cAMP analogs and selective inhibitors used to study low Km Mucor rouxii cAMP phosphodiesterase

En:

Int. J. Biochem. 1990;22(9):1047-1051

Fecha:

1990

Formato:

application/pdf

Tipo de documento:

info:eu-repo/semantics/article
info:ar-repo/semantics/artículo
info:eu-repo/semantics/publishedVersion

Descriptores:

1 ethyl 4 hydrazino 1h pyrazolo[3,4 b]pyridine 5 carboxylic acid ethyl ester -  4 (3 butoxy 4 methoxybenzyl) 2 imidazolidinone -  4,5 dihydro 6 [4 (1h imidazol 1 yl)phenyl] 5 methyl 3(2h) pyridazinone -  6 [4 (3 methylureido)phenyl] 3(2h) pyridazinone -  amrinone -  cilostamide -  cilostazol -  cyclic amp -  dipyridamole

Descripción:

1. 1. The sensitivity of partially purified low Km, phosphodiesterase (PDE) from Mucor rouxii to pharmacological agents and cAMP analogs was studied. The IC50 obtained were compared with those reported for PDEs from higher eukaryotes. 2. 2. The best inhibitors of the hydrolysis of 1 gmM cAMP were SQ 65.442 (IC50, c 10 μM), dipyridamol and CI 930. cGMP was not an inhibitor (IC50 > 1000 μm). 3. 3. The cAMP analogs were tested as inhibitors of the hydrolysis of 0. l μM cAMP. 8-Aminohexylamino cAMP was the best inhibitor with an IC50 of c 1 μM. 4. 4. A sedimentation profile of Mucor PDE was assayed in the presence of several pharmacological inhibitors and eAMP analogs. No isoforms with different sensitivity towards the inhibitors were detected. Forms with slightly different behaviour towards some cAMP analogs were observed. © 1990.
Fil:Tomes, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Moreno, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.

Identificador(es):

http://hdl.handle.net/20.500.12110/paper_0020711X_v22_n9_p1047_Tomes

Derechos:

info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar