Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata

Fecha:

2010

Tipo de documento: 

info:eu-repo/semantics/article
info:ar-repo/semantics/artículo
info:eu-repo/semantics/publishedVersion

Formato:

application/pdf

Temas:

Diptera - EPIC-PCR - Exon priming intron crossing-polymerase chain reaction - Gene flow - Medfly - Migration patterns - Molecular markers - Population genetics - Tephritidae - allele - biomarker - fly - gene flow - genetic analysis - heterozygosity - migration - polymerase chain reaction - polymorphism - Ceratitis capitata - Diptera - Hexapoda - Tephritidae

Descripción:

DNA size polymorphisms were utilized in a study of 24 natural populations of Ceratitis capitata Wiedemann (Diptera: Tephritidae) from Argentina. The first intron of alcohol dehydrogenase 1 gene (Adh1) was amplified using exon priming intron crossing-polymerase chain reaction. Three size variants were detected among the 307 samples analyzed. To better differentiate the size variants, further digestion of PCR products with the EcoRI restriction enzyme was carried out. Complete nucleotide sequences of the three-allele variants were obtained and single changes, insertions, deletions, and EcoRI recognition sites were located. Population allele frequencies were analyzed and a global mean heterozygosity (He) of 0.33 was obtained. In most populations, observed allelic frequencies conformed to Hardy-Weinberg expectations. Significant differences between provinces and sampling sites within these provinces, and among some populations were found. The average number of insects exchanged among populations (Nm) was estimated and high values were observed between Argentina and populations from two African countries (Morocco and Kenya), Australia, and Hawaii (Kauai). Pest introduction sources and dispersion patterns in Argentina are discussed based on these results as well as on available bibliographical data. © 2010 The Authors. Journal compilation © 2010 The Netherlands Entomological Society.
Fil:Lanzavecchia, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Remis, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Cladera, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.

Fuente:

Entomol. Exp. Appl. 2010;136(1):53-65

Identificador:

http://hdl.handle.net/20.500.12110/paper_00138703_v136_n1_p53_Lanzavecchia
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00138703_v136_n1_p53_Lanzavecchia_oai