Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose

En:

Biochim. Biophys. Acta Biomembr. 2005;1713(1):57-64

Fecha:

2005

Formato:

application/pdf

Tipo de documento:

info:eu-repo/semantics/article
info:ar-repo/semantics/artículo
info:eu-repo/semantics/publishedVersion

Descriptores:

Cholesterol -  DPPC -  DPPE -  Freeze-dried -  Phase transition -  cholesterol -  dipalmitoylphosphatidylcholine -  dipalmitoylphosphatidylethanolamine -  liposome

Descripción:

A study is presented of the role of cholesterol content on the gel-to-liquid crystalline phase transition of freeze-dried liposomes stabilized with trehalose, a well known lyoprotectant. The phospholipids considered in this work, DPPC and DPPE, belong to the two predominant phospholipid species found in numerous biological membranes. Cholesterol is found in abundance in mammalian plasma membranes. DSC measurements reveal that cholesterol-containing liposomes exhibit multiple phase transitions upon dehydration. Addition of trehalose to these systems lowers the phase transition temperature and limits the phase separation of the lipidic components upon freeze-drying. This work provides strong evidence for the effectiveness of trehalose in stabilizing cholesterol-containing membranes upon lyophilization. © 2005 Elsevier B.V. All rights reserved.
Fil:Schebor, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.

Identificador(es):

http://hdl.handle.net/20.500.12110/paper_00052736_v1713_n1_p57_Ohtake

Derechos:

info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar