De Xifra, E.A.W. - Del C. Batlle, A.M.

En:

Int. J. Biochem. 1974;5(2):129-135

Fecha:

1974

Formato:

application/pdf

Tipo de documento:

info:eu-repo/semantics/article
info:ar-repo/semantics/artículo
info:eu-repo/semantics/publishedVersion

Descriptores:

5 - 5′-dithio-bis-(2-nitrobenzoic acid) - enzyme inactivation - kinetics - molecular weight changes - soybean callus tissue - subunits - Succinyl-CoA synthetase - thiol groups

Descripción:

1. 1. The reaction between 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) and the SH groups of soybean callus succinyl-CoA synthetase has been investigated. At pH 8-6 and 25° C, four SH groups are titrable with DTNB in the native enzyme. No additional thiol groups have been revealed after unfolding of the protein with 8 M urea. 2. 2. The loss of enzymatic activity paralleled the decrease in the number of free SH groups. As with p-mercuribenzoate and other mercurials, reaction with DTNB also resulted in dissociation of the enzyme into subunits. © 1974.
Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.

Identificador(es):

http://hdl.handle.net/20.500.12110/paper_0020711X_v5_n2_p129_DeXifra

Derechos:

info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar

Descargar texto: paper_0020711X_v5_n2_p129_DeXifra.oai

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Cita bibliográfica:

De Xifra, E.A.W. (1974). Thiol groups of soybean callus succinyl CoA synthetase (info:eu-repo/semantics/article). [consultado: ] Disponible en el Repositorio Digital Institucional de la Universidad de Buenos Aires: <http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&cl=CL1&d=paper_0020711X_v5_n2_p129_DeXifra_oai>