Descripción: The correlation between the ontogeny of Ubisch bodies and pollen development in Oxalis articulata was studied with Transmission Electron Microscopy (TEM). The ultrastructural changes observed during the different stages of development in the tapetal cells are related to Ubisch bodies, sporopollenin and pollen-kitt formation. The pro-orbicules have the appearance of lipid globuli and their formation is related to the endoplasmic reticulum of rough type (ERr). The lipid globules or pro-orbicules disappear in the mature Ubisch bodies, and the places that they occupied remain free of contents or with pollen-kitt.

Descripción: The Saccharomyces cerevisiae UGA4 gene encodes a permease capable of importing γ-aminobutyric acid (GABA) and δ-aminolevulinic acid (ALA) into the cell. GABA-dependent induction of this permease requires at least two positive-acting proteins, the specific factor Uga3 and the pleiotropic factor Uga35/Dal81. UGA4 is subjected to a very complex regulation, and its induction is affected by the presence of extracellular amino acids; this effect is mediated by the plasma membrane amino acid sensor SPS. Our results show that leucine affects UGA4 induction and that the SPS sensor and the downstream effectors Stp1 and Stp2 participate in this regulation. Moreover, we found that the Uga3 and Uga35/Dal81 transcription factors bind to the UGA4 promoter in a GABA-dependent manner and that this binding is impaired by the presence of leucine. We also found that the Leu3 transcription factor negatively regulates UGA4 transcription, although this seems to be through an indirect mechanism. © 2010, American Society for Microbiology.

Descripción: The lung of the toad, Melanophryniscus stelzneri stelzneri was studied using scanning and transmission electron microscopy. In M.s.stelzneri the parenchyma forms a polygonal network arrangement, therefore the parenchyma is edicular. These spaces are delimited by the interconnection of third order septa which are covered by respiratory epithelium. Small patches of ciliated epithelium without goblet cells appear irregularly distributed on the septa. The respiratory epithelium consists of one type of pneumocyte, which shows characteristics of both type I and type II alveolar cells of higher vertebrates. The pneumocytes are irregular in shape and possess attenuated cytoplasmic processes, which spread around the capillaries to form the outer layer of the air-blood barrier. These cells contain different types of cytoplasmic bodies: electron dense bodies, multivesicular bodies and lamellar bodies. Dense bodies are probably the precursors of lamellar bodies and the multivesicular bodies are incorporated into the latter. Neuroepithelial bodies appear randomly distributed over the septa. These bodies are separated from the lumen of the lung by thin cytoplasmic processes of neighbouring pneumocytes. The air-blood barrier consists of three layers: epithelium, interstitial space and endothelium. The relatively simple pulmonary structure of M.s.stelzneri is due to a lower degree of partitioning of the pulmonary lumen in comparison to the lung of other bufonid anurans, could be correlated with a well developed cutaneous and buccopharingeal respiration. The testing of this hypothesis awaits further studies.

Descripción: The sperm morphology of Adelomelon beckii is described by optical and transmission electron microscopy. Both euspermatozoa and paraspermatozoa were found in the specimens studied. Euspermatozoa are filiform and have an elongate nucleus capped by an acrosome. A small basal plate lies between the base of the acrosome and the nucleus. The mid-piece consists of U-shaped mitochondria wrapped helically around the central axoneme. A dense annulus at the junction of the mid-piece and glycogen piece is found, ending in a short end-piece, composed of the axoneme surrounded by a plasma membrane. Two types of paraspermatozoa are found, both vermiform but differing internally with respect to the disposition and number of axonemes, as well as to the types of secretory vesicles. We suggest the use of paraspermatozoa as a systematic character to reveal phylogenetic relationships in this family. © 2009 Springer-Verlag and AWI.

Descripción: Background: Alzheimer's disease (AD) is characterized by a neurodegenerative progression that alters cognition. On a phenotypical level, cognition is evaluated by means of the MiniMental State Examination (MMSE) and the post-morten examination of Neurofibrillary Tangle count (NFT) helps to confirm an AD diagnostic. The MMSE evaluates different aspects of cognition including orientation, short-term memory (retention and recall), attention and language. As there is a normal cognitive decline with aging, and death is the final state on which NFT can be counted, the identification of brain gene expression biomarkers from these phenotypical measures has been elusive. Methodology/Principal Findings: We have reanalysed a microarray dataset contributed in 2004 by Blalock et al. of 31 samples corresponding to hippocampus gene expression from 22 AD subjects of varying degree of severity and 9 controls. Instead of only relying on correlations of gene expression with the associated MMSE and NFT measures, and by using modern bioinformatics methods based on information theory and combinatorial optimization, we uncovered a 1,372-probe gene expression signature that presents a high-consensus with established markers of progression in AD. The signature reveals alterations in calcium, insulin, phosphatidylinositol and wnt-signalling. Among the most correlated gene probes with AD severity we found those linked to synaptic function, neurofilament bundle assembly and neuronal plasticity. Conclusions/Significance: A transcription factors analysis of 1,372-probe signature reveals significant associations with the EGR/KROX family of proteins, MAZ, and E2F1. The gene homologous of EGR1, zif268, Egr-1 or Zenk, together with other members of the EGR family, are consolidating a key role in the neuronal plasticity in the brain. These results indicate a degree of commonality between putative genes involved in AD and prion-induced neurodegenerative processes that warrants further investigation. © 2010 Go ́mez Ravetti et al.

Descripción: The effect of donor ligands, usually expected to enhance the reactivity of organolithiums, was studied on the addition of PhLi to E-cinnamaldehyde in THF, under conditions that lead the reaction toward the production of 1,3-diphenylpropanone. It was observed that in the presence of TMEDA and HMPT, the rate of that reaction becomes slower than in the absence of ligands; the effect of HMPA was even more spectacular, at concentration [HMPA]: [PhLi] ≥ 4 the reaction becomes almost completely inhibited. These results show the complexity of solvation effects on the reactions of organolithiums and how substrate-reagent-ligand-solvent interactions are usually specific for the system under study. © ARKAT.

Descripción: Phasins (PhaP) are proteins normally associated with granules of poly(3-hydroxybutyrate) (PHB), a biodegradable polymer accumulated by many bacteria as a reserve molecule. These proteins enhance growth and polymer production in natural and recombinant PHB producers. It has been shown that the production of PHB causes stress in recombinant Escherichia coli, revealed by an increase in the concentrations of several heat stress proteins. In this work, quantitative reverse transcription (qRT)-PCR analysis was used to study the effect of PHB accumulation, and that of PhaP from Azotobacter sp. strain FA8, on the expression of stress-related genes in PHB-producing E. coli. While PHB accumulation was found to increase the transcription of dnaK and ibpA, the expression of these genes and of groES, groEL, rpoH, dps, and yfiD was reduced, when PhaP was coexpressed, to levels even lower than those detected in the non-PHB-accumulating control. These results demonstrated the protective role of PhaP in PHB-synthesizing E. coli and linked the effects of the protein to the expression of stress-related genes, especially ibpA. The effect of PhaP was also analyzed in non-PHBsynthesizing strains, showing that expression of this heterologous protein has an unexpected protective effect in E. coli, under both normal and stress conditions, resulting in increased growth and higher resistance to both heat shock and superoxide stress by paraquat. In addition, PhaP expression was shown to reduce RpoH protein levels during heat shock, probably by reducing or titrating the levels of misfolded proteins. © 2011, American Society for Microbiology.

Descripción: Animals, from invertebrates to humans, stabilize the panoramic optic flow through compensatory movements of the eyes, the head or the whole body, a behavior known as optomotor response (OR). The same optic flow moved clockwise or anticlockwise elicits equivalent compensatory right or left turning movements, respectively. However, if stimulated monocularly, many animals show a unique effective direction of motion, i.e., a unidirectional OR. This phenomenon has been reported in various species from mammals to birds, reptiles, and amphibious, but among invertebrates, it has only been tested in flies, where the directional sensitivity is opposite to that found in vertebrates. Although OR has been extensively investigated in crabs, directional sensitivity has never been analyzed. Here, we present results of behavioral experiments aimed at exploring the directional sensitivity of the OR in two crab species belonging to different families: the varunid mud crab Neohelice granulata and the ocypode fiddler crab Uca uruguayensis. By using different conditions of visual perception (binocular, left or right monocular) and direction of flow field motion (clockwise, anticlockwise), we found in both species that in monocular conditions, OR is effectively displayed only with progressive (front-to-back) motion stimulation. Binocularly elicited responses were directional insensitive and significantly weaker than monocular responses. These results are coincident with those described in flies and suggest a commonality in the circuit underlying this behavior among arthropods. Additionally, we found the existence of a remarkable eye dominance for the OR, which is associated to the size of the larger claw. This is more evident in the fiddler crab where the difference between the two claws is huge. Copyright © 2019 Barnatan, Tomsic and Sztarker. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Descripción: We investigate an interacting two-fluid cosmological model and introduce a scalar field representation by means of a linear combination of the individual energy densities. Applying the integrability condition to the scalar field equation we show that this "exotic quintessence" is driven by an exponential potential and the two-fluid mixture can be considered as a model of three components. These components are associated with baryonic matter, dark matter and dark energy respectively. We use the Simon, Verde and Jimenez [J. Simon, L. Verde, R. Jimenez, Phys. Rev. D 71 (2005) 123001] determination of the redshift dependence of the Hubble parameter to constrain the current density parameters of this model. With the best fit density parameters we obtain the transition redshift between non-accelerated and accelerated regimes zacc = 0.66 and the time elapsed since the initial singularity t0 = 19.8 Gyr. We study the perturbation evolution of this model and find that the energy density perturbation decreases with the cosmological time. © 2008 Elsevier B.V. All rights reserved.

Descripción: The uniqueness of the energy momentum tensor in non-Abelian gauge field theories is established under minimal hypothesis. © 1989 American Institute of Physics.

Descripción: We study formal deformations of a crossed product S(V)#fG, of a polynomial algebra with a group, induced from a universal deformation formula introduced by Witherspoon. These deformations arise from braided actions of Hopf algebras generated by automorphisms and skew derivations. We show that they are non-trivial in the characteristic free context, even if G is infinite, by showing that their infinitesimals are not coboundaries. For this we construct a new complex which computes the Hochschild cohomology of S(V)#fG. © 2011 Elsevier Inc.

Descripción: Data collected 10 years ago in Argentina concluded that women in physics were underrepresented in many instances and that a «crystal ceiling» was firmly in place. We have collected updated data for several indicators and compared them with those obtained 10 years ago. Although there is not a clear conclusion to be drawn from this comparison, we try to explain the results within the framework of the changes in scientific policies in Argentina.© 2013 American Institute of Physics.

Descripción: A composite section, 1400 m thick, is established for the Upper Cretaceous-Paleogene strata in the fold and thrust belt of the Andes Fueguinos, south of 54° 30′S. The basal Policarpo Formation, > 350 m thick, consists of tuffaceous sandstones and bioturbated sandstones and siltstones. The ammonites Maorites densicostatus and Diplomoceras sp.; foraminifera common to the Gaudryina healyi Zone; and the dynocysts Manumiella seelandica and Operculodium cf. azcaratei indicate a Maastrichtian age. The Paleocene to lower Eocene include four new formations. The Cabo Leticia Formation, c.150 m, Paleocene, consists of gravity flows deposits: brecchias; conglomerates; and massive, tuffaceous sandstones. La Barca Formation, c. 220 m, includes two members: LB1, tuffaceous sandstones and intercalated carbonaceous siltstones; and LB2, black mudstones with Palaeocystodinium golzowense and Spiroplectammina spectabilis. P. golzowense and the Bulimina karpatica Assemblage in LB1 indicate a late Paleocene age. Punta Noguera Formation, 380 m, is dominated by glauconite rich, massive, tuffaceous sandstones with interbedded turbidite packages. The dynocyst group Apectodinium, Deflandrea robusta, Palaeocystodinium sp., and Odontodinium askinae; the foraminifera Alabamina creta, Charltonina acutimarginata, Valvulineria teurensis and the first ocurrence of Elphidium and Cribrorotalia suggest an age near the Paleocene/Eocene boundary. The Cerro Ruperto Formation, 200 m, is dominated by glauconite rich, silty very fine sandstones and siltstones; dominance of Deflandrea dartmooria indicate an early Eocene age. Resting on angular unconformity, the Río Bueno Formation, c. 60-80 m, consists of carbonate rocks; its member RB1, rhythmically bedded grainstones, with the planktic foraminifera Planorotalites australiformis and Subbotina linaperta is of early middle Eocene age; and its member RB2, regular alternation of grainstones and bioturbated marls and micrites, with Elphidium saginatum and Bulimina cf. bortonica, is assigned to the mid middle Eocene. The sedimentary and stratigraphic features of the Maastrichtian/Eocene have a strong tectonic control, suggesting that the foreland phase of evolution of the basin has been active at least since the Maastrichtian.

Descripción: Leptin, the 16,000 molecular weight protein product of the obese gene, was originally considered as an adipocyte-derived signaling moleculeforthe central control of metabolism. However, leptin has been suggested to be involved in other functions during pregnancy, particularly in placenta, in which it was found to be expressed. In the present work, we have found that recombinant human chorionic gonadotropin (hCG) added to BeWo choriocarcinoma cell line showed a stimulatory effect on endogenous leptin expression, when analyzed by Western blot. This effect was time and dose dependent. Maximal effect was achieved at hCG 100 IU/ml. Moreover, hCG treatment enhanced leptin promoter activity up to 12.9 times, evaluated by transient transfection with a plasmid construction containing different promoter regions and the reporter gene luciferase. This effect was dose dependent and evidenced with all the promoter regions analyzed, regardless of length. Similar results were obtained with placental explants, thus indicating physiological relevance. Because hCG signal transduction usually involves cAMP signaling, this pathway was analyzed. Contrarily, we found that dibutyryl cAMP counteracted hCG effect on leptin expression. Furthermore, cotransfection with the catalytic subunit of PKA and/or the transcription factor cAMP response element binding protein repressed leptin expression. Thereafter we determined that hCG effect could be partially blocked by pharmacologic inhibition of MAPK pathway with 50 μM PD98059 but not by the inhibition of the phosphatidylinositol 3-kinase pathway with 0.1 μm wortmannin. Moreover, hCG treatment promoted MAPK kinase and ERK1/ERK2 phosphorylation in placental cells. Finally, cotransfection with a dominant-negative mutant of MAPK blocked the hCG-mediated activation of leptin expression. In conclusion, we provide some evidence suggesting that hCG induces leptin expression in trophoblastic cells probably involving the MAPK signal transduction pathway. Copyright © 2009.

Descripción: The main environmental problems existing in Buenos Aires metropolitan area were studied in an area located on the Pampean plains (Llanura Pampeana) at about 32°S and 66°W. Since its foundation in 1580, the city of Buenos Aires has experienced a striking population growth, to its present level of 13,000,000 inhabitants. The main geoenvironmental problems that pose a threat to Buenos Aires population are: flooding, regional rise in the water table, disposal of domestic and industrial waste in land-fill sites, mining dust, expansive soils and contamination of soils, water and air. This paper focuses on the geological, geomorphological and edaphic aspects, which may have controlled the occurrence of these processes. Thematic mapping of the main physical environmental features would appear to be one of the main tools for solving these problems. © 2004 Asociación Geológica Argentina.

Descripción: The purpose of the paper is to analyze the geomorphology and hydrodynamic of the coastal area, to model the evolution of the coastal cohesive cliffs and the handing dune barrier system along southern Buenos Aires coast. The morphology and evolution of coastal transverse profile are used as geoindicator of erosional susceptibility for planning and manage the coastal communities' development. The vulnerability to erosion increases from dune coasts, to stabilized dunes, cliffs covered with ramps, cliffs with ramps, cliffs with mixed ramps, inactive cliffs and active cliffs. Human intervention such as afforestation, urbanization, mining and interruption of the littoral drift by Quequen harbor groins, have altered the eolian and marine sand supply in the area, modifying the coastal profile configuration, trending to the disappearance of sand ramps.

Descripción: The use of the magnetic signal of soils and paleosoils as a climatic function. The correlation between an index of potential water storage (PWS) with magnetic signal in soils and paleosoils, developed in loessic sediments has been proposed. The analyzed data suggest the existence of climatic thresholds that affect the genesis, preservation or depletion of ferrimagnetic minerals. Soils and paleosoils characterized by a positive PWS have an appropriate environment that favors the depletion of ferromagnetic minerals due to mainly reductive loss. Such soils are characterized by a depletion of detrital ferrimagnetic minerals, as in northeastern Pampean plain soils and paleosoils of Argentina and SE of China. A negative PWS prevents highly reducing conditions in the soil, and the detrital ferrimagnetic minerals are preserved. On the other hand, the environmental conditions of these soils allow the formation of new nannoparticle minerals. These conditions produce a net magnetic enhancement of the soil, as observed in European soils (Russia, Czech Republic, Rumania), Asian (paleosoils from the loess plateau of China) and Northern African ones (Tunisia). The influence of parent material in the magnetic signal is also discussed.

Descripción: Text information in images and videos is frequently a key factor for information indexing and retrieval systems. However, text detection in images is a difficult task since it is often embedded in complex backgrounds. In this paper, we propose an accurate text detection and localization method in images based on stroke information and the Adaptive Run Lenght Smoothing Algorithm. Experimental results show that the proposed approach is accurate, has high recall and is robust to various text sizes, fonts, colors and languages. © 2011 Springer-Verlag.

Descripción: Dendritic cell (DC) and natural killer (NK) cell interactions are important for the regulation of innate and adaptive immunity, but their relevance during early pregnancy remains elusive. Using two different strategies to manipulate the frequency of NK cells and DC during gestation, we investigated their relative impact on the decidualization process and on angiogenic responses that characterize murine implantation. Manipulation of the frequency of NK cells, DC or both lead to a defective decidual response characterized by decreased proliferation and differentiation of stromal cells. Whereas no detrimental effects were evident upon expansion of DC, NK cell ablation in such expanded DC mice severely compromised decidual development and led to early pregnancy loss. Pregnancy failure in these mice was associated with an unbalanced production of anti-angiogenic signals and most notably, with increased expression of genes related to inflammation and immunogenic activation of DC. Thus, NK cells appear to play an important role counteracting potential anomalies raised by DC expansion and overactivity in the decidua, becoming critical for normal pregnancy progression. © 2012 González et al.

Descripción: Each day, advances in the instrumentation and operating protocols bring new applications and insights into the molecular processes of ultra violet-matrix assisted laser desorption/ionization-mass spectrometry (UV-MALDI MS), increasing its potential use. We report here an approach in which mass spectrometry analysis of sphingolipids has been performed using a fluorescent tag (nitrobenz-2-oxa-1, 3-diazole, NBD) covalently linked to the sphingoid base as matrix. Thus, different labeled-sphingolipids were analyzed: ceramide, dihydroceramide, acetylceramide, glucosylceramide, galactosylceramide, galactosyldihydroceramide. In addition an extract of glycosphingolipids obtained from epimastigote forms of Trypanosoma cruzi metabolically labeled with NBD-ceramide was analyzed. The goal of this work is to show that no matrix needs to be added for the mass spectrometry analysis as the same tag used to label the lipids may generate efficiently analyte ions to obtain high quality signals. © 2008 American Society for Mass Spectrometry.

Descripción: Nitrous acid degradation of heparin followed by high-performance anion-exchange chromatography (HPAEC) separation and ultraviolet matrix assisted laser desorption/ionization time-of-flight (UV-MALDI-TOF) analysis led to the structural determination of six sulfated oligosaccharides. Three different matrices (α-cyano-4-hydroxycinnamic acid (CHCA), nor-harmane, and dihydroxybenzoic acid (DHB)) have been used, and the complementary results obtained allowed in most cases to assign the position of sulfate groups. Based on the different cleavages produced on the purified oligosaccharides in source during the MS analysis by the use of the different matrices, this approach provides a new tool for structural analysis. © 2010 American Society for Mass Spectrometry.