Analysis of persistence during intracellular actin-based transport mediated by molecular motors

En:

J. Phys. Conf. Ser. 2010;246

Fecha:

2010

Formato:

application/pdf

Tipo de documento:

info:eu-repo/semantics/conferenceObject
info:ar-repo/semantics/documento de conferencia
info:eu-repo/semantics/publishedVersion

Descriptores:

Cytology -  Anomalous diffusion -  Cell cytoplasm -  Function of time -  Molecular motors -  Spatio-temporal resolution -  Tracking techniques -  Turning angles -  Xenopus laevis

Descripción:

The displacement of particles or probes in the cell cytoplasm as a function of time is characterized by different anomalous diffusion regimes. The transport of large cargoes, such as organelles, vesicles or large proteins, involves the action of ATP-consuming molecular motors. We investigate the motion of pigment organelles driven by myosin-V motors in Xenopus laevis melanocytes using a high spatio-temporal resolution tracking technique. By analyzing the turning angles (φ) of the obtained 2D trajectories as a function of the time lag, we determine the critical time of the transition between anticorrelated and directed motion as the time when the turning angles begin to concentrate around φ 0. We relate this transition with the crossover from subdiffusive to superdiffusive behavior observed in a previous work [5]. We also assayed the properties of the trajectories in cells with inhibited myosin activity, and we can compare the results in the presence and absence of active motors. © 2010 IOP Publishing Ltd.
Fil:Despósito, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Bruno, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.

Identificador(es):

http://hdl.handle.net/20.500.12110/paper_17426588_v246_n_p_Pallavicini

Derechos:

info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar