Descripción: 17O chemical shifts are found to be highly sensitive probes in the study of keto-enol tautomeric equilibria due to the strong sensitivity of these shifts to the coordination of the oxygen atom. The large shielding effect observed, both experimentally and theoretically, for the carbonyl oxygen atom in 2-pyridone suggests that the carbonyl π-electronic system is undergoing a strong conjugation with the formal C3=C4 double bond and/or the nitrogen lone pair. A Cl or NH2 substitution at position 6 of the pyridine ring shifts the tautomeric equilibrium toward the 2-hydroxypyridine form, while a CH3 substitution results in the keto form being predominant, as is the case in the parent compound. © 1992 American Chemical Society.

Descripción: The 17O chemical shifts of several ortho-substituted phenols and anisoles are presented along with those of the corresponding para-substituted compounds. The measurements of the various ortho-para pairs were done in the same solvent and at the same temperature in order to experimentally estimate the effect of intramolecular hydrogen bonding on the 17O chemical shielding in the ortho compounds by using the ortho-para equivalence rule. Ab initio calculations of the 17O chemical shieldings reproduce well the experimental findings and provide a theoretical description of the hydrogen-bonding effect on the 17O chemical shifts. © 1991 American Chemical Society.

Descripción: The conventional random phase approximation (RPA) of the polarization propagator theory and a computational method based on continuous transformation of origin for the current density (CTOCD) induced within the electron cloud by an external homogeneous, static magnetic field has been employed to calculate atomic contributions to magnetic susceptibilities. The diamagnetic part of the magnetic susceptibility is written in terms of the polarization propagator. Since the paramagnetic term may also be obtained from the propagator it is thus possible to compute both contributions at the same level of approximation. The evaluated average susceptibility is independent of the origin of the vector potential, but depends on the origin of the reference frame. The atomic contributions to the diamagnetic and paramagnetic parts of the magnetic susceptibility are derived by applying off-diagonal hypervirial relations which are exactly fulfilled if the state functions are exact eigenfunctions of a model Hamiltonian. The rationalization of the magnetic susceptibilities into atomic contributions is applied to some small molecules: HF, H2O, NH3 and CH4, and the sum of these contributions is compared to the corresponding calculated total values and the experimental data for the molecular magnetic susceptibility for the same compounds. Computations are performed using basis sets of increasing quality. A series of sum rules for gauge independence of the computed results and charge-current conservation have been tested to document the accuracy of the calculation of magnetic properties. © 1999 American Institute of Physics.

Descripción: A novel method for simulating the statistical mechanics of molecular systems in which both nuclear and electronic degrees of freedom are treated quantum mechanically is presented. The scheme combines a path integral description of the nuclear variables with a first-principles adiabatic description of the electronic structure. The electronic problem is solved for the ground state within a density functional approach, with the electronic orbitals expanded in a localized (Gaussian) basis set. The discretized path integral is computed by a METROPOLIS Monte Carlo sampling technique on the normal modes of the isomorphic ring polymer. An effective short-time action correct to order τ4 is used. The validity and performance of the method are tested by studying two small lithium clusters, namely Li4 and Li5+. Structural and electronic properties computed within this fully quantum-mechanical scheme are presented and compared to those obtained within the classical nuclei approximation. Quantum delocalization effects turn out to be significant as shown by the fact that quantum simulation results at 50 K approximately correspond to those of classical simulations carried out at 150 K. The scaling factor depends, however, on the specific physical property, thus evidencing the different character of quantum and thermal correlations. Tunneling turns out to be irrelevant in the temperature range investigated (50-200 K). © 1998 American Institute of Physics.

Descripción: Munida gregaria and M. subrugosa have been considered two different species for more than a century; however, after a recent molecular phylogenetic study, they are considered a single polymorphic species. Yet, the use of markers to diagnose species may be misleading when divergence between species is recent, since a speciation event may be obscured by the retention and stochastic sorting of ancestral polymorphisms. The morphs gregaria and subrugosa of Munida gregaria constitute an interesting case for the study of behavioural isolation since they are sympatric, breed at the same time of the year, and might have experienced a recent speciation. Mating behaviour observations and mate choice mating trials were conducted in order to investigate the potential existence of a behavioural prezygotic barrier to gene flow between these two morphs. Since factors involved in mate choice in galatheids are unknown, the four possible combinations of the two different morphs in trios were used to test for the existence of mate choice. Video recordings of all the possible trio combinations revealed that there was cross-attraction between males and females of different morphs. Females bearing partial broods participated in encounters as well as non-ovigerous females. The frequency and duration of homo- and heterotypic encounters were registered, and a reproductive isolation index was calculated for each variable for each trio. The isolation indexes calculated were not different from zero indicating random mating, and were not affected by the composition of the trio or the partial ovigerous condition of females. These results provided evidence of the absence of behavioural prezygotic barriers to gene flow between the morphs gregaria and subrugosa of M. gregaria. © 2011 Springer-Verlag and AWI.

Descripción: The electroencephalogram (EEG) together with electromyogram (EMG) of the ischiocavernosus, bulbocavernosus and levator penis muscles were chronically monitored across behavioral states of the armadillo Chaetophractus villosus. This animal has a very long penis, which exhibits remarkable phenomena during wakefulness (W), slow wave sleep (SWS) and paradoxical sleep (PS). During W it remains retracted within a skin receptacle. During SWS penile protrusion can be observed together with very complex movements. Protrusion is a non erectile event during which the penis remains out of its receptacle but without rigidity. Penile erections are observed only during SWS. Contrasting with other mammals, no erections occur during PS. During this phase the penile muscles share the atonia of the body musculature characteristic of that phase. Some reflections on mechanisms of those penile events are presented.

Descripción: Orchestrators are descriptions at implementation level and may contain sensitive information that should be kept private. Consequently, orchestration languages come equipped with a notion of abstract processes, which enable the interaction among parties while hiding private information. An interesting question is whether an abstract process accurately describes the behavior of a concrete process so to ensure that some particular property is preserved when composing services. In this paper we focus on compliance, i.e, the correct interaction of two orchestrators and we introduce two definitions of abstraction: one in terms of traces, called trace-based abstraction, and the other as a generalization of symbolic bisimulation, called simulation-based abstraction.We show that simulation-based abstraction is strictly more refined than trace-based abstraction and that simulation-based abstraction behaves well with respect to compliance.

Descripción: Ciliates from sub-surface waters of the Argentine shelf and the Drake Passage under austral summer and autumn conditions were examined and compared for the first time. In both environments, the taxonomic structure of ciliates was related to temperature and salinity, and aloricate oligotrichs dominated in density (80%) over loricate oligotrichs, litostomatids and prostomatids, while the microplanktonic fraction prevailed in terms of biomass (90%) over the nanociliates. Myrionecta rubra was found all along the Argentine shelf only in autumn, but showed isolated peaks of abundance (103 ind. L -1) during summer. Mean values of density and biomass of total ciliates decreased ca. 2-fold from the shelf-slope to oceanic waters, while potential maximum production of aloricate oligotrichs decreased 9-fold, in relation with the drop in chlorophyll a concentration and the latitudinal decline of temperature, also reflected in maximum growth rates. Fifty percent of total ciliate abundance was represented by local increases (maximum: 20 000 ind. L-1 and 25 μg C L-1), which were spatially superimposed with ranges of seawater temperature and chlorophyll a concentrations of 10-15°C and 0.6-6 μg L-1, respectively, and were found in the nearby of fronts located on the shelf and the slope.

Descripción: Whether or not supernovae contribute significantly to the overall dust budget is a controversial subject. Submillimetre (sub-mm) observations, sensitive to cold dust, have shown an excess at 450 and 850 μm in young remnants Cassiopeia A (Cas A) and Kepler. Some of the sub-mm emission from Cas A has been shown to be contaminated by unrelated material along the line of sight. In this paper, we explore the emission from material towards Kepler using sub-mm continuum imaging and spectroscopic observations of atomic and molecular gas, via H i, 12CO(J = 2-1) and 13CO(J = 2-1). We detect weak CO emission (peak T* A = 0.2-1 K, 1-2 km s -1 full width at half-maximum) from diffuse, optically thin gas at the locations of some of the sub-mm clumps. The contribution to the sub-mm emission from foreground molecular and atomic clouds is negligible. The revised dust mass for Kepler's remnant is 0.1-1.2 M ⊙, about half of the quoted values in the original study by Morgan et al., but still sufficient to explain the origin of dust at high redshifts. © 2009 RAS.

Descripción: Complex-valued functionsf1,...,fronRdarerefinableif they are linear combinations of finitely many of the rescaled and translated functionsfi(Ax-k), where the translateskare taken along a latticeΓ⊂RdandAis adilation matrixthat expansively mapsΓinto itself. Refinable functions satisfy arefinement equationf(x)=∑k∈Λckf(Ax-k), whereΛis a finite subset ofΓ, theckarer×rmatrices, andf(x)=(f1(x),...,fr(x))T. Theaccuracyoffis the highest degreepsuch that all multivariate polynomialsqwith degree(q)<pare exactly reproduced from linear combinations of translates off1,...,fralong the latticeΓ. In this paper, we determine the accuracypfrom the matricesck. Moreover, we determine explicitly the coefficientsyα,i(k) such thatxα=∑ri=1∑ k∈Γyα,i(k)fi(x+k). These coefficients are multivariate polynomialsyα,i(x) of degree α evaluated at lattice pointsk∈1. © 1998 Academic Press.

Descripción: Acetoacetyl-CoA thiolase (EC 2.3.1.9), also called thiolase II, condenses two molecules of acetyl-CoA to give acetoacetyl-CoA. This is the first enzymatic step in the biosynthesis of isoprenoids via mevalonate (MVA). In this work, thiolase II from alfalfa (MsAACT1) was identified and cloned. The enzymatic activity was experimentally demonstrated in planta and in heterologous systems. The condensation reaction by MsAACT1 was proved to be inhibited by CoA suggesting a negative feedback regulation of isoprenoid production. Real-time RT-PCR analysis indicated that MsAACT1 expression is highly increased in roots and leaves under cold and salinity stress. Treatment with mevastatin, a specific inhibitor of the MVA pathway, resulted in a decrease in squalene production, antioxidant activity, and the survival of stressed plants. As expected, the presence of mevastatin did not change chlorophyll and carotenoid levels, isoprenoids synthesized via the plastidial MVA-independent pathway. The addition of vitamin C suppressed the sensitive phenotype of plants challenged with mevastatin, suggesting a critical function of the MVA pathway in abiotic stress-inducible antioxidant defence. MsAACT1 over-expressing transgenic plants showed salinity tolerance comparable with empty vector transformed plants and enhanced production of squalene without altering the 3-hydroxy-3-methylglutaryl- CoA reductase (HMGR) activity in salt-stress conditions. Thus, acetoacetyl-CoA thiolase is a regulatory enzyme in isoprenoid biosynthesis involved in abiotic stress adaptation. © 2011 The Author.

Descripción: Background: Resolving the kinetics of agonist binding events separately from the subsequent channel gating processes requires the ability of applying and removing the agonist before channel gating occurs. No reported system has yet achieved pulses shorter than 100 μs, necessary to study nicotinic ACh receptor or AMPA receptor activation. Methodology/Principal Findings: Solution exchange systems deliver short agonist pulses by moving a sharp interface between a control and an experimental solution across a channel preparation. We achieved shorter pulses by means of an exchange system that combines a faster flow velocity, narrower partition between the two streams, and increased velocity and bandwidth of the movement of the interface. The measured response of the entire system was fed back to optimize the voltage signal applied to the piezoelectric actuator overcoming the spurious oscillations arising from the mechanical resonances when a high bandwidth driving function was applied. Optimization was accomplished by analyzing the transfer function of the solution exchange system. When driven by optimized command pulses the enhanced system provided pulses lasting 26 ± 1 μs and exchanging 93 ± 1% of the solution, as measured in the open tip of a patch pipette. Conclusions/Significance: Pulses of this duration open the experimental study of the molecular events that occur between the agonist binding and the opening of the channel. © 2012 Auzmendi et al.

Descripción: Real time detection of pulsed laser surface melting was performed by analyzing the photoacoustic signals produced on the samples. Comparison between the amplitudes of the transversal and longitudinal waves allowed us to identify the fluence thresholds for surface melting. The method was tested with AISI 304 stainless steel samples and the results obtained were checked against direct metallographic analysis. © 1997 American Institute of Physics.

Descripción: Nur factors are critical for proopiomelanocortin (POMC) induction by CRH in corticotrophs, but the pathways linking CRH to Nur are unknown. In this study we show that in AtT-20 corticotrophs CRH and cAMP induce Nur77 and Nurr1 expression and transcription at the NurRE site by protein kinase A (PKA) and calcium-dependent and -independent mechanisms. Calcium pathways depend on calmodulin kinase II (CAMKII) activity, and calcium-independent pathways are accounted for in part by MAPK activation (Rap1/B-Raf/MAPK-ERK kinase/ERK1/2), demonstrated by the use of molecular and pharmacological tools. ATT-20 corticotrophs express B-Raf, as do other cells in which cAMP stimulates MAPK. CRH/cAMP stimulated ERK2 activity and increased transcriptional activity of a Gal4-Elk1 protein, which was blocked by overexpression of dominant negative mutants and kinase inhibitors and stimulated by expression of B-Raf. The MAPK kinase inhibitors did not affect Nur77 and Nurr1 mRNA induction but blocked CRH or cAMP-stimulated Nur transcriptional activity. Moreover, MAPK stimulated phosphorylation and transactivation of Nur77. The functional impact of these pathways was confirmed at the POMC promoter. In conclusion, in AtT-20 corticotrophs the CRH/cAMP signaling that leads to Nur77/Nurr1 mRNA induction and transcriptional activation, and thus POMC expression, is dependent on protein kinase A and involves calcium/calmodulin kinase II (Nur induction/activation) and MAPK calcium-dependent and -independent (Nur phosphorylation-activation) pathways.

Descripción: The present study focused on interactions between signaling pathways activated by progestins and by type I and II receptor tyrosine kinases (RTKs) in mammary tumors. An experimental model in which the synthetic progestin medroxyprogesterone acetate (MPA) induced mammary adenocarcinomas in Balb/c mice was used. MPA-stimulated proliferation, both in vivo and in vitro, of progestin-dependent tumors induced up-regulation of ErbB-2 protein levels and tyrosine phosphorylation of this receptor. Combinations of antisense oligodeoxynueleotides (ASODNs) directed to ErbB-2 mRNA with ASODNs directed to the insulin-like growth factor-I receptor (IGF-IR) were used to study the effect of the simultaneous block of these receptors on the MPA-induced proliferation of epithelial cells from the progestin-dependent C4HD line. Neither synergistic nor additive effects on the inhibition of MPA-induced proliferation of C4HD cells were observed as a result of the combination of these ASODNs. Suppression of IGF-IR expression by ASODNs resulted in complete abrogation of MPA-induced phosphorylation of ErbB-2 in C4HD cells, whereas blockage of ErbB-2 did not affect IGF-IR phosphorylation. These results show the existence of a hierarchical interaction between IGF-IR and ErbB-2, by means of which IGF-IR directs ErbB-2 phosphorylation. We demonstrated, for the first time, that this hierarchical interaction involves physical association of both receptors, resulting in the formation of a heteromeric complex. Furthermore, confocal laser microscopy experiments demonstrated that MPA was able to induce co-localization of ErbB-2 and IGF-IR. This hetero-oligomer was also found in MCF-7 human breast cancer cells in which association of IGF-IR and ErbB-2 was induced by heregulin and IGF-I.

Descripción: Initially, memory is labile and requires consolidation to become stable. However, several studies support that consolidated memories can undergo a new period of lability after retrieval. The mechanistic differences of this process, termed reconsolidation, with the consolidation process are under debate, including the participation of hippocampus. Up to this point, few reports describe molecular changes and, in particular, transcription factor (TF) involvement in memory restabilization. Increasing evidence supports the participation of the TF nuclear factor-κB (NF-κB) in memory consolidation. Here, we demonstrate that the inhibition of NF-κB after memory reactivation impairs retention of a hippocampal-dependent inhibitory avoidance task in mice. We used two independent disruptive strategies to reach this conclusion. First, we administered intracerebroventricular or intrahippocampal sulfasalazine, an inhibitor of IKK (IκB kinase), the kinase that activates NF-κB. Second, we infused intracerebroventricular or intrahippocampal κB decoy, a direct inhibitor of NF-κB consisting of a double-stranded DNA oligonucleotide that contains the κB consensus sequence. When injected immediately after memory retrieval, sulfasalazine or κB decoy (Decoy) impaired long-term retention. In contrast, a one base mutated κB decoy (mDecoy) had no effect. Furthermore, we also found NF-κB activation in the hippocampus, with a peak 15 min after memory retrieval. This activation was earlier than that found during consolidation. Together, these results indicate that NF-κB is an important transcriptional regulator in memory consolidation and reconsolidation in hippocampus, although the temporal kinetics of activation differs between the two processes. Copyright © 2007 Society for Neuroscience.

Descripción: Several studies support that stored memories undergo a new period of consolidation after retrieval. It is not known whether this process, termed reconsolidation, requires the same transcriptional mechanisms involved in consolidation. Increasing evidence supports the participation of the transcription factor NF-κB in memory. This was initially demonstrated in the crab Chasmagnathus model of associative contextual memory, in which re-exposure to the training context induces a well characterized reconsolidation process. Here we studied the role of NF-κB in reconsolidation. NF-κB was specifically activated in trained animals re-exposed to the training context but not to a different context. NF-κB was not activated when animals were re-exposed to the context after a weak training protocol insufficient to induce long-term memory. A specific inhibitor of the NF-κB pathway, sulfasalazine, impaired reconsolidation when administered 20 min before re-exposure to the training context but was not effective when a different context was used. These findings indicate for the first time that NF-κB is activated specifically by retrieval and that this activation is required for memory reconsolidation, supporting the view that this molecular mechanism is required in both consolidation and reconsolidation.

Descripción: Split lamellae of posterior gills of Chasmagnathus granulatus adapted to 2.5‰ salinity were mounted in a modified Ussing chamber. With NaCl-saline on both sides of the preparation a transepithelial voltage (Vte) of 4.1±0.5 mV (outside positive) was measured. After voltage-clamping, the negative short-circuit current (Isc) amounted to -142±21 μA cm-2 at a conductance (Gte) of 44±5 mS cm-2. Substitution of either chloride (by nitrate) or sodium (by choline) on both sides of split gill lamellae significantly reduced Isc (by 70-80%) and Gte (by 30-50%). External CsCl (but not BaCl2 or furosemide) inhibited the negative Isc without affecting Gte. Addition of ouabain, BaCl2 or diphenylamine-2-carboxylate to the internal bath inhibited Isc at unchanged Gte. Internal acetazolamide did not affect Isc or Gte across split gill lamellae. Unidirectional Na+ influx across isolated and perfused posterior gills, however, was reduced by internal acetazolamide by approximately 20% at constant Vte. The results suggest that posterior gills of hyperosmoregulating C. granulatus display a high conductance epithelium that actively absorbs NaCl in a coupled way by an electrogenic mechanism similar to that seen in the thick ascending limb of Henle's loop and, to a minor degree, by an electroneutral mechanism, presumably via apical Na+/H+and Cl-/HCO3--antiports.

Descripción: The porphyrias are a group of inherited metabolic disorders of heme biosynthesis which result from a partial deficiency in one of its seven specific enzymes, after its first and rate limiting enzyme, delta-aminolevulinic acid synthetase. They can be classified on the basis of their clinical manifestations into cutaneous, acute and mixed disorders. Acute intermittent porphyria (AIP) is the most common type of hepatic acute porphyrias, inherited as an autosomal dominant trait, caused by a defect in the gene which codifies for the heme enzyme porphobilinogen deaminase. Its prevalence in the Argentinean population is about 1:125,000. A partial deficiency in another enzyme, protoporphyrinogen oxidase, produces variegate porphyria (VP), the second acute porphyria most frequent in the Argentinean population (1:600,000). Here, we review all the mutations we have found in 46 AIP and 9 VP unrelated Argentinean patients. To screen for mutations in symptomatic patients, we have proposed a geneticresearch strategy.

Descripción: The main theorem of the previous paper is extended to the case where L = L(gij;Aiα;Ai α,j). References and notations are the same. © 1990 American Institute of Physics.

Descripción: Let G = (V, E) be a graph and d a positive integer. We study the following problem: for which labelings fE : E → Zd is there a labeling fV : V → Zd such that fE (i, j) = fV (i) + fV (j) (mod d), for every edge (i, j) ∈ E? We also explore the connections of the equivalent multiplicative version to toric ideals. We derive a polynomial algorithm to answer these questions and to obtain all possible solutions. © 2009 Elsevier B.V. All rights reserved.

Descripción: DNA size polymorphisms were utilized in a study of 24 natural populations of Ceratitis capitata Wiedemann (Diptera: Tephritidae) from Argentina. The first intron of alcohol dehydrogenase 1 gene (Adh1) was amplified using exon priming intron crossing-polymerase chain reaction. Three size variants were detected among the 307 samples analyzed. To better differentiate the size variants, further digestion of PCR products with the EcoRI restriction enzyme was carried out. Complete nucleotide sequences of the three-allele variants were obtained and single changes, insertions, deletions, and EcoRI recognition sites were located. Population allele frequencies were analyzed and a global mean heterozygosity (He) of 0.33 was obtained. In most populations, observed allelic frequencies conformed to Hardy-Weinberg expectations. Significant differences between provinces and sampling sites within these provinces, and among some populations were found. The average number of insects exchanged among populations (Nm) was estimated and high values were observed between Argentina and populations from two African countries (Morocco and Kenya), Australia, and Hawaii (Kauai). Pest introduction sources and dispersion patterns in Argentina are discussed based on these results as well as on available bibliographical data. © 2010 The Authors. Journal compilation © 2010 The Netherlands Entomological Society.

Descripción: In organello starch biosynthesis was studied using intact chloroplasts isolated from spinach leaves (Spinacia oleracea). Immunoblot analysis using a specific antiserum against the mitochondrial adenylate (ADP/ATP) translocator of Neurospora crassa shows the presence of an adenylate translocator protein in the chloroplast envelope membranes, similar to that existing in mitochondria and amyloplasts from cultured cells of sycamore (Acer pseudoplatanus). The double silicone oil layer-filtering centrifugation technique was employed to study the kinetic properties of adenylate transport in the purified chloroplasts; ATP, ADP, AMP, and most importantly ADP-Glc were shown to be recognized by the adenylate translocator. Similar to the situation with sycamore amyloplasts, only ATP and ADP-Glc uptake was inhibited by carboxyatractyloside, an inhibitor of the mitochondrial adenylate translocator. Evidence is presented to show that the ADP-Glc transported into the chloroplast stroma is utilized for starch synthesis catalyzed by starch synthase (ADP-Glc: 1,4-α-d-glucan 4-α-d-glucosyltransferase). The high activity of sucrose synthase producing ADP-Glc observed in the extrachloroplastic fractions suggests that starch biosynthesis in chloroplasts may be coupled with the direct import of ADP-Glc from the cytosol.

Descripción: A. santacatalinensis, found in exotic broadleaf woods in the environs of Buenos Aires, is described and illustrated. It belongs to subgenus Lanagaricus and is characterized by the chamois-like to velvety aspect of the pileus, the ochraceous yellowish color of the stipe with orange hues and detersile fibrils, and the centrifugally maturing process of the lamellae. A key to Argentinian species of the subgenus is proposed.

Descripción: The population structure, particularly growth, age, mortality and somatic production of the olivid snail Olivancillaria deshayesiana were investigated. Annual formation of internal shell growth marks was confirmed by the record of stable oxygen isotopes in the shell, which reflects seasonal patterns of water temperature. A von Bertalanffy growth model fitted to 81 size-atage data pairs, indicating that O. deshayesiana may attain 31 mm SL in about 10 years. The estimated total mortality Z and natural mortality M were 0.651 y-1 and 0.361 y -1, respectively. Fishing mortality F was 0.290 y -1, and the exploitation rate E was 0.445, indicating that this population was not overexploited at the time of the study. However, this situation may well change in the future, since the important prawn and shrimp fisheries (in intensity and scale) in the Mar del Plata area (38°20'S, 57°37'W) may indirectly affect the exploitation status of the studied population.

Descripción: Cerro Falkner is composed of granitic rocks underlying volcanic rocks of the Ventana Formation of Paleogene age. Those intrusives are graniodioritic in composition, metaluminous to peraluminous, subalkaline, with characteristics of volcanic arc granites that are concordant with other granitoids of the Cordillera Patagónica Septentrional's northern segment. The age obtained by the K-Ar method on tschemakitic amphiboles (125 ± 20 Ma) points out a minimun age from the lower Cretaceous for the Cerro Falkner's granodiorite. Therefore the use of the term Los Machis Formation (Upper Cretaceous) would be incorrect for these rocks. This age, allows us to relate the granitic rocks on the study area to the lower Cretaceous, coeval with the volcanism of the Divisadero Group.

Descripción: We examined the structure and characteristics of alarm calls uttered by nesting Southern House Wrens (Troglodytes musculus) during the 2007-2008 breeding seasons. We took multiple structural measures of 221 calls uttered by breeding individuals and used multivariate analyses to classify them. We recognized two distinctive alarm calls, named Type I and Type II. Type I calls were longer in duration and had higher frequencies than Type II calls; however, birds used Type II calls at a higher rate. Given the structural differences found between both call types; we propose that each call type has a different function, which remains to be tested. © The Neotropical Ornithological Society.

Descripción: Messenger RNA (mRNA) for enzymes involved in adrenal steroid biosynthesis are expressed in the brain, and the coded enzymes have been shown to be active. The expression of mRNA for the cytochrome P-450 enzyme aldosterone synthase, crucial for the final step in the synthesis of aldosterone and the synthesis of aldosterone was studied in several anatomic areas of the rat brain. Expression of the mRNA for the aldosterone synthase was demonstrated by RT-PCR/Southern blot in adrenal, aorta, hypothalamus, hippocampus, amygdala, cerebrum, and cerebellum. Incubation of brain minces from intact and adrenalectomized rats demonstrated the synthesis of corticosterone and aldosterone from endogenous precursors. Incubations of brain mince, with [1,23H]-deoxycorticosterone, followed by extraction and three different successive TLCs, demonstrated the presence of labeled aldosterone, corticosterone, and 18-hydroxy-deoxycorticosterone. Incubation, in the presence of 10 μM cortisol or metyrapone, inhibited the synthesis of aldosterone or both aldosterone and corticosterone, respectively. These studies indicate that the rat brain has the enzymatic machinery for the synthesis of adrenal corticosteroids and is capable of synthesizing aldosterone. Aldosterone synthesized in the brain might play a paracrine role in the regulation of blood pressure.

Descripción: Fil:Durán, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.

Descripción: The polysaccharides from cystocarpic Iridaea undulosa, soluble and insoluble in 2M potassium chloride, Cs and Ci, respectively, were treated with alkali and fractionated by precipitation with increasing concentrations of KCl. They were later separated by ion-exchange chromatography, to yield fractions enriched in an α-(1→6)-glucan, agaroids and carrageenans.

Descripción: The host-plant environment of phytophagous insects directly affects various aspects of an insect's life cycle. Interestingly, relatively few insect groups have specialized in the exploitation of plants in the Cactaceae family, potentially because of the chemical and ecological challenges imposed by these plants. The cactophilic Drosophila buzzatiiPatterson & Wheeler, 1942 is a well-studied model in evolutionary ecology, partially because of its ability to exploit toxic cactus hosts. Previous studies have shown a negative effect on performance when flies are reared in an alternative columnar cactus host of the genus Trichocereus, relative to its primary cactus host, Opuntia. These observations were attributed to the presence of alkaloids in Trichocereus tissues, a chemical deterrent to herbivores that indirectly affects Drosophila larvae; however, the putative toxic effect of alkaloids has never been tested directly in D. buzzatii. The present study is the first attempt to relate chemical extracts in Trichocereus terscheckii Britton & Rose, 1920 with detrimental effects on D. buzzatii. We assessed the effects of a crude alkaloid extract, rich in phenylethylamines, and a 'non-alkaloid fraction' on viability and adult wing morphology. Our results indicate that rearing larvae on an artificial diet containing different concentrations of the crude alkaloid extract decreased pupal viability and adult size in a concentration-dependent manner. We discuss the role of cactus alkaloids in the evolution of host-plant use in cactophilic flies. © 2013 The Linnean Society of London.

Descripción: Fil:Rozengurt, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.

Descripción: In this paper we classify a certain family of solutions of Lovelock gravity in the Chern-Simons (CS) case, in arbitrary (odd) dimension, d ≥ 5. The spacetime is characterized by admitting a metric that is a warped product of a two-dimensional spacetime M2 and an (a priori) arbitrary Euclidean manifold σd-2 of dimension d - 2. We show that the solutions are naturally classified in terms of the equations that restrict σd-2. According to the strength of such constraints we found the following branches in which σd-2 has to fulfill: a Lovelock equation with a single vacuum (Euclidean Lovelock Chern-Simons in dimension d - 2), a single scalar equation that is the trace of an Euclidean Lovelock CS equation in dimension d - 2, or finally a degenerate case in which σd-2 is not restricted at all. We show that all the cases have some degeneracy in the sense that the metric functions are not completely fixed by the field equations. This result extends the static five-dimensional case previously discussed in Dotti et al. [Phys. Rev. D76, 064038 (2007)]10.1103/PhysRevD.76.064038, and it shows that in the CS case, the inclusion of higher powers in the curvature does not introduce new branches of solutions in Lovelock gravity. Finally, we comment on how the inclusion of a non-vanishing torsion may modify this analysis. © 2013 American Institute of Physics.

Descripción: Transmission at the mouse neuromuscular junction normally relies on P/Q-type channels, but became jointly dependent on both N-and R-type Ca2+ channels when the P/Q-type channel α1A subunit was deleted. R-type channels lay close to Ca2+ sensors for exocytosis and IK(Ca) channel activation, like the P/Q-type channels they replaced. In contrast, N-type channels were less well localized, but abundant enough to influence secretion strongly, particularly when action potentials were prolonged. Our data suggested that active zone structures may select among multiple Ca2+ channels in the hierarchy P/Q>R>N. The α1A-/- neuromuscular junction displayed several other differences from wild-type: lowered quantal content but greater ability to withstand reductions in the Ca2+/Mg2+ ratio, and little or no paired-pulse facilitation, the latter findings possibly reflecting compensatory mechanisms at individual release sites. Changes in presynaptic function were also associated with a significant reduction in the size of postsynaptic acetylcholine receptor clusters.

Descripción: Pleiotropic effects of leptin have been identified in reproduction and pregnancy, particularly in the placenta, where it works as an autocrine hormone. In this work, we demonstrated that human chorionic gonadotropin (hCG) added to JEG-3 cell line or to placental explants induces endogenous leptin expression. We also found that hCG increased cAMP intracellular levels in BeWo cells in a dose-dependent manner, stimulated cAMP response element (CRE) activity and the cotransfection with an expression plasmid of a dominant negative mutant of CREB caused a significant inhibition of hCG stimulation of leptin promoter activity. These results demonstrate that hCG indeed activates cAMP/PKA pathway, and that this pathway is involved in leptin expression. Nevertheless, we found leptin induction by hCG is dependent on cAMP levels. Treatment with (Bu)2cAMP in combination with low and non stimulatory hCG concentrations led to an increase in leptin expression, whereas stimulatory concentrations showed the opposite effect. We found that specific PKA inhibition by H89 caused a significant increase of hCG leptin induction, suggesting that probably high cAMP levels might inhibit hCG effect. It was found that hCG enhancement of leptin mRNA expression involved the MAPK pathway. In this work, we demonstrated that hCG leptin induction through the MAPK signaling pathway is inhibited by PKA. We observed that ERK1/2 phosphorylation increased when hCG treatment was combined with H89. In view of these results, the involvement of the alternative cAMP/Epac signaling pathway was studied. We observed that a cAMP analogue that specifically activates Epac (CPT-OMe) stimulated leptin expression by hCG. In addition, the overexpression of Epac and Rap1 proteins increased leptin promoter activity and enhanced hCG. In conclusion, we provide evidence suggesting that hCG induction of leptin gene expression in placenta is mediated not only by activation of the MAPK signaling pathway but also by the alternative cAMP/Epac signaling pathway. © 2012 Maymó et al.

Descripción: To determine whether the stationary sigma factor, σS, influences polyhydroxyalkanoate metabolism in Pseudomonas putida KT2440, an rpoS-negative mutant was constructed to evaluate polyhydroxyalkanoate accumulation and expression of a translational fusion to the promoter region of the genes that code for polyhydroxyalkanoate synthase 1 (phaC1) and polyhydroxyalkanoate depolymerase (phaZ). By comparison with the wild-type, the rpoS mutant showed a higher polyhydroxyalkanoate degradation rate and increased expression of the translational fusion during the stationary growth phase. These results suggest that σS might control the genes involved in polyhydroxyalkanoate metabolism, possibly in an indirect manner. In addition, survival and oxidative stress assays performed under polyhydroxyalkanoate- and nonpolyhydroxyalkanoate- accumulating conditions demonstrated that the accumulated polyhydroxyalkanoate increased the survival and stress tolerance of the rpoS mutant. According to this, polyhydroxyalkanoate accumulation would help cells to overcome the adverse conditions encountered during the stationary phase in the strain that lacks RpoS. © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

Descripción: Aluminum (Al) and iron (Fe) share several physicochemical characteristics and they both bind to transferrin (Tf), entering the cell via Tf receptors (TfR). Previously, we found similar values of affinity constant for the binding of TfR to Tf carrying either Al or Fe. The competitive interaction between both metals prevented normal Fe incorporation into K562 cells and triggered the upregulation of Fe transport. In the present work we demonstrated that Al modified Fe uptake without affecting the expression of Tf receptors. Both TfR and TfR2 mRNA levels, evaluated by RT-PCR, and TfR antigenic sites, analyzed by flow cytometry, were found unchanged after Al exposure. In turn, Al did induce upregulation of non-Tf bound Fe (NTBI) uptake. This modulation was not due to intracellular Fe decrease since NTBI transport proved not to be regulated by Fe depletion. Unlike its behavior in the presence of Tf, Al was unable to compete with NTBI uptake, suggesting that both metals do not share the same alternative transport pathway. We propose that Al interference with TfR-mediated Fe incorporation might trigger the upregulation of NTBI uptake, an adaptation aimed at incorporating the essential metal required for cellular metabolism without allowing the simultaneous access of a potentially toxic metal. © 2004 Elsevier B.V. All rights reserved.

Descripción: Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by a gradual loss of motoneurons. The majority of ALS cases are associated with a sporadic form whose etiology is unknown. Several pieces of evidence favor autoimmunity as a potential contributor to sporadic ALS pathology. To gain understanding concerning possible antigens interacting with IgGs from sporadic ALS patients (ALS-IgGs), we studied immunoreactivity against neuromuscular junction (NMJ), spinal cord and cerebellum of mice with and without the Ca V2.1 pore-forming subunit of the P/Q-type voltage-gated calcium (Ca 2+) channel. ALS-IgGs showed a strong reactivity against NMJs of wild-type diaphragms. ALS-IgGs also increased muscle miniature end-plate potential frequency, suggesting a functional role for ALS-IgGs on synaptic signaling. In support, in mice lacking the Ca V2.1 subunit ALS-IgGs showed significantly reduced NMJ immunoreactivity and did not alter spontaneous acetylcholine release. This difference in reactivity was absent when comparing N-type Ca 2+ channel wild-type or null mice. These results are particularly relevant because motoneurons are known to be early pathogenic targets in ALS. Our findings add further evidence supporting autoimmunity as one of the possible mechanisms contributing to ALS pathology. They also suggest that serum autoantibodies in a subset of ALS patients would interact with NMJ proteins down-regulated when P/Q-type channels are absent. © 2011 International Society for Neurochemistry.

Descripción: Herein we present a detailed analysis of the optical response of artificial opal films realized employing a near-field scanning optical microscope in collection and transmission modes. Near-field patterns measured at the rear surface when a plane wave impinges on the front face are presented with the finding that optical intensity maps present a clear correlation with the periodic arrangement of the outer surface. Calculations based on the vector Korringa-Kohn-Rostoker method reproduce the different profiles experimentally observed as well as the response to the polarization of the incident field. These observations constitute the first experimental confirmation of the collective lattice resonances that give rise to the optical response of these three dimensional periodic structures in the high-energy range. © 2011 American Institute of Physics.

Descripción: The displacement of particles or probes in the cell cytoplasm as a function of time is characterized by different anomalous diffusion regimes. The transport of large cargoes, such as organelles, vesicles or large proteins, involves the action of ATP-consuming molecular motors. We investigate the motion of pigment organelles driven by myosin-V motors in Xenopus laevis melanocytes using a high spatio-temporal resolution tracking technique. By analyzing the turning angles (φ) of the obtained 2D trajectories as a function of the time lag, we determine the critical time of the transition between anticorrelated and directed motion as the time when the turning angles begin to concentrate around φ 0. We relate this transition with the crossover from subdiffusive to superdiffusive behavior observed in a previous work [5]. We also assayed the properties of the trajectories in cells with inhibited myosin activity, and we can compare the results in the presence and absence of active motors. © 2010 IOP Publishing Ltd.

Descripción: Puffs are localized Ca2+ signals that arise in oocytes in response to inositol 1,4,5-trisphosphate (IP3). They are analogous to the sparks of myocytes and are believed to be the result of the liberation of Ca2+ from the endoplasmic reticulum through the coordinated opening of IP3 receptor/channels clustered at a functional release site. In this article, we analyze sequences of puffs that occur at the same site to help elucidate the mechanisms underlying puff dynamics. In particular, we show a dependence of the interpuff time on the amplitude of the preceding puff, and of the amplitude of the following puff on the preceding interval. These relationships can be accounted for by an inhibitory role of the Ca2+ that is liberated during puffs. We construct a stochastic model for a cluster of IP3 receptor/channels that quantitatively replicates the observed behavior, and we determine that the characteristic time for a channel to escape from the inhibitory state is of the order of seconds. © 2006 by the Biophysical Society.

Descripción: Fil:Gómez, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.

Descripción: knowledge of the initial shape of strain markers allows calculation of the strain ellipsoid (axial relationships and orientation) when they show distorted geometries after deformation. This information is usually combined with the analysis of other micro- and mesostructures that constitute the dynamic framework in which the whole assemblage of structures developed. If only one deformational episode was responsible for all of them, the result is a highly coherent picture. In this paper, the quantification of strain and orientation of strain axes, is attempted on the basis of distorted hexagonal columns cropping out close to Puesto El Malabar (Pigüé, Sierras Australes de Buenos Aires). This columnar strucutre occurs in rhyolitic flows and tuffs of the Precambrian (?) basement in the area. It is apparent from this study that deformation in the central part of the north-western arc of Sierras Australes is markedly heterogeneous at all scales, with axial relationships giving values that record a variationin between almost no deformation and belts of high strain in which cleavage erases all evidence of columnar jointing. Micro- and mesostructural analysis shows dextral simple shear in plan view, oriented Az.145°-150° , and E-W stripes of sinistral shear that appear to be later and fewer than the earlier, one. Evidence of shear to the NE in vertical section has been found that is also heterogeneously developed. Secondary microstructures also show geometric relationships that are coherent with the general picture in the area. The regional significance of these data is analyzed in the regional picture, and it is concluded that it fits the previous model of an arcuate belt resulting from local development of conjugate megashears, with E-W (sinistral) and SW-NE (dextral) bands shaping the arc. © 1998 Asociación Geológica Argentina.

Descripción: We compare two statistical models of Alfvén solitons whose evolution is described by the one-dimensional derivative nonlinear Schrödinger (DNLS) equation, contrasting their predictions with solar wind observations. Both distribution functions give the same mean number of solitons. This confirms a previous calculation of Ponce Dawson and Ferro FontÁn of the number of solitons which evolve from an arbitrary initial condition. One of the distribution functions follows an exponential law with soliton energy and the other follows a power law; the latter gives better results than the former. Within these models, we can explain the variation of the observed spectra (spectral index, outer scale, and maximum value) with the heliocentric distance. This variation is related to the radial dependence of the mean level of modulational instability in the medium. Concerning the spectral index, our calculation improves that of Ovenden, Shah, and Schwartz, because an average over the soliton phases is included.

Descripción: We obtain a fast convergent series expansion for the Fermi-Dirac function Fσ(α) for - 10 ≤α≤ - 1. We give values of Fσ(α) for σ = n + 1/2 (n = 0,1,...,6) with α in the same range. © 1981 American Institute of Physics.

Descripción: In this work we present an anamorphic zoom system, based on liquid crystal displays, which allows changing the magnification and distortion of an image very quickly, without mechanical parts and keeping the output plane stationary. The anamorphic lenses that make up the optical processor are obtained by displaying a combination of convergent-divergent cylindrical lenses, with arbitrary orientations, onto the spatial light modulators. In order to illustrate the capabilities of the system, some experimental results are shown.

Descripción: The proopiomelanocortin gene (POMC) is expressed in the pituitary gland and the ventral hypothalamus of all jawed vertebrates, producing several bioactive peptides that function as peripheral hormones or central neuropeptides, respectively. We have recently determined that mouse and human POMC expression in the hypothalamus is conferred by the action of two 5′ distal and unrelated enhancers, nPE1 and nPE2. To investigate the evolutionary origin of the neuronal enhancer nPE2, we searched available vertebrate genome databases and determined that nPE2 is a highly conserved element in placentals, marsupials, and monotremes, whereas it is absent in nonmammalian vertebrates. Following an in silico paleogenomic strategy based on genome-wide searches for paralog sequences, we discovered that opossum and wallaby nPE2 sequences are highly similar to members of the superfamily of CORE-short interspersed nucleotide element (SINE) retroposons, in particular to MAR1 retroposons that are widely present in marsupial genomes. Thus, the neuronal enhancer nPE2 originated from the exaptation of a CORE-SINE retroposon in the lineage leading to mammals and remained under purifying selection in all mammalian orders for the last 170 million years. Expression studies performed in transgenic mice showed that two nonadjacent nPE2 subregions are essential to drive reporter gene expression into POMC hypothalamic neurons, providing the first functional example of an exapted enhancer derived from an ancient CORE-SINE retroposon. In addition, we found that this CORE-SINE family of retroposons is likely to still be active in American and Australian marsupial genomes and that several highly conserved exonic, intronic and intergenic sequences in the human genome originated from the exaptation of CORESINE retroposons. Together, our results provide clear evidence of the functional novelties that transposed elements contributed to their host genomes throughout evolution. © 2007 Santangelo et al.

Descripción: Modeling of broadband seismic waveforms recorded by global and Chilean networks for two moderate crustal earthquakes of the northwestern Sierras Pampeanas shows their focal mechanisms, depths and seismotectonic features. The magnitude Mw 5.8 earthquake on 28 May 2002, located in the eastern flank of the sierra de Velasco and the Mw 6.2 earthquake on 7 September 2004, with epicenter in the southwestern part of the sierra de Ambato, have shallow focal depths of 10 and 8 km, respectively. These results combined with the historical seismicity of the region allow us to estimate the deep structure of the Sierras Pampeanas in the study region. The seismic analyses together with interpretations of the surface structure and previous neotectonic studies ruled out extensional or strike slip deformation as the main responsible mechanism of the Present structure of this sector of the sierras de Ambato and Velasco in the northwestern Sierras Pampeanas. The comparison between the Nazca-South America plate convergence orientation as well as GPS velocities in the upper plate with the summation of the seismic moment tensor for the largest seismic energy released by crustal earthquakes of this region in the last 30 years, shows a clockwise rotation of 50° of the average P-axis orientation from the convergence orientation to the northeast suggesting important strain partition. This partition is controlled by the Eopaleozoic basement fabric, which has guided the orientation and vergence of the Andean faults.

Descripción: The first direct determination of the inertial range energy cascade rate, using an anisotropic form of Yaglom's law for magnetohydrodynamic turbulence, is obtained in the solar wind with multispacecraft measurements. The two-point mixed third-order structure functions of Elsässer fluctuations are integrated over a sphere in magnetic field-aligned coordinates, and the result is consistent with a linear scaling. Therefore, volume integrated heating and cascade rates are obtained that, unlike previous studies, make only limited assumptions about the underlying spectral geometry of solar wind turbulence. These results confirm the turbulent nature of magnetic and velocity field fluctuations in the low frequency limit, and could supply the energy necessary to account for the nonadiabatic heating of the solar wind. © 2011 American Physical Society.

Descripción: Rapidly rotating turbulent flow is characterized by the emergence of columnar structures that are representative of quasi-two-dimensional behavior of the flow. It is known that when energy is injected into the fluid at an intermediate scale L f, it cascades towards smaller as well as larger scales. In this paper we analyze the flow in the inverse cascade range at a small but fixed Rossby number, Ro f≈0.05. Several numerical simulations with helical and nonhelical forcing functions are considered in periodic boxes with unit aspect ratio. In order to resolve the inverse cascade range with reasonably large Reynolds number, the analysis is based on large eddy simulations which include the effect of helicity on eddy viscosity and eddy noise. Thus, we model the small scales and resolve explicitly the large scales. We show that the large-scale energy spectrum has at least two solutions: one that is consistent with Kolmogorov-Kraichnan-Batchelor-Leith phenomenology for the inverse cascade of energy in two-dimensional (2D) turbulence with a ∼k⊥-5/3 scaling, and the other that corresponds to a steeper ∼k⊥-3 spectrum in which the three-dimensional (3D) modes release a substantial fraction of their energy per unit time to the 2D modes. The spectrum that emerges depends on the anisotropy of the forcing function, the former solution prevailing for forcings in which more energy is injected into the 2D modes while the latter prevails for isotropic forcing. In the case of anisotropic forcing, whence the energy goes from the 2D to the 3D modes at low wave numbers, large-scale shear is created, resulting in a time scale τ sh, associated with shear, thereby producing a ∼k -1 spectrum for the total energy with the horizontal energy of the 2D modes still following a ∼k⊥-5/3 scaling. © 2012 American Physical Society.

Descripción: For over four decades, low frequency plasma and electromagnetic fluctuations have been observed in the solar wind (SW), making it the most completely studied case of magnetohydrodynamic turbulence in astrophysics, and the only one extensively and directly studied using in situ observations. Magnetohydrodynamic scale fluctuations in the SW are usually anisotropic with respect to the local mean magnetic field (B0). In this work, we present a study of turbulent properties in the inner heliosphere (solar wind between 0.3 and 1 AU) based on modeling in situ plasma and magnetic observations collected by Helios 1 and Helios 2 spacecraft throughout a solar minimum. We present preliminary results on the evolution of the spatial structure of the magnetic self-correlation function in the inner heliosphere. In particular we focus on the evolution of the integral length scale (λ) for magnetic fluctuations and on its anisotropy in the inertial range. As previously known from different studies, we confirm that near Earth λ ∥>λ⊥ (with λ∥ and λ⊥ representing the integral length in the parallel and perpendicular directions respect to B0, respectively). However, for lower distances to the Sun we found that λ∥<λ ⊥. Results presented here will help us to refine models used to describe the turbulence and waves activity in the inner heliosphere. © 2010 American Institute of Physics.

Descripción: Fil:Dasso, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.

Descripción: We show that the Pauli-Villars regularized action for a scalar field in a gravitational background in 1 + 1 dimensions has, for any value of the cutoff M, a symmetry which involves non-local transformations of the regulator field plus (local) Weyl transformations of the metric tensor. These transformations, an extension to the regularized action of the usual Weyl symmetry transformations of the classical action, lead to a new interpretation of the conformal anomaly in terms of the (non-anomalous) Jacobian for this symmetry. Moreover, the Jacobian is automatically regularized, and yields the correct result when the masses of the regulators tend to infinity. In this limit the transformations, which are non-local on a scale of 1/M, become the usual Weyl transformations of the metric. We also present the example of the chiral anomaly in 1 + 1 dimensions, showing that the Pauli-Villars regularized action has a non-local symmetry. This symmetry is similar to the one of (lattice) Ginsparg-Wilson fermions, with the ultraviolet cutoff playing the role of the inverse of the lattice spacing. (C) 2000 Elsevier Science B.V.

Descripción: In this work we studied the effect of NaCl on the thermodynamic and dynamic properties of supercooled water, for salt concentrations between 0.19 and 1.33mol kg-1, using molecular dynamic simulations for TIP5PE water model and ion parameters specially designed to be used in combination with this potential. We studied the isobaric heat capacity (Cp) temperature dependence and observed a maximum in Cp, occurring at Tm, that moves to lower temperature values with increasing salt concentration. Many characteristic changes were observed at scaled temperature TTm ∼ 0.96, namely a minimum in the density of the system, a reduction of the slope of the number of hydrogen bonds vs. temperature, and a crossover from Vogel-Tamman-Fulcher to Arrhenius dynamics. Finally, at low temperatures we observed that water dynamics become heterogeneous with an apparently common relationship between the fraction of immobile molecules and T/Tm for all studied systems. © 2011 American Institute of Physics.

Descripción: The organization of the cytoplasm is regulated by molecular motors, which transport organelles and other cargoes along cytoskeleton tracks. In this work, we use single particle tracking to study the in vivo regulation of the transport driven by myosin-V along actin filaments in Xenopus laevis melanophores. Melanophores have pigment organelles or melanosomes, which, in response to hormones, disperse in the cytoplasm or aggregate in the perinuclear region. We followed the motion of melanosomes in cells treated to depolymerize microtubules during aggregation and dispersion, focusing the analysis on the dynamics of these organelles in a time window not explored before to our knowledge. These data could not be explained by previous models that only consider active transport. We proposed a transport-diffusion model in which melanosomes may detach from actin tracks and reattach to nearby filaments to resume the active motion after a given time of diffusion. This model predicts that organelles spend -70% and 10% of the total time in active transport during dispersion and aggregation, respectively. Our results suggest that the transport along actin filaments and the switching from actin to microtubule networks are regulated by changes in the diffusion time between periods of active motion driven by myosin-V. © 2009 by the Biophysical Society.

Descripción: A highly sulfated 3-linked β-arabinan (Ab1) with arabinose in the pyranose form was obtained from green seaweed Codium vermilara (Bryopsidales). It comprised major amounts of units sulfated on C-2 and C-4 and constitutes the first polysaccharide of this type isolated in the pure form and fully characterized. Ab1 showed anticoagulant activity by global coagulation tests. Less sulfated arabinans obtained from the same seaweed have less or no activity. Ab1 exerts its activity through direct and indirect (antithrombin- and heparin cofactor II-mediated) inhibition of thrombin. Direct thrombin inhibition was studied in detail. By native PAGE, it was possible to detect formation of a complex between Ab1 and human thrombin (HT). Ab1 binding to HT was measured by fluorescence spectroscopy. CD spectra of the Ab1 complex suggested that ligand binding induced a small conformational change on HT. Ab1-thrombin interactions were studied by molecular dynamic simulations using the persulfated octasaccharide as model compound. Most carbohydrate-protein contacts would occur by interaction of sulfate groups with basic amino acid residues on the surface of the enzyme, more than 60% of them being performed by the exosite 2-composing residues. In these interactions, the sulfate groups on C-2 were shown to interact more intensely with the thrombin structure. In contrast, the disulfated oligosaccharide does not promote major conformational modifications at the catalytic site when complexed to exosite 1. These results show that this novel pyranosic sulfated arabinan Ab1 exerts its anticoagulant activity by a mechanism different from those found previously for other sulfated polysaccharides and glycosaminoglycans. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

Descripción: The trypanocidal action of green tea catechins against two different developmental stages of Trypanosoma cruzi is reported for the first time. This activity was assayed with the nonproliferative bloodstream trypomastigote and with the intracellular replicative amastigote parasite forms. An ethyl acetate fraction from Camellia sinensis green tea leaves, which contains most of the polyphenolic compounds and the maximal trypanocidal activity, was obtained by fractionation of the aqueous extract with organic solvents. The active compounds present in this extract were further purified by LH-20 column chromatography and were identified by high-performance liquid chromatography analysis with a photo diode array detector and gas chromatography coupled to mass spectroscopy. The following flavan-3-ols derivatives, known as catechins, were identified: catechin, epicatechin, gallocatechin, epigallocatechin, catechin gallate, epicatechin gallate, gallocatechin gallate, and epigallocatechin gallate. The purified compounds lysed more than 50% of the parasites present in the blood of infected BALB/c mice at concentrations as low as 0.12 to 85 pM. The most active compounds were gallocatechin gallate and epigallocatechin gallate, with minimal bactericidal concentrations that inhibited 50% of isolates tested of 0.12 and 0.53 pM, respectively. The number of amastigotes in infected Vero cells decreased by 50% in the presence of each of these compounds at 100 nM. The effects of the catechins on the recombinant T. cruzi arginine kinase, a key enzyme in the energy metabolism of the parasite, were assayed. The activity of this enzyme was inhibited by about 50% by nanomolar concentrations of catechin gallate or gallocatechin gallate, whereas the other members of the group were less effective. On the basis of these results, we suggest that these compounds could be used to sterilize blood and, eventually, as therapeutic agents for Chagas' disease.

Descripción: Bacteriophage Recombineering of Electroporated DNA (BRED) has been described for construction of gene deletion and point mutations in mycobacteriophages. Using BRED, we inserted a Phsp60-egfp cassette (1143 bp) into the mycobacteriophage D29 genome to construct a new reporter phage, which was used for detection of mycobacterial cells. The cassette was successfully inserted and recombinant mycobacteriophage purified. DNA sequencing of the cassette did not show any mutations even after several phage generations. Mycobacterium smegmatis mc2155 cells were infected with D29::Phsp60-egfp (MOI of 10) and evaluated for EGFP expression by microscopy. Fluorescence was observed at around 2 h after infection, but dissipated in later times because of cell lysis. We attempted to construct a lysis-defective mutant by deleting the lysA gene, although we were unable to purify the mutant to homogeneity even with complementation. These observations demonstrate the ability of BRED to insert c. 1 kbp-sized DNA segments into mycobacteriophage genomes as a strategy for constructing new diagnostic reporter phages. © 2013 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd.

Descripción: Imaging of samples by different microscopy techniques has produced a relevant impact in the development of new diagnosis techniques in biology, medicine and material science. In many biological, applications, where the sample changes or moves during the observation, a moving spot to track an identified sample is required. We introduce here an optical system that can perform, this tracking without mechanical components. The system, is based on the use of a high resolution liquid crystal on silicon (LCoS) device working as a mostly phase wave front modulator. The additional advantage of this system is performing the motion of the spot at video rate. In general, these devices produce coupled phase and amplitude modulation responses as a function of the applied voltage. This coupling effect deteriorates the response of those ideal optical elements designed as phase only or amplitude only functions. By means of an elliptical polarization light we can reduce the amplitude modulation and improve the phase modulation. We have experimentally found a configuration where the amplitude is almost constant while the phase reaches a high modulation. For this configuration we show how the spot can be moved through, focus plane by means of linear phases, or displaced out of this plane by using a quadratic phase. © 2008 American Institute of Physics.

Descripción: Single-cell cytoplasm sap (1-10 pL) was extracted by using a pressure probe glass microcapillary tip from tulip leaf and bulb and analyzed by UV-MALDI-TOF MS for free underivatized carbohydrate content. Three matrices including 2,5-dihydroxybenzoic acid (DHB), 2,4,6-trihydroxyacetophenone (THAP), and carbon nanotubes (CNTs) in positive ion mode were selected for analysis because of acceptable carbohydrate-related signal reproducibility. Disaccharide and oligosaccharide (up to 15 Hex when THAP was used, 11 Hex with DHB, and 7 Hex with CNTs) were detected in tulip bulb cell cytoplasm sample. When DHB was used as matrix, neutral carbohydrates were more abundantly detected as sodiated cations; the sugar-related signals, however, appeared as dominant potassiated cations when THAP and CNTs were used. Small amount of monosaccharide was also detected in bulb cell cytoplasm with CNTs as matrix. UV-MALDI-TOF MS of leaf cell extract resulted in high-resolution detection of hexose and disaccharide with DHB, THAP, and CNTs. © 2008 American Society for Mass Spectrometry.

Descripción: The title complex, aqua(2,2′-bipyridy-N,N′(3-thiapentanedioate-O,S,O′)zinc(II) tetrahydrate, [Zn(C4H4-O4S)(C10H8N 2)(H2O)].4H2O, is mononuclear with a coordination sphere consisting of one S, two N and three O atoms. The Zn atom is linked to a tridentate thiodiacetate ligand, a bidentate 2,2′-bipyridine ligand and an aqua ligand resulting in a slightly distorted octahedral coordination geometry. The molecular units are linked together in the crystal by an extensive hydrogen-bonding network and by intermolecular S⋯S interactions.

Descripción: Osmolarity not only plays a key role in celluar homeostasis but also challenges cell survival. The molecular understanding of osmosis has not yet been completely achieved, and the discovery of aquaporins as molecular entities involved in water transport has caused osmosis to again become a focus of research. The main questions that need to be answered are the mechanism underlying the osmotic permeability coefficients and the extent to which aquaporins change our understanding of osmosis. Here, attempts to answer these questions are discussed. Critical aspects of the state of the state of knowledge on osmosis, a topic that has been studied since 19th century, are reviewed and integrated with the available information provided by in vivo, in vitro and in silico approaches. © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Descripción: By means of some reasonable rules the operators that can represent arbitrary powers of the D'Alembertian and their corresponding Green's functions are defined. It is found which powers lead to the validity of Huygens' principle. The specially interesting case of powers that are half an odd integer in spaces of odd dimensionality, obey Huygens' principle, and can be expressed as iterated D'Alembertians of the retarded potential are discussed. Arbitrary powers of the Laplacian operator as well as their corresponding Green's functions are also discussed. © 1993 American Institute of Physics.

Descripción: We propose a new data structure for manipulating graphs, called h-graph, which is particularly suited for designing dynamic algorithms. The structure itself is simple, consisting basically of a triple of elements, for each vertex of the graph. The overall size of all triples is O(n+m), for a graph with n vertices and m edges. We describe algorithms for performing the basic operations related to dynamic applications, as insertions and deletions of vertices or edges, and adjacency queries. The data structure employs a technique first described by Chiba and Nishizeki [Chiba, Nishizeki, Arboricity and subgraph listing algorithms, SIAM J. Comput. 14 (1) (1985) 210223], and relies on the arboricity of graphs. Using the proposed data structure, we describe several dynamic algorithms for solving problems as listing the cliques of a given size, recognizing diamond-free graphs, and finding simple, simplicial and dominated vertices. These algorithms are the first of their kind to be proposed in the literature. In fact, the dynamic algorithms for the above problems lead directly to new static algorithms, and using the data structure we also design new static algorithms for the problems of counting subgraphs of size 4, recognizing cop-win graphs and recognizing strongly chordal graphs. The complexities of all of the proposed static algorithms improve over the complexities of the so far existing algorithms, for graphs of low arboricity. In addition, for the problems of counting subgraphs of size 4 and recognizing diamond-free graphs, the improvement is general. © 2011 Elsevier B.V. All rights reserved.

Descripción: The 15 species in the weevil genus Galapaganus Lanteri 1992 (Entiminae: Curculionidae: Coleoptera) are distributed on coastal Peril and Ecuador and include 10 flightless species endemic to the Galapagos islands. These beetles thus provide a promising system through which to investigate the patterns and processes of evolution on Darwin's archipelago. Sequences of the mtDNA locus encoding cytochrome oxidase subunit I (COI) were obtained from samples of seven species occurring in different ecological zones of the oldest south-eastern islands: San Cristobal, Espanola and Floreana, and the central island Santa Cruz. The single most parsimonious tree obtained shows two well-supported clades that correspond to the species groups previously defined by morphological characters. Based on a mtDNA clock calibrated for arthropods, the initial speciation separating the oldest species, G. galapagoensis (Linell) on the oldest island, San Cristobal, from the remaining species in the Galapagos occurred about 7.2 Ma. This estimate exceeds geological ages of the extant emerged islands, although it agrees well with molecular dating of endemic Galapagos iguanas, geckos and lizards. An apparent explanation for the disagreement between geological and molecular time-frames is that about 7 Ma there were emerged islands which subsequently disappeared under ocean waters. This hypothesis has gained support from the recent findings of 11 -Myr-old submarine seamounts (sunken islands), south-east of the present location of the archipelago. Some species within the darwini group may have differentiated on the extant islands, 1-5 Ma.

Descripción: The Arroyo La Chilca-Zanjón del Buitre alteration zone, San Rafael Massif, Mendoza. The Arroyo La Chilca-Zanjón del Buitre alteration zone is located in the San Rafael Massif (35°24'8" S; 68°19'25,8" W), Mendoza. Previous works defined preliminary this deposit as a porphyry copper system genetically linked to the gondwanan magmatism. This alteration zone is hosted by a sequence composed of ignimbritic breccias and ignimbrites of trachyandesitic composition with interbedded tuffaceous sandstones and andesitic lavas flows intruded by trachyandesitic-dacitic and andesitic hypabyssal bodies. The lithology and geochemistry of the volcanic sequence confirm the link with the Choiyoi Magmatic Cycle lower section (Lower Permian). The alteration zone consists of a potassic halo with a parageneses of K-feldspar-quartzmagnetite overprinted by phyllic alteration with an assemblage of quartz-muscovite-pyrite-(rutile) and quartz -pyrite veins. A late carbonatization process overprints both the potassic and phyllic alteration. Outside the alteration zone pyrite-chalcopyrite- galena-sphalerite veins with quartz gangue crop out. Metals geochemistry shows moderate Ag and Au anomalies and little Cu, Mo, Pb and Zn anomalies. Statistical analyses allow defining one Cu-Mo mineralizing stage and another Mo mineralizing stage only recognized in Zanjón del Buitre. Moreover, two Zn mineralizing stages, one of them probably genetically related to the Cu stage, were also recognized.

Descripción: The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that, besides mediating toxic responses, may have a central role in ovarian physiology. Studying the actions of AHR ligands on granulosa cells function, we have found that beta-naphthoflavone amplifies the comitogenic actions of FSH and 17beta-estradiol in a dose-dependent manner. This amplification was even greater in cells that overexpress the AHR and was reversed by cotreatment with the AHR antagonist alpha-naphthoflavone, suggesting that this effect is mediated by the AHR. The estrogen receptor is likewise implicated in this phenomenon, because a pure antiestrogen abolished the described synergism. However, the more traditional inhibitory AHR-estrogen receptor interaction was observed on the estrogen response element-driven transcriptional activity. On the other hand, alpha-naphthoflavone inhibited dose-dependently the mitogenic actions of FSH and 17beta-estradiol. Beta-naphthoflavone induced the expression of Cyp1a1 and Cyp1b1 transcripts, two well-characterized AHR-inducible genes that code for hydroxylases that metabolize estradiol to catecholestrogens. Nevertheless, the positive effect of beta-naphthoflavone on proliferation was not caused by increased metabolism of estradiol to catecholestrogens, because these compounds inhibited the hormonally stimulated DNA synthesis. This latter inhibition exerted by catecholestrogens suggests that these hydroxylases would play a regulatory point in granulosa cell proliferation. Our study indicates that AHR ligands modulate the proliferation of rat granulosa cells, and demonstrates for the first time that an agonist of this receptor is able to amplify the comitogenic action of classical hormones through a mechanism that might implicate a positive cross-talk between the AHR and the estrogen receptor pathways. © 2006 by the Society for the Study of Reproduction, Inc.

Descripción: Drill samples from the Carrizal uranium ore deposit located in Western Precordillera, Province of San Juan, Argentina, were studied by petrography, ore microscopy, X-ray diffraction, energy dispersive electron microscope and microprobe methods. A mesothermal ore paragenesis composed of arsenic-nickel-cobalt-uranium-bismuth minerals was identified. The established paragenetic sequence is pyrite-bismuthinite; nickeline-gersdorffite-rammelsbergite-pechblende, in an assemblage hosted by lithic breccia. A second mineralization stage produced a porphyry copper deposit, spatially but not genetically associated with the above described assemblage. © 1997 Asociación Geológica Argentina.

Descripción: Asr1, a tomato gene induced by abiotic stress, belongs to a family, composed by at least three members, involved in adaptation to dry climates. To understand the mechanism by which proteins of this family seem to protect cells from water loss in plants, we expressed Asr1 in the heterologous expression system Saccharomyces cerevisiae under the control of a galactose-inducible promoter. In a mutant yeast strain deficient in one component of the stress-responsive high-osmolarity glycerol (HOG) pathway, namely the MAP kinase Hog1, the synthesis of ASR1 protein restores growth under osmotic stress conditions such as 0.5 M NaCl and 1.2 M sorbitol. In contrast, the rescuing of this phenotype was less evident using a wild-type strain or the upstream MAP kinase kinase (Pbs2)-deficient strain. In both knock-out strains impaired in glycerol synthesis because of a dysfunctional HOG pathway, but not in wild-type, ASR1 led to the accumulation of endogenous glycerol in an osmotic stress-independent and unrestrained manner. These data suggest that ASR1 complements yeast HOG-deficient phenotypes by inducing downstream components of the HOG pathway. The results are discussed in terms of the function of ASR proteins in planta at the molecular and cellular level. Copyright © Physiologia Plantarum 2006.

Descripción: Asr (for ABA, stress, ripening) genes are exclusively found in the genomes of higher plants, and the encoded proteins have been found localized both to the nucleus and cytoplasm. However, before the mechanisms underlying the activity of ASR proteins can be determined, the role of these proteins in planta should be deciphered. Results from this study suggest that ASR is positioned within the signaling cascade of interactions among glucose, abscisic acid, and gibberellins. Tobacco (Nicotiana tabacum) transgenic lines with reduced levels of ASR protein showed impaired glucose metabolism and altered abscisic acid and gibberellin levels. These changes were associated with dwarfism, reduced carbon dioxide assimilation, and accelerated leaf senescence as a consequence of a fine regulation exerted by ASR to the glucose metabolism. This regulation resulted in an impact on glucose signaling mediated by Hexokinase1 and Snf1-related kinase, which would subsequently have been responsible for photosynthesis, leaf senescence, and hormone level alterations. It thus can be postulated that ASR is not only involved in the control of hexose uptake in heterotrophic organs, as we have previously reported, but also in the control of carbon fixation by the leaves mediated by a similar mechanism. © 2013 American Society of Plant Biologists. All Rights Reserved.

Descripción: Stack filters are a special case of non-linear filters. They have a good performance for filtering images with different types of noise while preserving edges and details. A stack filter decomposes an input image into several binary images according to a set of thresholds. Each binary image is then filtered by a Boolean function, which characterizes the filter. Adaptive stack filters can be designed to be optimal; they are computed from a pair of images consisting of an ideal noiseless image and its noisy version. In this work we study the performance of adaptive stack filters when they are applied to Synthetic Aperture Radar (SAR) images. This is done by evaluating the quality of the filtered images through the use of suitable image quality indexes and by measuring the classification accuracy of the resulting images. © 2011 Springer-Verlag.

Descripción: Fil:González, N.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.

Descripción: Background: Cognitive experiences during the early stages of life play an important role in shaping the future behavior in mammals but also in insects, in which precocious learning can directly modify behaviors later in life depending on both the timing and the rearing environment. However, whether olfactory associative learning acquired early in the adult stage of insects affect memorizing of new learning events has not been studied yet. Methodology: Groups of adult honeybee workers that experienced an odor paired with a sucrose solution 5 to 8 days or 9 to 12 days after emergence were previously exposed to (i) a rewarded experience through the offering of scented food, or (ii) a non-rewarded experience with a pure volatile compound in the rearing environment. Principal Findings: Early rewarded experiences (either at 1-4 or 5-8 days of adult age) enhanced retention performance in 9-12-day-conditioned bees when they were tested at 17 days of age. The highest retention levels at this age, which could not be improved with prior rewarded experiences, were found for memories established at 5-8 days of adult age. Associative memories acquired at 9-12 days of age showed a weak effect on retention for some pure pre-exposed volatile compounds; whereas the sole exposure of an odor at any younger age did not promote long-term effects on learning performance. Conclusions: The associative learning events that occurred a few days after adult emergence improved memorizing in middle-aged bees. In addition, both the timing and the nature of early sensory inputs interact to enhance retention of new learning events acquired later in life, an important matter in the social life of honeybees. © 2009 Arenas et al.

Descripción: In this work we present a system for autonomous discovery of asteroids, space trash and other moving objects. This system performs astronomical image data reduction based on an image processing pipeline. The processing steps of the pipeline include astrometric and photometric reduction, sequence alignment, moving object detection and astronomical analysis, making the system capable of discovering and monitoring previously unknown moving objects in the night sky. © Springer-Verlag 2013.

Descripción: We study in this paper the time evolution of the spreading process of a small drop in contact with a flat dry surface, using asymptotic techniques. We reduced the problem by solving a quasisteady self-similar macroscopic problem and matched with the precursor region solution, where the van der Waals forces are important. A final nonlinear third-order ordinary differential equation has been solved numerically using shooting methods based on the fourth-order Runge-Kutta techniques. We obtained how the capillary number changes when the drop size decreases with time. The evolution process then diverges slightly from that obtained using the spherical cap approximation. The influence of gravity is also considered for both hanging and sitting drops. © 1998 The American Physical Society.

Descripción: We study the asymptotic behavior for nonlocal diffusion models of the form ut = J * u - u in the whole RN or in a bounded smooth domain with Dirichlet or Neumann boundary conditions. In RN we obtain that the long time behavior of the solutions is determined by the behavior of the Fourier transform of J near the origin, which is linked to the behavior of J at infinity. If over(J, ̂) (ξ) = 1 - A | ξ |α + o (| ξ |α) (0 < α ≤ 2), the asymptotic behavior is the same as the one for solutions of the evolution given by the α / 2 fractional power of the Laplacian. In particular when the nonlocal diffusion is given by a compactly supported kernel the asymptotic behavior is the same as the one for the heat equation, which is yet a local model. Concerning the Dirichlet problem for the nonlocal model we prove that the asymptotic behavior is given by an exponential decay to zero at a rate given by the first eigenvalue of an associated eigenvalue problem with profile an eigenfunction of the first eigenvalue. Finally, we analyze the Neumann problem and find an exponential convergence to the mean value of the initial condition. © 2006 Elsevier SAS. All rights reserved.

Descripción: We numerically and theoretically investigate the evolution of the ridges and rifts produced by the convergent and divergent motions of two substrates over which an initially uniform layer of a Newtonian liquid rests. We put particular emphasis on the various asymptotic self-similar and quasi-self-similar regimes that occur in these processes. During the growth of a ridge, two self-similar stages occur; the first takes place in the initial linear phase, and the second is obtained for a large time. Initially, the width and the height of the ridge increase as t 1/2. For a very large time, the width grows as t 3/4, while the height increases as t 1/4. On the other hand, in the process of formation of a rift, there are three self-similar asymptotics. The initial linear phase is similar to that for ridges. The second stage corresponds to the separation of the current in two parts, leaving a dry region in between. Last, for a very large t, each of the two parts in which the current has separated approaches the self-similar viscous dam break solution. © 2008 American Institute of Physics.

Descripción: We study the asymptotic behaviour of best Sobolev constants on a compact manifold with boundary that we contract in k directions or to a point. We find in the limit best Sobolev constants for weighted Sobolev spaces of the limit manifold. © 2008 Elsevier Inc. All rights reserved.

Descripción: We investigate the relaxation of a strongly turbulent fluid to metastable states, in times much shorter than the dissipation time scale. Several simulations of decaying two-dimensional Navier-Stokes flows were performed, which show the relaxation to organized states dominated by coherent vortex structures of length scales comparable to the size of the system. In the case of periodic boundary conditions, the organized state is characterized by a strong correlation between vorticity and stream function, the second of which satisfies a sinh-Poisson equation quite accurately. However, in the case of free-slip boundary conditions the relaxed state does not display any significant correlation between its vorticity and its stream function. Notwithstanding, in both cases the role of nonlinearities is found to be essential even at these late stages of the evolution. However, we show that even severe truncations of a few (short wave number) nonlinearly coupled Fourier modes provide an accurate description of the long-term dynamics of the fluid. Therefore the dynamics of the flow in these metastable states is somewhere in between a strong turbulent regime and a (mostly linear) dissipative relaxation stage. © 1996 The American Physical Society.

Descripción: This paper discusses the general atmospheric circulation over Patagonia on the basis of the principal palaeoclimate forcings: continental drift, orography, variations in the greenhouse gases in the Earth's atmosphere, evolution of the atmosphere and the oceans, changes in the orbit of the Earth, albedo feedbacks, and the land surface. These processes affect climate on time scales of millions to hundreds of thousands of years. Additionally, orbital forcing has had a major influence on climate during the Quaternary. The palaeo-atmospheric circulation of Patagonia is analyzed for the Early to Late Jurassic, the Cretaceous, the Late Palaeocene-Eocene Thermal Maximum, the Tortonian-Oligocene cooling, the Pliocene, the Quaternary including the Last Glacial Maximum, the Holocene Optimum, and the last millennium changes. Alternative palaeo-atmospheric circulations from climatic modelling scenarios through the ages are reviewed and compared with proxy data. Detailed and updated reference information on the topics analyzed is also provided. © 2011 The Linnean Society of London.

Descripción: We study the existence of atomic decompositions for tensor products of Banach spaces and spaces of homogeneous polynomials. If a Banach space X admits an atomic decomposition of a certain kind, we show that the symmetrized tensor product of the elements of the atomic decomposition provides an atomic decomposition for the symmetric tensor product ⊗s, μ n X, for any symmetric tensor norm μ. In addition, the reciprocal statement is investigated and analogous consequences for the full tensor product are obtained. Finally we apply the previous results to establish the existence of monomial atomic decompositions for certain ideals of polynomials on X. © 2008 Elsevier Inc. All rights reserved.

Descripción: The Kustaanheimo-Stiefel transformation together with the well-known expansion of the kernel of an isotropic harmonic oscillator is used to generate the atomic orbitals of the nonrelativistic hydrogen atom in a four-dimensional Riemann space through the path integral formalism. Group theoretical implications of the present problem are briefly discussed. © 1983 American Institute of Physics.

Descripción: We applied a methodology capable of resolving the optical rotatory power into atomic contributions. The individual atomic contributions to the optical rotatory power and molecular chirality of the methylhydroperoxide are obtained via a canonical transformation of the Hamiltonian by which the electric dipolar moment operator is transformed to the acceleration gauge formalism and the magnetic dipolar moment operator to the torque formalism. The gross atomic isotropic contributions have been evaluated for the carbon, the nonequivalent oxygen, and the nonequivalent hydrogen atoms of methylhydroperoxide, employing a very large Gaussian basis set which is close to the Hartree-Fock limit. © 2008 American Institute of Physics.

Descripción: 2-Cys peroxiredoxins (2-Cys Prxs) are ubiquitous peroxidases with important roles in cellular antioxidant defense and hydrogen peroxide-mediated signaling. Post-translational modifications of conserved cysteines cause the transition from low to high molecular weight oligomers, triggering the functional change from peroxidase to molecular chaperone. However, it remains unclear how non-covalent interactions of 2-Cys Prx with metabolites modulate the quaternary structure. Here, we disclose that ATP and Mg2+ (ATP/Mg) promote the self-polymerization of chloroplast 2-Cys Prx (polypeptide 23.5 kDa) into soluble higher order assemblies (>2 MDa) that proceed to insoluble aggregates beyond 5mMATP. Remarkably, the withdrawal of ATP or Mg2+ brings soluble oligomers and insoluble aggregates back to the native conformation without compromising the associated functions. As confirmed by transmission electron microscopy, ATP/Mg drive the toroid-like decamers (diameter 13 nm) to the formation of large sphere-like particles (diameter ∼30 nm). Circular dichroism studies on ATP-labeled 2-Cys Prx reveal that ATP/Mg enhance the proportion of β-sheets with the concurrent decrease in the content of α-helices. In line with this observation, the formation of insoluble aggregates is strongly prevented by 2,2,2-trifluoroethanol, a cosolvent employed to induce α-helical conformations. We further find that the response of self-polymerization to ATP/Mg departs abruptly from that of the associated peroxidase and chaperone activities when two highly conserved residues, Arg129 and Arg152, are mutated. Collectively, our data uncover that non-covalent interactions of ATP/Mg with 2-Cys Prx modulate dynamically the quaternary structure, thereby coupling the non-redox chemistry of cell energy with redox transformations at cysteine residues. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

Descripción: Background: Research suggests that individuals with different attachment patterns process social information differently, especially in terms of facial emotion recognition. However, few studies have explored social information processes in adolescents. This study examined the behavioral and ERP correlates of emotional processing in adolescents with different attachment orientations (insecure attachment group and secure attachment group; IAG and SAG, respectively). This study also explored the association of these correlates to individual neuropsychological profiles. Methodology/Principal Findings: We used a modified version of the dual valence task (DVT), in which participants classify stimuli (faces and words) according to emotional valence (positive or negative). Results showed that the IAG performed significantly worse than SAG on tests of executive function (EF attention, processing speed, visuospatial abilities and cognitive flexibility). In the behavioral DVT, the IAG presented lower performance and accuracy. The IAG also exhibited slower RTs for stimuli with negative valence. Compared to the SAG, the IAG showed a negative bias for faces; a larger P1 and attenuated N170 component over the right hemisphere was observed. A negative bias was also observed in the IAG for word stimuli, which was demonstrated by comparing the N170 amplitude of the IAG with the valence of the SAG. Finally, the amplitude of the N170 elicited by the facial stimuli correlated with EF in both groups (and negative valence with EF in the IAG). Conclusions/Significance: Our results suggest that individuals with different attachment patterns process key emotional information and corresponding EF differently. This is evidenced by an early modulation of ERP components' amplitudes, which are correlated with behavioral and neuropsychological effects. In brief, attachments patterns appear to impact multiple domains, such as emotional processing and EFs. © 2013 Escobar et al.

Descripción: Pollination includes processes where water and/or solute movements must be finely regulated, suggesting participation of aquaporins. Using information available from different transcriptional profilings of Arabidopsis thaliana mature pollen, we showed that the only aquaporins that are selectively and highly expressed in mature pollen are two TIPs: AtTIP1;3 and AtTIP5;1. Pollen exhibited a lower number and more exclusive type of aquaporin expressed genes when compared to other single cell transcriptional profilings. When characterized using Xenopus oocyte swelling assays, AtTIP1;3 and AtTIP5;1 showed intermediate water permeabilities. Although they displayed neither glycerol nor boric acid permeability they both transported urea. In conclusion, these results suggest a function for AtTIP1;3 and AtTIP5;1 as specific water and urea channels in Arabidopsis pollen. © 2008 Federation of European Biochemical Societies.

Descripción: A regression approach was used to quantitatively estimate the attribution of the notable growth in the river flows of the Plata Basin during 1960-1999. The study was conducted in seven large basins that account for most of the Plata River discharge. Annual rainfall integrated over each basin and annual river flows at their closing points were used for the analysis. The contribution of rainfall changes during each of the three phases of El Niño-Southern Oscillation to total rainfall change in these basins was also calculated. The two main drivers for the generalized growth of the river flows were the increased precipitation and the decreased evaporation attributable to land use change, including deforestation of natural forest and crop switch from sugarcane and coffee trees to soybean. Other evaporation changes played a minor role. There was a north-south gradient in the respective importance of each driver, with land use change having greater weight in the northern basins and the precipitation increase in the southern ones. Thus, in the northern part of the Upper Paraná Basdespite the negative trend in precipitation there was a strong augment of the river flow caused by land use change. The contribution to the positive trend of the stream flows in the middle of the Plata Basin came from both land use change and increased precipitation. Finally, in the south, the Uruguay River flow change was basically due to the precipitation trend that was not only observed during the El Niño phase, but also during the Neutral phase. Only in the Middle Paraguay Basin was the shift to more frequent and intense El Niño events that took place in the 1970s an important factor in the contribution of precipitation to streamflow trends. © 2010 Royal Meteorological Society.

Descripción: Background: Eukaryotic DNA methylation is one of the most studied epigenetic processes, as it results in a direct and heritable covalent modification triggered by external stimuli. In contrast to mammals, plant DNA methylation, which is stimulated by external cues exemplified by various abiotic types of stress, is often found not only at CG sites but also at CNG (N denoting A, C or T) and CNN (asymmetric) sites. A genome-wide analysis of DNA methylation in Arabidopsis has shown that CNN methylation is preferentially concentrated in transposon genes and non-coding repetitive elements. We are particularly interested in investigating the epigenetics of plant species with larger and more complex genomes than Arabidopsis, particularly with regards to the associated alterations elicited by abiotic stress.Results: We describe the existence of CNN-methylated epialleles that span Asr1, a non-transposon, protein-coding gene from tomato plants that lacks an orthologous counterpart in Arabidopsis. In addition, to test the hypothesis of a link between epigenetics modifications and the adaptation of crop plants to abiotic stress, we exhaustively explored the cytosine methylation status in leaf Asr1 DNA, a model gene in our system, resulting from water-deficit stress conditions imposed on tomato plants. We found that drought conditions brought about removal of methyl marks at approximately 75 of the 110 asymmetric (CNN) sites analysed, concomitantly with a decrease of the repressive H3K27me3 epigenetic mark and a large induction of expression at the RNA level. When pinpointing those sites, we observed that demethylation occurred mostly in the intronic region.Conclusions: These results demonstrate a novel genomic distribution of CNN methylation, namely in the transcribed region of a protein-coding, non-repetitive gene, and the changes in those epigenetic marks that are caused by water stress. These findings may represent a general mechanism for the acquisition of new epialleles in somatic cells, which are pivotal for regulating gene expression in plants. © 2011 González et al; licensee BioMed Central Ltd.

Descripción: The pro-opiomelanocortin (POMC) gene is expressed in a subset of hypothalamic and hindbrain neurons and in pituitary melanotrophs and corticotrophs. POMC neurons release the potent opioid β-endorphin and several active melanocortins that control homeostasis and feeding behavior. POMC gene expression in the CNS is believed to be controlled by distinct cis- acting regulatory sequences. To analyze the transcriptional regulation of POMC in neuronal and endocrine cells, we produced transgenic mice carrying POMC27*, a transgene containing the entire 6 kb of the POMC transcriptional unit together with 13 kb of 5' flanking regions and 8 kb of 3' flanking regions: POMC27* was tagged with a heterologous 30 bp oligonucleotide in the third exon. In situ hybridization studies showed an accurate cell-specific pattern of expression of POMC27* in the arcuate nucleus and the pituitary. Hypothalamic mRNA-positive neurons colocalized entirely with β-endorphin immunoreactivity. No ectopic transgenic expression was detected in the brain. Deletional analyses demonstrated that neuron-specific expression of POMC transgenes required distal 5' sequences localized upstream of the pituitary- responsive proximal cis-acting elements that were identified previously. POMC27* exhibited a spatial and temporal pattern of expression throughout development that exactly paralleled endogenous POMC. RNase protection assays revealed that POMC27* expression mimicked that of POMC in different areas of the CNS and most peripheral organs with no detectable ectopic expression. Hormonal regulation of POMC27* and POMC was identical in the hypothalamus and pituitary. These results show that distal 5' sequences of the POMC gene located between -13 and -2 kb target expression into the CNS of transgenic mice in a precise neuron-specific, developmentally and hormonally regulated manner.

Descripción: The wheat spikelet meristem differentiates into up to 12 floret primordia, but many of them fail to reach the fertile floret stage at anthesis. We combined microarray, biochemical and anatomical studies to investigate floret development in wheat plants grown in the field under short or long days (short days extended with low-fluence light) after all the spikelets had already differentiated. Long days accelerated spike and floret development and greening, and the expression of genes involved in photosynthesis, photoprotection and carbohydrate metabolism. These changes started while the spike was in the light-depleted environment created by the surrounding leaf sheaths. Cell division ceased in the tissues of distal florets, which interrupted their normal developmental progression and initiated autophagy, thus decreasing the number of fertile florets at anthesis. A massive decrease in the expression of genes involved in cell proliferation, a decrease in soluble carbohydrate levels, and an increase in the expression of genes involved in programmed cell death accompanied anatomical signs of cell death, and these effects were stronger under long days. We propose a model in which developmentally generated sugar starvation triggers floret autophagy, and long days intensify these processes due to the increased carbohydrate consumption caused by the accelerated plant development. © 2008 The Authors.

Descripción: 1. 1. δ-Aminolaevulinate synthetase has been detected in extracts of soybean callus tissues and the enzyme activity reached its maximum when callus were 11 days old. 2. 2. The presence of a compound which seems to control δ-aminolaevulinate synthetase activity was demonstrated. The enzyme was present in the soluble fraction and was very labile. 3. 3. When crude extracts or 500 × g supernatant were stored at 4-6°, the apparent activity of δ-aminolaevulinate synthetase increased by as much as 3-6 times, while the activities of δ-aminolaevulinate dehydratase and succinyl-CoA synthetase did not significantly change during the storage. Activation was dependent on concentrations of cells suspensions during disruption and aging. 4. 4. Gel filtration with Sephadex G-25 of 2000 × g supernatants produced an enzyme fraction 30% more active. An increase in enzyme activity was observed when dark-grown callus were exposed to light. 5. 5. The addition of ATP, gibberellic acid and δ-aminolaevulinate to the culture media diminished activity; iron deficiency also produced an δ-aminolaevulinate synthetase less active. © 1971.