose and phloretin and by the insertion of aqueous soluble enzymes, has also been investigated.

Descripción: Bamboos are prominent components of many tropical ecosystems, yet little is known about the physiological mechanisms utilized by these gigantic forest grasses. Here, we present data on the water transport properties of Chusquea ramosissima and Merostachys claussenii, monocarpic bamboo grasses native to the subtropical Atlantic forests of Argentina. C. ramosissima and M. claussenii differed in their growth form and exhibited contrasting strategies of water transport. Maximum xylem hydraulic conductivity of C. ramosissima culms was 2-fold higher than that of M. claussenii. C. ramosissima cavitated at relatively high water potentials (50% loss of conductivity at ≥1 MPa), whereas M. claussenii was more drought tolerant (50% loss at ≤3 MPa). Both species exhibited significant loss of hydraulic conductivity during the day, which was reversed overnight due to the generation of root pressure. The photosynthetic capacities of both bamboo species, estimated based on electron transport rates, were moderate, reflecting both the large amount of leaf area supported by culms and diurnal loss of hydraulic conductivity due to cavitation. Leaf hydraulic conductance was also relatively low for both species, congruent with their modest photosynthetic capacities. Within its native range, C. ramosissima is highly invasive due to its ability to colonize and persist in both forest gaps and land cleared for agriculture. We propose that a highly vulnerable vasculature, coupled with diurnal root pressure and an allometry that allows substantial leaf area to be supported on relatively slender culms, are key traits contributing to the ecological success of C. ramosissima. © 2009 American Society of Plant Biologists.

Descripción: In the slugs and snails odor input signal, partly processed by the tentacle ganglion, propagates through the tentacle nerve (TN) to the cerebral ganglion, initially activating the meso-meta-region and finally the procerebral region (PC). The PC, equivalent to mammalian olfactory bulb, exerts slow spontaneous neuroelectrical oscillation, which changes its frequency and amplitude pattern responding to stimulus input. This has been related to a mechanism of signal processing for odor encoding. Three neuronal substructures, the cell mass (CM), the terminal mass (TM) and internal mass (IM) form the PC. Records from IM and CM have extensively been studied, but those from TM have scarcely been investigated. In the present study we aimed to clarify network dynamics among these cell ensembles with particular interest in the property of TM. Methods: We isolated the cerebral ganglia from the slug Incilaria together with TNs. We applied to TN electrical stimulation of weak to strong intensities (0.1 - 1.0 μA) and recorded activities at the three loci of PC by glass suction electrodes at a sampling rate of 200 Hz. The data were stored on hard drive and later off-line analysed by wavelet tools. Results: Wavelet analysis revealed that the major power of the spontaneous oscillations laid below 1.6 Hz. Namely, in the Incilaria PC, mainly the frequency components < 1.6 Hz take part in the dynamical signal processing. The frequency components, that are time-dependently, interacting with each other, contribute together to altering total entropy of a cell mass at a given time. Notably, the 0.1 - 0.2 Hz component contributing most strongly to total energy attributes most to dropping entropy ("ordering of neuronal state"). Response to the weakest stimulus is most sensitively elicited as "desynchronization" in TM-IM, but that to the stronger stimuli, as "synchronization or frequency ordering" in TM-CM, and finally "synchronization" in TM-IM-CM (the whole PC). The fact that the entropy of TM in general remains lower than IM and CM regardless with stimulation suggests that the neurons of TM are in more ordered state than the other masses playing some governing function in the procerebral network. © 2007 American Institute of Physics.

Descripción: We examine the decomposition of forced Taylor-Green and Arn'old-Beltrami-Childress (ABC) flows into coherent and incoherent components using an orthonormal wavelet decomposition. We ask whether wavelet coefficient thresholding based on the Donoho-Johnstone criterion can extract a coherent vortex signal while leaving behind Gaussian random noise. We find that no threshold yields a strictly Gaussian incoherent component, and that the most Gaussian incoherent flow is found for data compression lower than that achieved with the fully iterated Donoho-Johnstone threshold. Moreover, even at such low compression, the incoherent component shows clear signs of large-scale spatial correlations that are signatures of the forcings used to drive the flows. © 2012 American Institute of Physics.

Descripción: Fil:Mininni, P.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.

Descripción: We define a partial order on the set of faces of the permutohedron, which extends the weak Bruhat order of the symmetric group. The restriction of this order gives a partially ordered set structure to the set of faces of the associahedron, which extends the Tamari order defined on the set of planar binary rooted trees. These orders are used to describe associative algebra structures on the vector spaces spanned by the set of faces of the permutohedra on one hand, and on the vector space spanned by the set of planar rooted trees on the other hand. Our results extend the description of the dendriform algebra structure on the spaces spanned by permutations and by planar binary trees in terms of the weak Bruhat order and the Tamari order, respectively, obtained by J.-L. Loday and the second author in [J.-L. Loday, M. Ronco, Order structure and the algebra of permutations and of planar binary trees, J. Algebraic Combin. 15 (2002) 253-270]. © 2005 Elsevier Inc. All rights reserved.

Descripción: We study the existence of weak solutions to the equation Δpu = |u|p-2u + f(x, u) with the nonlinear boundary condition |∇u|p-2∂u/∂v = λ|u|p-2u - h(x, u). We assume Landesman-Lazer type conditions and use variational arguments to prove the existence of solutions.

Descripción: In this work we take under consideration the Cauchy problem for the Schrödinger-Poisson type equation i t u=- x2 u+V (u) u-f (∫u∫2) u, where f represents a local nonlinear interaction (we take into account both attractive and repulsive models) and V is taken as a suitable solution of the Poisson equation V=12 ∫x∫ (C- ∫u∫2), C Cc∞ is the doping profile or impurities. We show that this problem is locally well posed in the weighted Sobolev spaces Hs { Hs (R): (1+ x2) 12 ∫∫2 <∞} with s1, which means the local existence, uniqueness, and continuity of the solution with respect to the initial data. Moreover, under suitable assumptions on the local interaction, we show the existence of global solutions. Finally, we establish that for s1 local in time and space, smoothing effects are present in the solution; more precisely, in this problem there is locally a gain of half a derivative. © 2007 American Institute of Physics.

Descripción: Background: The vast computational resources that became available during the past decade enabled the development and simulation of increasingly complex mathematical models of cancer growth. These models typically involve many free parameters whose determination is a substantial obstacle to model development. Direct measurement of biochemical parameters in vivo is often difficult and sometimes impracticable, while fitting them under data-poor onditions may result in biologically implausible values. Results: We discuss different methodological approaches to estimate parameters in complex biological models. We make use of the high computational power of the Blue Gene technology to perform an extensive study of the parameter space in a model of avascular tumor growth. We explicitly show that the landscape of the cost function used to optimize the model to the data has a very rugged surface in parameter space. This cost function has many local minima with unrealistic solutions, including the global minimum corresponding to the best fit. Conclusions: The case studied in this paper shows one example in which model parameters that optimally fit the data are not necessarily the best ones from a biological point of view. To avoid force-fitting a model to a dataset, we propose that the best model parameters should be found by choosing, among suboptimal parameters, those that match criteria other than the ones used to fit the model. We also conclude that the model, data and optimization approach form a new complex system and point to the need of a theory that addresses this problem more generally. © 2010 Fernandez Slezak et al.

Descripción: A palaeomagnetic study was performed on three Neoproterozoic sedimentary successions exposed in central and eastern Uruguay in order to better constrain the palaeogeographic evolution of the Río de la Plata craton by the latest Proterozoic. These units comprise the latest Ediacaran to Early Cambrian calcareous Cerro Victoria Fm., exposed in the central Nico Pérez terrane, the late Ediacaran clastic Yerbal Fm. and the calcareous Polanco Fm., exposed in the Dom Feliciano belt, and the Ediacaran clastic Rocha Fm. exposed in the easternmost Punta del Este suspect terrane. The study showed that none of the units carry the original remanence and that they were affected by remagnetizations. The most widespread remagnetization is shown by the Cerro Victoria, Yerbal and Rocha Formations. A dual-polarity post-folding remanence, carried by hematite, was isolated in all these units. Mean directions from them are CV (Cerro Victoria): Dec: 179.1°, Inc: 59.0°, α95: 2.9°, n = 79 samples; Y (Yerbal): Dec: 182.4°, Inc: 61.9°, α 95: 4.5°, n = 38 samples; R (Rocha): Dec: 4.2°, Inc: -64.9°, α 95: 2.7°, n = 42 samples. Their corresponding pole positions are virtually identical (CV: 82.6°S, 309.3°E, A95: 3.9° Y: 77.0°S, 298.4°E, A95: 5.9° R: 76.6°S, 291.0°E, A95: 4.2°) suggesting the same magnetization age and process. Comparison with the South American path suggests the Late Permian-Early Triassic or the latest Cretaceous-Palaeogene as the most likely times for its acquisition. These poles are also identical to the pole of the Late Proterozoic La Tinta Fm., which is interpreted as affected by the same remagnetization, and therefore, invalid for palaeogeographic reconstructions. A different magnetic component, also dual polarity and post-folding, was defined at two sites of the Cerro Victoria Fm. and other two of the Polanco Fm., possibly carried by magnetite. The mean geomagnetic poles from both units (CVc: 4.2°N, 343.2°E, A95: 13.8° and P: 3.2°N, 325.8°E, A95: 15.2°) fall on the Middle Cambrian to Early Ordovician segment of the Gondwana APWP, suggesting that age for the remagnetization. This permits to establish the age of the tectonic event affecting the Neoproterozoic units of the Río de la Plata craton as Early Cambrian (ca. 525 Ma.). The available palaeomagnetic poles for this craton permit to constrain its palaeogeographic evolution only since approximately 600 Ma. © 2008 The Authors Journal compilation © 2008 RAS.

Descripción: As in most insect groups, host plant shifts in cactophilic Drosophila represent environmental challenges as flies must adjust their developmental programme to the presence of different chemical compounds and/or to a microflora that may differ in the diversity and abundance of yeasts and bacteria. In this context, wing morphology provides an excellent opportunity to investigate the factors that may induce changes during development. In this work, we investigated phenotypic plasticity and developmental instability of wing morphology in flies on the cactophilic Drosophila buzzatii and Drosophila koepferae raised on alternative breeding substrates. We detected significant differences in wing size between and within species, and between flies reared on different cactus hosts. However, differences in wing shape between flies emerged from different cactus hosts were not significant either in D. buzzatii or in D. koepferae. Our results also showed that morphological responses involved the entire organ, as variation in size and shape correlated between different portions of the wing. Finally, we studied the effect of the rearing cactus host on developmental instability as measured by the degree of fluctuating asymmetry (FA). Levels of FA in wing size were significantly greater in flies of both species reared in non-preferred when compared with those reared in preferred host cacti. Our results are discussed in the framework of an integrative view aimed at investigating the relevance of host plant shifts in the evolution of the guild of cactophilic Drosophila species that diversified in South America. © 2007 The Authors.

Descripción: A central issue in evolutionary biology is to understand the mechanisms promoting morphological evolution during speciation. In a previous study, we showed that the Neotropical cactophilic sibling species Drosophila gouveai and Drosophila antonietae can be reared in media prepared with their presumptive natural host plants (Pilosocereus machrisis and Cereus hildmaniannus) and that egg to adult viability is not independent of the cactus host. In the present study, we investigate the effects of ecological and genetic factors on interspecific divergence in wing morphology, in relation to the pattern of wing venation and phenotypic plasticity in D. gouveai and D. antonietae, by means of the comparative analysis of isofemale lines reared in the two cactus hosts. The species differed significantly in wing size and shape, although specific differences were mainly localized in a particular portion of the wing. We detected significant variation in form among lines, which was not independent of the breeding cactus, suggesting the presence of genetic variation for phenotypic plasticity and wing shape variation in both species. We discuss the results considering the plausible role of host plant use in the evolutionary history of cactophilic Drosophila inhabiting the arid zones of South America. © 2008 The Linnean Society of London.

Descripción: We conducted a whole year research on the ecology of Mansonia indubitans and Ma. titillans in Macáes Pond, Costanera Sur Reserve, Buenos Aires, Argentina. The usage of different floating plants by immature instars and their overwintering was analized. The percentage of usage of the available floating macrophytes (Pistia, Limnobium, and Salvinia) by the larvae and pupae was studied. Also, we defined positivity (P+) as the percentage of plants with immature instars for each plant genus on a monthly basis. Ma. immature instars were captured throughout the year and Pistia was the resource most commonly exploited by the mosquitoes. The percentage of fourth-instar larvae and pupae on Pistia roots with respect to total immature instars captured was assessed on a monthly and seasonal basis. The proportion of fourth-instar larvae and pupae from both species of Mansonia on water lettuce roots, showed significant differences between months and seasons. Our results suggest that the populations of Ma. indubitans and Ma. titillans in Macáes Pond, survive during winter mainly as fourth-instar larvae.

Descripción: We study the role of fundamental constants in an updated recombination scenario. We focus on the time variation of the fine structure constant α, and the electron mass me in the early Universe, and put bounds on these quantities by using data from CMB including WMAP 5-yr release and the 2dFGRS power spectrum. We analyze how the constraints are modified when changing the recombination scenario. © 2008 Elsevier B.V. All rights reserved.

Descripción: Xanthan is the major exopolysaccharide secreted by Xanthomonas spp. Despite its diverse roles in bacterial pathogenesis of plants, little is known about the real implication of this molecule in Xanthomonas pathogenesis. In this study we show that in contrast to Xanthomonas campestris pv campestris strain 8004 (wild type), the xanthan minus mutant (strain 8397) and the mutant strain 8396, which is producing truncated xanthan, fail to cause disease in both Nicotiana benthamiana and Arabidopsis (Arabidopsis thaliana) plants. In contrast to wild type, 8397 and 8396 strains induce callose deposition in N. benthamiana and Arabidopsis plants. Interestingly, treatment with xanthan but not truncated xanthan, suppresses the accumulation of callose and enhances the susceptibility of both N. benthamiana and Arabidopsis plants to 8397 and 8396 mutant strains. Finally, in concordance, we also show that treatment with an inhibitor of callose deposition previous to infection induces susceptibility to 8397 and 8396 strains. Thus, xanthan suppression effect on callose deposition seems to be important for Xanthomonas infectivity. © 2006 American Society of Plant Biologists.

Descripción: Xanthan is an industrially important exopolysaccharide produced by the phytopathogenic, gram-negative bacterium Xanthomonas campestris pv. campestris. It is composed of polymerized pentasaccharide repeating malts which are assembled by the sequential addition of glucose-1-phosphate, glucose, mannose, glucuronic acid, and mannose on a polyprenol phosphate carrier (L. Ielpi, R. O. Couso, and M. A. Dankert, J. Bacteriol. 175:2490- 2500, 1993). A cluster of 12 genes in a region designated xpsI or gum has been suggested to encode proteins involved in the synthesis and polymerization of the lipid intermediate. However, no experimental evidence supporting this suggestion has been published. In this work, from the biochemical analysis of a defined set of X. campestris gum mutants, we report experimental data for assigning functions to the products of the gum genes. We also show that the first step in the assembly of the lipid-linked intermediate is severely affected by the combination of certain gum and non- gum mutations. In addition, we provide evidence that the C-terminal domain of the gumD gene product is sufficient for its glucosyl-1-phosphate transferase activity. Finally, we found that alterations in the later stages of xanthan biosynthesis reduce the aggressiveness of X. campestris against the plant.

Descripción: The epsins are a family of adaptors involved in recruiting other endocytic proteins, binding of ubiquitylated cargo and induction of membrane curvature. These molecules bear a characteristic epsin N-terminal homology (ENTH) domain and multiple peptide motifs that mediate protein-protein interactions. We have previously demonstrated that the ENTH domain of epsin is involved in Cdc42 signaling regulation. Here, we present evidence that yeast epsin 2 (Ent2) plays a signaling role during cell division. We observed that overexpression of the ENTH domain of Ent2 (ENTH2), but not Ent1, promoted the formation of chains of cells and aberrant septa. This dominant-negative effect resulted from ENTH2-mediated interference with septin assembly pathways. We mapped the ENTH2 determinants responsible for induction of the phenotype and found them to be important for efficient binding to the septin regulatory protein, Bem3. Supporting a physiological role for epsin 2 in cell division, the protein localized to sites of polarized growth and cytokinesis and rescued a defect in cell division induced by Bem3 misregulation. Collectively, our findings provide a potential molecular mechanism linking endocytosis (via epsin 2) with signaling pathways regulating cell division.

Descripción: We consider first the zero-nonzero determination problem, which consists in determining the list of zero-nonzero conditions realized by a finite list of polynomials on a finite set ZâŠCk with C an algebraic closed field. We describe an algorithm to solve the zero-nonzero determination problem and we perform its bit complexity analysis. This algorithm, which is in many ways an adaptation of the methods used to solve the more classical sign determination problem, presents also new ideas which can be used to improve sign determination. Then, we consider the real-nonreal sign determination problem, which deals with both the sign determination and the zero-nonzero determination problem. We describe an algorithm to solve the real-nonreal sign determination problem, we perform its bit complexity analysis and we discuss this problem in a parametric context. © 2013 Elsevier Inc.

Descripción: Zero-range processes with jump rates that decrease with the number of particles per site can exhibit a condensation transition, where a positive fraction of all particles condenses on a single site when the total density exceeds a critical value. We consider rates which decay as a power law or a stretched exponential to a non-zero limiting value, and study the onset of condensation at the critical density. We establish a law of large numbers for the excess mass fraction in the maximum, as well as distributional limits for the fluctuations of the maximum and the fluctuations in the bulk. © 2013 Elsevier B.V. All rights reserved.

Descripción: In this work we present Zeus, a Distributed Model-Checker that evolves from the tool Kronos [8] and that currently can handle backwards computation of TCTL-reachability properties [1] over timed-automata [2]. Zeus was developed following a software architecture centric approach. It introduces some interesting features such as a priori graph partitioning, a sophisticated machinery to reach optimum performance (communication piggybacking and delayed messaging) and dead-time utilization, where every processor uses time intervals of inactivity to perform auxiliary, time-consuming tasks that will later speed up the rest of the computation. Although some good results have been obtained, early experiments pinpointed the difficulties of getting speedups using a parallel asynchronous version. We also propose some paths to overcome those obstacles. We would like to thank Sergio Yovine for making Kronos libraries available to us. © 2002 Published by Elsevier Science B.V.

Descripción: We examined the zooplankton abundance and composition of Laguna Grande, a floodplain wetland of the Lower Paraná Basin (Argentina), during an extraordinary drought-flood cycle that affected both the environment and the biological conditions of the lake. Low waters were characterised by remarkably high conductivities and pH values, and high phytoplankton and bacterioplankton abundances with cyanobacterial blooms, while high waters showed opposite features. In relation to zooplankton, the mean abundances of all the taxonomic groups (rotifers, cladocerans, copepods, ciliates, and heterotrophic nanoflagellates) were slightly higher at low waters. Major changes were observed in the specific composition of metazooplankton: the euryhaline species assemblage that dominated in the dry warm period was replaced by several oligohaline littoral and planktonic species characteristic of the Paraná River Basin, when the water level rose. Mean species richness values at high waters doubled those of low waters and were directly correlated to water depth. Most of the rotifers of the genus Brachionus and the cladoceran Moina micrura switched from parthenogenetic to sexual reproduction during low waters, as a response to a harsh environment and crowding. We suggest that the main changes in the environmental conditions in this eutrophic floodplain lake are driven by the hydrology, which regulates the zooplankton succession. The herein described shifts in the zooplankton structure and dynamics of Laguna Grande over an extraordinary drought-flood cycle contribute to the understanding of the processes that might occur under the scenarios predicted by climate change models. © 2011 Elsevier GmbH.